DETERMINATION OF Cu, Fe AND Zn IN SILYBUM MARIANUM SEEDS AFTER DIGESTED USING MICROWAVE OVEN BY FAAS

Öz

This study, the concentrations of necessary trace and microelements like Cu, Fe and Zn were determined in the Silybum marianum seeds used to medicine purpose for many years. For this intention, the digestion procedure based on closed vessel-assisted microwave oven (MW) was determined a rapid, reliable, and simple digestion method for the flame atomic absorption spectrophotometric (FAAS) analysis of herbal plant. Various periods for different powers and acid mixture of the MW were examined. The obtained data show that the MW is an excellent method because it is fast and results in fewer losses and contaminants. The metal concentrations were of Fe>Cu>Zn for herbal seed, respectively.

Anahtar Kelimeler

• Silybum marianum seeds
• flame atomic absorption spectrophotometry
• microelements

SILYBUM MARIANUM TOHUMLARINDA FAAS İLE MİKRODALGA FIRIN KULLANILARAK ÇÖZÜNÜRLEŞTİRMEDEN SONRA Cu, Fe ve Zn TAYİNİ

Öz

Bu çalışma, uzun yıllardır ilaç olarak kullanılan Silybum marianum tohumlarında gerekli olan Cu, Fe ve Zn gibi eser elementlerin konsantrasyonları belirlenmiştir. Bu amaçla, tıbbi bitkinin alevli atomik absorpsiyon spektrofotometrik (FAAS) analizi için kapalı sistem destekli mikrodalga fırına (MW) dayalı çözünürleştirme prosedürü hızlı, güvenilir ve basit bir çözünürleştirme yöntemi olarak belirlenmiştir. MW'nin farklı güçleri ve asit karışımı için çeşitli periyotlar incelenmiştir. Elde edilen veriler, MW'nin hızlı olması ve daha az kayıp ve kirletici ile sonuçlanması nedeniyle mükemmel bir yöntem olduğunu göstermektedir. Tıbbi tohum için metal konsantrasyonları sırasıyla Fe>Cu>Zn idi.

Anahtar Kelimeler

• Silybum marianum tohumu
• alevli atomic absorpsiyon spektrometresi
• mikro elementler

Kaynakça

1. Referans1. WHO guidelines for assessing quality of herbal medicines with reference to contaminants and residues, World Health Organization, ISBN 978 92 4 159444 8, 2007.
2. Referans2. L.E. Feinendegen and K. Kasperek, Medical aspects of trace element research, Trace Elem. Anal. Chem Med. Biol. 1-17 (1980).
3. Referans3. W. Mertz, Trace elements in human and animal nutrition. Vol. 1-2, San Diego, CA, USA, Academic Press (1987).
4. Referans4. White, P. J., Broadley, M. R. (2009). Biofortification of crops with seven mineral elements often lacking in human diets - iron, zinc, copper, calcium, magnesium, selenium and iodine. New Phytologist, 182(1), 49–84.
5. Referans5. C.Ozcan, 2013, Kırklareli İl Merkezinde Yetişen Bazı Bitki Türlerinin Eser Element Analizleri, 3(1), 184 - 193.
6. Referans6. L. Noel, J.C. Leblanc, and T. Guerin, 2003, Determination of several elements in duplicate meals from catering establishments using closed vessel microwave digestion with inductively coupled plasma mass spectrometry detection: estimation of daily dietary intake, Food Additives & Contaminants, 20(1), 44-56.
7. Referans7. M. N. V. Prasad, Trace elements as contaminants and nutrients: Consequences in ecosystems and human health, ISBN 978-0-470-18095-2, (2008). 8. A. Çelik, A. A. Kartal, A. Akdoğan, and Y. Kaska, Determining the heavy metal pollution in Denizli (Turkey) by using Robinio pseudo-acacia L., Environment International, 31, 105 (2005).
8. Referans9. Ozturk L, Yazici MA, Yucel C, