Çam Balı Takviyeli Kitosan Esaslı Aktif Filmler

Yıl 2023, Cilt: 13 Sayı: 1, 62 - 71, 30.06.2023

Özge Kuyucak Pınar Terzioğlu Yusuf Sıcak

Öz

Yara örtüleri, özellikle kronik iyileşmeyen yaraların iyileşmesini hızlandırmada ve enfeksiyonu önlemede önemli rol oynamaktadır. Bal, biyouyumlu ve biyoaktiviteye sahip olduğu için modern klinik yara bakımı uygulamalarında değerlendirilmektedir. Son yıllarda, bal/ polimer hibrid malzemelerin yara iyileşme sürecini destekleyen terapötik özelliğe sahip ideal adaylar olabileceği düşünülmektedir. Bu çalışmada, çam balı (% 0-12.5) içeren kitosan esaslı filmler çözelti döküm yöntemi ile geliştirildi. Filmlerin morfolojisi ve yapısal özellikleri sırasıyla taramalı elektron mikroskobu ve Fourier Dönüşümlü Kızıl Ötesi Spektroskopisi (FTIR) ile belirlendi. Filmlerin antioksidan aktivitesi dört farklı metot ile belirlendi. FTIR spektrumları çam balının kitosan filmlere başarıyla dahil edildiğini gösterdi. Bal ilavesi filmlerin antioksidan aktivitesini arttırdı. Bu sonuçlar, çam balı içeren kitosan filmlerin yaraları tedavi etmek için umut verici adaylar olduğunu göstermektedir.

Anahtar Kelimeler

Bal, Kitosan film, Biyoaktivite

Destekleyen Kurum

TÜBİTAK 2209-B,

Proje Numarası

Başvuru numarası: 1139B411900084

Teşekkür

Bu çalışma TÜBİTAK 2209-B Üniversite Öğrencileri Araştırma Projeleri Destek Programı kapsamında desteklenmiştir (Başvuru numarası: 1139B411900084). Desteklerinden dolayı TÜBİTAK’a teşekkür ederiz.

Pine Honey Incorporated Chitosan Based Active Fims

Öz

Wound dressings play an important role in accelerating the healing of chronic non-healing wounds and preventing infection. Honey is evaluated in modern clinical wound care applications due to having biocompatible and bioactivity. In recent years, it is thought that honey / polymer hybrid materials can be ideal candidates with therapeutic properties that support the wound healing process. In this study, chitosan based films containing pine honey (0-12.5%) were developed by solution casting method. The morphology and structural properties of the films were determined by scanning electron microscopy and Fourier Transform Infrared Spectroscopy (FTIR), respectively. The antioxidant activity of the films was determined by four different methods. The FTIR spectra showed that pine honey was successfully incorporated into chitosan films. The addition of honey increased the antioxidant activity of the films. These results show that chitosan films containing pine honey are promising candidates for treating wounds.

