Accurate, Simple and Rapid Determination of Climbazole as Fungicide in Tap Water Samples by Gas Chromatography-Mass Spectrometry

Yıl 2025, Cilt: 15 Sayı: 2, 68 - 75, 21.07.2025

Tuğçe Göver

Öz

This study describes the identification and quantification of climbazole, a fungicide, by using gas chromatography-mass spectrometry (GC-MS). A proper temperature program was applied for the efficient separation in the gas chromatography system for precise and accurate determination of climbazole. The analysis time for climbazole detection was 7.20 minutes. The identifier and quantifier ions were determined the ion peak with the highest relative abundance for climbazole in the National Institute of Standards and Technology (NIST) library, and the sharp peak at minute 4.95 in the chromatogram obtained in this ion peak was confirmed to belong to climbazole in the library. The analytical system provides linear working range between 2.05 and 99.37 mg/kg. Under the constant instrumental conditions, the limit of detection and limit of quantitation of climbazole were recorded as 0.60 and 2.01 mg/kg, respectively. Accuracy of the method was validated by performing recovery experiments on tap water samples. The climbazole was not detected in the two different tap water samples used in the recovery experiments. On the other hand, percent recovery results were calculated for spiked tap water samples by using an external calibration technique. The recovery values obtained for different climbazole concentrations were in the range of 87.7% - 114.4%. These results indicate the applicability and accuracy of the developed method in the determination of climbazole at water samples.

Anahtar Kelimeler

Climbazole , fungicide , gas chromatography , tap water

Teşekkür

The author is grateful to Bakırdere Research Group/Yıldız Technical University for providing the experimental opportunity to carry out this study.

Gaz Kromatografisi-Kütle Spektrometresi ile Musluk Suyu Örneklerinde Fungisit Olarak Klimbazolün Doğru, Basit ve Hızlı Tayini

Öz

Bu çalışmada, bir fungisit olan klimbazolün gaz kromatografisi-kütle spektrometrisi (GC-MS) kullanılarak belirlenmesi ve miktar tayini anlatılmaktadır. Klimbazolün hassas ve doğru tayini için gaz kromatografi sisteminde etkin bir ayırmanın yapılabilmesi amacıyla uygun sıcaklık programı uygulandı. Klimbazolün tayini için hızlı analiz süresi (7.20 dk) gerçekleştirildi. Tanımlayıcı ve niceleyici iyonlar, Ulusal Standartlar ve Teknoloji Enstitüsü (NIST) kütüphanesinde klimbazol için en yüksek bağıl bolluğa sahip iyon piki belirlendi ve bu iyon pikinde elde edilen kromatogramda 4.95 dakikadaki keskin pikin kütüphanede klimbazole ait olduğu doğrulandı. Analitik sistem 2.05 ile 99.37 mg/kg arasında oldukça iyi doğrusal çalışma aralığı sağlamaktadır. Sabit enstrümantal koşullar altında, klimbazolün gözlenebilme sınırı (LOD) ve tayin sınırı (LOQ) sırasıyla 0.60 ve 2.01 mg/kg olarak belirlendi. Yöntemin doğruluğu musluk suyu örneklerinde geri kazanım deneyleri yapılarak doğrulandı. Geri kazanım deneylerinde kullanılan iki farklı musluk suyu örneğinde klimbazol belirlenmedi. Öte yandan, standart ilaveli musluk suyu numuneleri için harici kalibrasyon tekniği kullanılarak yüzde geri kazanım sonuçları hesaplandı. Klimbazolün farklı konsantrasyonları için geri kazanım sonuçlarının %87.7 ile %114.4 arasında değiştiği görüldü. Bu sonuçlar, klimbazol tayini için geliştirilen yöntemin kompleks su örneklerinde bile uygulanabilirliğini ve doğruluğunu göstermektedir.

Anahtar Kelimeler

Klimbazol , fungisit , gaz kromatografisi , musluk suyu

Kaynakça

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