Farklı Hücre Hatlarında KLK4 Gen İfadesinin Belirlenmesi

Yıl 2022, Cilt: 12 Sayı: 2, 207 - 215, 24.12.2022

Fatma Poyrazlı Sümeyye Aydogan Türkoğlu Derya Babacan Feray Köçkar

Öz

Kallikrein releated peptidaz (KLK) gen ailesi, insan genomundaki en büyük proteaz geni kümesi tarafından kodlanmaktadır ve yüksek oranda korunmuş 15 serin proteazdan oluşur. Bu ailenin doku kallikrein ve kallikrein ile ilişkili peptidazların en çok bilinen ve biyobelirteç olarak kullanılan üyesi prostata spesifik antijen (PSA- KLK3)’dir. Biyobelirteç olarak kullanılan diğer üyeleri ise, insan glandüler kallikrein 2 (KLK2) ve KLK4 (KLK-L1, PRSS17, AI2A1, ARM1, EMSP, EMSP1, PSTS TMPRSS2 ve prostaz) dür. PSA ve diğer KLK’lar arasındaki yapısal benzerlik göz önüne alındığında, ailenin kalan üyelerinin kanser biyobelirteçleri olarak potansiyel
rolü son yıllarda çokça araştırılmaktadır. Çalışmamızda PC-3/LNCaP (insan prostat karsinomu), HEP3B (insan hepatoselüler karsinomu), HT-29/SW480 (insan kolon karsinomu), ve HUVEC (insan umblikal ven endotel hücresi) hücre hatlarında KLK4’ün ifadesi mRNA ve protein düzeyinde çalışılmıştır. Çalışılan tüm hücre hatlarında ifadesinin olduğu, yine literatürle tutarlı olarak androjen bağımlı prostat kanserlerinde ise ifadesinin yüksek olduğu tespit edilmiştir. Ayrıca farklı evrelerde olan kolon kanseri hücre hatlarında,
kanser ilerleyişi ile ilişkili olarak KLK4 ifadesinin HT-29 hücrelerinde SW480 hücrelerine kıyasla yüksek bulunmuştur. Çalışmamız sonuçlarının KLK4 regülasyonunu hedefleyen kanser çalışmaları için hücre seçiminde yönlendirici olacağı düşünülmektedir

Anahtar Kelimeler

KLK4, Prostat kanseri, Kolon kanseri, Hepatoselüler karsinom, İnsan umblikal ven endotel hücresi, Human umbilical vein endothelial cell, Hepatocellular carcinoma, Colon cancer, Prostate cancer

Destekleyen Kurum

Tübitak

Proje Numarası

118Z369

Teşekkür

Bu çalışma TÜBİTAK 118Z369 nolu 1001 projesi ile desteklenmiştir. Bu çalışma 2018-2022 YÖK 100/2000 ve 2021-2022 BİDEB 2211-A Genel Yurt İçi Doktora Burs Programında bulunan Doktora öğrencisi Fatma POYRAZLI’ nın doktora tezinin bir bölümünü kapsamaktadır.

Expression of KLK4 Gene Expression Different Cell Lines

Öz

The highly conserved Kallikrein-related peptidase (KLK) gene family, encoded by the largest set of protease genes in the human genome, consists of 15 serine proteases. Prostate-specific antigen (PSA-KLK3) is the most well-known and biomarker member of tissue kallikrein and kallikrein-related peptidases. Others are human glandular kallikrein 2 (KLK2) and KLK4 (KLK-L1, PRSS17, AI2A1, ARM1, EMSP, EMSP1, PSTS TMPRSS2 and prostasis). Given the structural similarity between PSA and other KLKs, the potential role of remaining family members as cancer biomarkers has been extensively investigated in recent years. In our study,
the expression of KLK4 in PC-3/LNCaP (human prostate carcinoma), HEP3B (human hepatocellular carcinoma), HT-29/SW480 (human colon carcinoma), and HUVEC (human umbilical vein endothelial cell) cell lines was studied at the mRNA and protein level. KLK4 mRNA and protein expression were found in all studied cell lines and its expression is high in androgen-dependent prostate cancers (LNCaP), which is also consistent with the literature. In addition, a high level of KLK4 expression was found in HT-29 cells
than SW480 cells which are different stages of colon carcinoma cell lines, and this high-level expression is correlated with cancer progression. It is thought that the results of our study will be guided in the selection of cells for cancer studies targeting the regulation of KLK4.

Anahtar Kelimeler

Human umbilical vein endothelial cell, Hepatocellular carcinoma, Colon cancer, Prostate cancer, KLK4

Proje Numarası

118Z369

Kaynakça

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