Anahtar Kelimeler

Honey, Chitosan film, Bioactivity

Proje Numarası

Başvuru numarası: 1139B411900084

Kaynakça

• Abou-Okeil, A., Fahmy, H.M., El-Bisi, M.K., Ahmed-Farid,O.A. (2018). Hyaluronic acid/Na-alginate films as topical bioactive wound dressings. European Polymer Journal, 109,101-109. doi: 10.1016/j.eurpolymj.2018.09.003
• Afshari, M.J., Sheikh, N., Afarideh, H. (2015). PVA/CM-chitosan/honey hydrogels prepared by using the combined technique of irradiation followed by freeze-thawing. Radiation Physics and Chemistry, 113, 28-35. doi:10.1016/j.radphyschem.2015.04.023
• Apak, R., Güçlü, K., Özyürek, M., Karademir, S.E. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method, Journal of Agricultural and Food Chemistry, 52, 7970-7981. doi.org/10.1021/jf048741x
• Arslan, A., Şimşek, M., Dalkıranoğlu-Aldemir, S., Kazaroğlu, N. M., Gümüşderelioğlu, M. (2014). Honey-based PET or PET/chitosan fibrous wound dressings: effect of honey on electrospinning process. Journal of Biomaterials Science, Polymer Edition, 25 (10), 999-1012. doi:10.1080/09205063.2014.918455
• Bierhalz, A.C.K., Westin, C.B., Moraes, A.M. (2016). Comparison of the properties of membranes produced with alginate and chitosan from mushroom and from shrimp. International Journal of Biological Macromolecules, 91,496-504. doi:10.1016/j.ijbiomac.2016.05.095
• Blois, M.S. (1958). Antioxidant determinations by the use of a stable free radical, Nature, 181 (4617), 1199-1200. doi.org/10.1038/1811199a0
• Boucard,N.,Vitona,C., Agayb,D., Maric,E., Rogerc,T., Chancerelleb,Y., Domard, A. (2007).The use of physical hydrogels of chitosan for skin regeneration following third-degree burns. Biomaterials, 28 (24), 3478-3488. doi:10.1016/j.biomaterials.2007.04.021
• Bölgen, N., Demir, D., Yalçın, M. S., Özdemir, S. (2020). Development of Hypericum perforatum oil incorporated antimicrobial and antioxidant chitosan cryogel as a wound dressing material, International Journal of Biological Macromolecules, 161, 1581–1590. doi.org/10.1016/j.ijbiomac.2020.08.056
• Depan, D., Girase, B., Shah, J.S., Misra, R.D.K. (2011). Structure-process-property relationship of the polar graphene oxide-mediated cellular response and stimulated growth of osteoblasts on hybrid chitosan network structure nanocomposite scaffolds, Acta Biomatererialia, 7(9), 3432-3445. doi:10.1016/j.actbio.2011.05.019
• Dunford, C., Cooper, R., Molan, P. (2000).Using honey as a dressing forinfected skin lesions. Nursing Times,96,7–9.
• El-Kased, R. F., Amer, R. I., Attia, D., Elmazar, M. M. (2017). Honey-based hydrogel: In vitro and comparative In vivo evaluation for burn wound healing. Scıentıfıc Reports, 7: 9692, doi:10.1038/s41598-017-08771-8.
• Escárcega-Galaz, A.A., Sánchez-Machado, D. I., López-Cervantes, J., Sanches-Silva, A., Madera-Santana, T. J., Paseiro-Losada, P. (2018). Mechanical, structural and physical aspects of chitosan-based films as antimicrobial dressings. International Journal of Biological Macromolecules, 116, 472-481. doi:10.1016/j.ijbiomac.2018.04.149
• Fan, M., Hu, Q., Shen, K. (2009). Preparation and structure of chitosan soluble in wide pH range. Carbohydrate Polymers, 78 (1),66-71. doi:10.1016/j.carbpol.2009.03.031
• Gok, S., Severcan, M., Goormaghtigh, E., Kandemir, I., Severcan, F. (2015). Differentiation of Anatolian honey samples from different botanical origins by ATR-FTIR spectroscopy using multivariate analysis. Food Chemistry, 170, 234-240. doi:10.1016/j.foodchem.2014.08.040
• Gómez Chabala, L. F., Cuartas, C.E.E., López, M. E.L. (2017). Release Behavior and Antibacterial Activity of Chitosan/Alginate Blends with Aloe vera and Silver Nanoparticles. Marine Drugs, 15, 328. doi:10.3390/md15100328
• Güneş, Y.Ü., Eşer, İ. (2006). Nemli yara iyileşmesi ve oklusif pansumanların yara iyileşmesindeki önemi. Cumhuriyet Üniversitesi Hemşirelik Yüksekokulu Dergisi, 10 (2), 57-65.
• Kamel, N. A., Abd El-messieh, S. L., Saleh, N. M. (2017). Chitosan/banana peel powder nanocomposites for wound dressing application: Preparation and characterization. Materials Science and Engineering: C, 72, 543-550. doi:10.1016/j.msec.2016.11.104
• Khaleghi, M., Mani, F., Salimi, H., Hajibeygi, M., Pashazadeh, R., Zayerzadeh, E., Babanejad, N., Shabanian, M. (2018). Synthesis and characterization of new honey incorporated double‐network hydrogels based on poly(vinyl alcohol) and acylated chitosan. Advences in Polymer Technology, 37(8),3596-3606. doi:10.1002/adv.22144
