Voltammetric Determination of Guaifenesin on Poly (Nile blue) Modified Glassy Carbon Electrode in Pharmaceutical Dosage Forms

Abstract

The electro-oxidation behavior of expectorant drug guaifenesin (GUF) was studied on poly(Nile blue) modified glassy carbon electrode by cyclic voltammetry and differential pulse voltammetry. Camsı karbon elektrot was modified with electropolymerization of Nile blue for sensitive determination of GUF with voltammetric methods. The current peaks for GUF occurred at around 1.130 V for differential pulse voltammetry when the potential was scanned in the positive direction. The oxidation process of GUF has shown irreversible and diffusion controlled behavior. The linear response has been obtained in the range from 6 × 10^-7 to 1 × 10^-4 M with the limit of detection 1.24 × 10^-8 for differential pulse voltammetry in 0.1 M phosphate buffer solution at pH 3.0. Fully validated differential pulse voltammetry was successfully applied for the determination of GUF from pharmaceutical dosage form and obtained satisfying results.

Keywords

• Guaifenesin,poly(Nile blue) modified glassy carbon electrode,voltammetry.

Guaifenesinin Poli(Nil Mavisi) ile Modifiye Edilmiş Camsı Karbon Elektrot ile Voltametrik Analizi ve Farmasötik Dozaj Formundan Tayini

Öz

Ekspektorant bir ilaç etken maddesi guaifenesin (GUF) elektro-oksidasyon davranışı poli(Nil mavisi) modifiye camsı karbon elektrotta dönüşümlü voltametri (DV) ve diferansiyel puls voltametri (DPV) ile çalışıldı. Camsı karbon elektrot, GUF’un voltametrik metodlarla hassas tayini için, Nil mavisinin elektropolimerizasyonu ile modifiye edildi. Potansiyel pozitif yönde tarandığında GUF’un pik akımı 1.130 V civarinda DPV ile oluştu. GUF’un oksidasyon prosesi tersinmez ve difüzyon kontrollü davranış gösterdi. DPV için doğrusal cevaplar                             6 × 10^-7- 1 × 10^-4 M, 1.24 × 10^-8 yakalama alt sınırı (YAS) ile 0.1 M fosfat tamponu (FT) pH 3.0 içinde elde edildi. Tamamen valide edilmiş DPV başarılı bir şekilde GUF’un farmasötik dozaj formundan miktar tayini için uygulandı ve memnun edici sonuçlar elde edildi.

Anahtar Kelimeler

• Guafenesin,poli(Nil mavisi) modifiye camsı karbon elektrot,voltametri

Kaynakça

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