Deneysel İskemi/Reperfüzyon Sıçan Modelinde Eritropoietinin Böbrek Hasarindaki Rolü

Öz

Amaç: Eritroid progenitör hücrelerinin güçlü bir aktivatörü olan eritropoietin (EPO), hipoksi sırasında belirgin şekilde yükselmektedir. Arteriyer blokaj veya organ bağışının neden olabileceği renal iskemi böbrek dokusundaki hücre ölümünün başlıca sebebi olmakla birlikte reperfüzyon oluşan bu hasarı arttırmaktadır.Bu çalışmada eritropoietin uygulanmasının iskemi/reperfüzyon (I/R) sonrasında böbreklerde oluşan hasar üzerindeki etkisini araştırmayı amaçladık. Gereç ve yöntemler: Çalışmamızda 30 adet rat kullanıldı. Ratlar her grupta 6 adet olacak şekilde kontrol, EPO, iskemi, iskemi/reperfüzyon (I/R) ve I/R+EPO olmak üzere beş gruba ayrıldı. Böbrek doku örnekleri histopatolojik incelemeler için hematoksilin-eozin (H&E) ile boyandı, otofajiyi tespit edebilmek için Beclin-1 proteininin immünoekspresyonu ve apoptoz için TUNEL yöntemi kullanılarak değerlendirildi. Bulgular: Histopatolojik incelemeler sonucunda iskemi reperfüzyon grubuna ait böbrek dokusunda böbrek tübüllerinin epitelinde bozulma, glomerüler ve peritübüler kanama olduğu tespit edildi. Otofajik bir belirteç olan Beclin-1 immünekspresyonu ve TUNEL pozitif apoptotik hücrelerin I/R grubuna ait örneklerde diğer gruplara kıyasla anlamlı olarak arttığı (p < 0.05) saptandı. Bunlara ek olarak, eritropoietin verilen grupta Beclin-1 ekspresyonunda ve TUNEL pozitif hücrelerin sayısında azalma gözlendi. Sonuç: Bu çalışma ile elde ettiğimiz veriler doğrultusunda eritropoietin tedavisinin renal iskemi/reperfüzyon sonrası böbrek hasarını azaltmada ve histomorfolojik hasarı iyileştirmede etkili olabileceğini düşünmekteyiz.

Anahtar Kelimeler

• Apoptoz
• Eritropoietin
• Histolopatoloji
• Otofaji
• Renal iskemi/reperfüzyon

THE ROLE OF ERYTHROPOIETIN IN A RAT MODEL OF RENAL ISCHEMIA/ REPERFUSION INJURY

Öz

Objective: A powerful activator of erythroid progenitor cells, erythropoietin (EPO) is markedly elevated during hypoxia. A major cause of renal cell death is renal ischemia caused by artery blockage or organ transplantation, and reperfusion exacerbates the damage. The study aimed to investigate the effect of EPO treatment on renal injury following ischemia and reperfusion (I/R). Method: Thirty rats assigned to five groups of six rats each as control, EPO, ischemia, ischemia/reperfusion (I/R) and I/R+EPO.The renal tissue samples were evaluated in terms of hematoxylin-eosin (H&E) staining for histopathological changes, immunoexpression of Beclin-1 for autophagy, and the TUNEL assay for apoptosis. Results: The H&E staining showed the impairment in the tubular epithelium, glomerular and peritubular hemorrhage in the renal tissues of I/R group. Less histopathological changes were observed in I/R + EPO group. Renal tissue Beclin-1 immunoexpression and TUNEL positive cells were significantly increased in the I/R group compared with the others (p<0.05). Treatment with EPO decreased the number of the TUNEL positive cells and Beclin-1 expression. Conclusion: The data showed that EPO treatment could be effective in reducing renal injury following renal I/R and alleviate histomorphological damage.

Anahtar Kelimeler

• Apoptosis
• Autophagy
• Erythropoietin
• Histolopathology
• Renal ischemia/reperfusion

Kaynakça

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