The Acute Effect of Fructose on Cardiac Hemodynamic Responses and Infarcted Area in Isolated Rat Heart During Ischemia-Reperfusion

Yıl 2023, Cilt: 26 Sayı: 1, 7 - 13, 21.03.2023

Orkide Palabıyık Muhammed Ali Aydın , Ecem Büşra Değer Selçuk Korkmaz Selma Arzu Vardar

Öz

Introduction: This study aimed to investigate the effects of fructose on cardiac hemodynamics and infarct size and the role of the antioxidant mechanism in these effects in isolated rat hearts undergoing ischemiareperfusion.

Patients and Methods: Isolated hearts obtained from female Wistar rats were perfused with Krebs-Henseleit solution containing 12 mM glucose or solution containing 12 mM fructose or 48 mM fructose and underwent lowflow ischemia followed by reperfusion on the Langendorff apparatus. Left ventricular developed pressure (LVDP), timedependent pressure changes (dp/dt max, dp/dt min) and heart rates were recorded at the 1st, 15th and 120th minutes of reperfusion following ischemia, and the percentage of the infarct area and the size of the risk area were determined. At the end of the reperfusion, total oxidant capacity (TOS), malondialdehyde (MDA) and glutathione (GSH) levels were examined in perfusion fluid samples.

Results: Basal dp/dt max values were lower in the high fructose group compared to the glucose group (p= 0.032). When the hearts were perfused with 12 mM fructose, a significant increase was observed in the percentage of the ischemic area and risk area compared to equivalent glucose and high fructose (p< 0.001 and p< 0.001, respectively). MDA, GSH and TOS values were comparable in all groups.

Conclusion: The present study shows that fructose perfusion plays a role in reduced ventricular contractile function relative to glucose in isolated rat hearts. This reduction triggered by fructose appears to be independent of antioxidant mechanisms. Furthermore, fructose perfusion at glucose-equivalent concentration causes a greater increase in infarct area in ischemic hearts, whereas an increase in fructose concentration appears to prevent this effect.

Anahtar Kelimeler

Fructose, ischemia, reperfusion, heart

Kaynakça

• 1. Miller A, Adeli K. Dietary fructose and the metabolic syndrome. Curr Opin Gastroenterol 2008;24(2):204-9.
• 2. Bray G. Fructose: Should we worry? Int J Obesity 2008;32(7):127-31.
• 3. Lloyd-Jones D, Adams RJ, Brown TM, Carnethon M, Dai S, De Simone G, et al. Executive summary: Heart disease and stroke statistics-2010 update: Report from the American Heart Association. Circulation 2010;121(7):948-54.
• 4. Shah SR, Hochberg CP, Pinto DS, Gibson CM. Reperfusion strategies for ST-elevation myocardial infarction. Curr Cardiol Rep 2007;9(4):281-8.
• 5. Haghi J, Eteraf-Oskouei T, Najafi M. Effects of postconditioning with fructose on arrhythmias and the size of infarct caused by global ischemia and reperfusion in isolated rat heart. Adv Pharm Bull 2018;8(1):57.
• 6. Wang J, Li J. Activated protein C: A potential cardioprotective factor against ischemic injury during ischemia/reperfusion. Am J Transl Res 2009;1(4):381.
• 7. Mellor KM, Bell JR, Wendt IR, Davidoff AJ, Ritchie RH, Delbridge LM. Fructose modulates cardiomyocyte excitation-contraction coupling and Ca2+ handling in vitro. PLoS One 2011;6(9):e25204.
• 8. Moens A, Claeys M, Timmermans J, Vrints C. Myocardial ischemia/reperfusion-injury, a clinical view on a complex pathophysiological process. Int J Cardiol 2005;100(2):179-90.
• 9. Tappy L, Lê KA. Metabolic effects of fructose and the worldwide increase in obesity. Physiol Rev 2010;90(1):23-46.
• 10. Cha SH, Wolfgang M, Tokutake Y, Chohnan S, Lane MD. Differential effects of central fructose and glucose on hypothalamic malonyl-CoA and food intake. Proc Natl Acad Sci USA 2008;105(44):16871-5.
• 11. Morel S, Berthonneche C, Tanguy S, Toufektsian MC, Foulon T, De Lorgeril M, et al. Insulin resistance modifies plasma fatty acid distribution and decreases cardiac tolerance to in vivo ischaemia/reperfusion in rats. Clin Exp Pharmacol Physiol. 2003;30(7):446-51.
• 12. Jordan JE, Simandle SA, Tulbert CD, Busija DW, Miller AW. Fructosefed rats are protected against ischemia/reperfusion injury. J Pharmacol Exp Ther 2003;307(3):1007-11.
• 13. Faure MJ, Rossini E, Ribuot C, Faure P. Fructose-fed rat hearts are protected against ischemia-reperfusion injury. Exp Biol Med 2006;231(4):456- 62.
• 14. Özen ST, Palabiyik O, Guksu Z, Arslan E, Tosunoğlu EA, Necdet S, et al. The effect of high-fructose feeding on hemodynamic behavior and infarct size of isolated rat hearts subjected to low-flow ischemia. Genel Tıp Dergisi 32(3):324-9.
• 15. Nandhini A, Thirunavukkarasu V, Ravichandran M, Anuradha C. Effect of taurine on biomarkers of oxidative stress in tissues of fructose-fed insulinresistant rats. Singapore Med J 2005;46(2):82.
• 16. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005;38(12):1103-11.
• 17. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by t hiobarbituric acid reaction. Anal Biochem 1979;95(2):351-8. https:// doi.org/10.1016/0003-2697(79)90738-3
• 18. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82(1):70-7. [Crossref]
• 19. Sahna E, Acet A, Kaya Ozer M, Olmez E. Myocardial ischemia-reperfusion in rats: Reduction of infarct size by either supplemental physiological or pharmacological doses of melatonin. J Pineal Res 2002;33(4):234-8.
• 20. Herman MA, Birnbaum MJ. Molecular aspects of fructose metabolism and metabolic disease. Cell Metabolism 2021;33(12):2329-54.
• 21. Parlakpinar H, Orum M, Sagir M. Pathophysiology of myocardial ischemia reperfusion injury: A review. Med Sci 2013;2(4):935-54.
• 22. Azari MH, Najafi M. Role of fructose as a potent antiarrhythmic and antiinfarct agent in isolated rat heart. Iranian J Pharm Res 2014;13(4):1303.
• 23. Busserolles J, Gueux E, Rock E, Mazur A, Rayssiguier Y. Substituting honey for refined carbohydrates protects rats from hypertriglyceridemic and prooxidative effects of fructose. J Nutr 2002;132(11):3379-82.