• Kheradvar, S. A., Nourmohammadi, J., Tabesh, H., Bagheri, B. (2018). Starch nanoparticle as a vitamin E-TPGS carrier loaded in silk fibroin-poly(vinylalcohol)-Aloevera nanofibrous dressing. Colloids and Surfaces B: Biointerfaces, 166, 9-16. doi: 10.1016/j.colsurfb.2018.03.004
• López-Iglesias, C.,Barros, J., Ardao, I., Monteiro, F. J., Alvarez-Lorenzo, C., Gómez-Amoza, J. L., García-González, C.A. (2019).Vancomycin-loaded chitosan aerogel particles for chronic wound applications. Carbohydrate Polymers, 204, 223-231. doi:10.1016/j.carbpol.2018.10.012
• Marco, G. J. (1968). A rapid method for evaluation of antioxidants. Journal of the American Oil Chemists’ Society, 45(9), 594–598. doi.org/10.1007/BF02668958
• Mazloom-Jalali, A., Shariatinia, Z., Tamai, I.A., Pakzad, S.R., Malakootikhah, J. (2020). Fabrication of chitosan–polyethylene glycol nanocomposite films containing ZIF-8 nanoparticles for application as wound dressing materials. International Journal of Biological Macromolecules, 153,421-432. doi:10.1016/j.ijbiomac.2020.03.033
• Nasuti, C., Gabbianelli, R., Falcioni, G., Cantalamessa, F. (2006). Antioxidative and gastroprotective activities of anti-inflammatory formulations derived from chestnut honey in rats, Nutrition Research, 26, 130-137. doi.org/10.1016/j.nutres.2006.02.007
• Nguyen, H.T.L., Katopo, L., Pang, E., Mantri, N., Kasapis, S. (2019). Structural Variation in Gelatin Networks From Low to High-Solid Systems Effected by Honey Addition. Food Research International, 121, 319-325. doi:10.1016/j.foodres.2019.03.048
• Öztürk, M., Aydoğmuş-Öztürk, F., Duru, M. E., Topçu, G. (2007). Antioxidant activity of stem and root extracts of Rhubarb (Rheum ribes): An edible medicinal plant. Food Chemistry, 103(2), 623–630. doi.org/10.1016/j.foodchem.2006.09.005
• Pataca, L.C.M., Neto, W.B., Marcucci, M.C., Poppi, R.J. (2007). Determination of Apparent Reducing Sugars, Moisture and Acidity in Honey by Attenuated Total Reflectance-Fourier Transform Infrared Spectrometry. Talanta, 71(5), 1926-1931. doi: 10.1016/j.talanta.2006.08.028.
• Pita-Calvo, C., Guerra-Rodriguez, M.E., Vazquez M., (2017). Analytical methods used in the quality control of honey, Journal of Agricultural and Food Chemistry, 65, 690-703. doi.org/10.1021/acs.jafc.6b04776
• Priyadarshi, R., Kumar, S.B., Negi, Y. S., (2018). Chitosan film incorporated with citric acid and glycerol as an active packaging material for extension of green chilli shelf life. Carbohydrate Polymers, 195,329-338. doi: 10.1016/j.carbpol.2018.04.089
• Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. Free Radical Biology & Medicine, 26, 1231-1237. doi.org/10.1016/S0891-5849(98)00315-3
• Ribeiro, M.P., Espiga, A., Silva, D., Baptista, P., Henriques, J., Ferreira, C., Silva, J.C., Borges, J.P., Pires, E., Chaves, P., & Correia, I.J. (2009). Development of a new chitosan hydrogel for wound dressing. Wound Repair and Regeneration, 17(6), 817-824. doi: 10.1111/j.1524-475X.2009.00538.x.
• Sasikala, L., Rathinamoorthy R., Dhurai B. (2018). Optimization of process conditions for chitosan-manuka honey film as wound contact layer for wound dressings. Wound Medicine, 23,11-21. doi:10.1016/j.wndm.2018.09.007
• Shepherd, R., Reader, S., Falshaw, A. (1997). Chitosan functional properties. Glycoconjugate Journal, 14, 535-542. doi:10.1023/A:1018524207224.
• Siripatrawan, U., Vitchayakitti, W. (2016). Improving functional properties of chitosan films as active food packaging by incorporating with propolis. Food Hydrocolloids, 61, 695-702. doi:10.1016/j.foodhyd.2016.06.001
• Stashak, T.S.,Farstvedt, E., Othic, A. (2004). Update on wound dressings: Indications and bestuse. Clinical Techniques in Equine Practice, 3(2),148-163. doi:10.1053/j.ctep.2004.08.006
• Vela, L., de Lorenzo, C., Pérez, R.A. (2007). Antioxidant capacity of Spanish honeys and its correlation with polyphenol content and other physicochemical properties, Journal of the Science of Food and Agriculture, 87, 1069-1075. doi.org/10.1002/jsfa.2813
• Wang, T., Zhu,X.K., Xue,X.T., Wu, D.Y. (2012). Hydrogel sheets of chitosan, honey and gelatin as burn wound dressings. Carbohydrate Polymers, 88,75-83. doi:10.1016/j.carbpol.2011.11.069
• Yusof, N., Hafiza, A.H.A., Zohdi, R.M., Bakar,M.Z.A., (2007). Development of honey hydrogel dressing for enhanced wound healing. Radiation Physics and Chemistry, 76 (11),1767-1770. doi:10.1016/j.radphyschem.2007.02.107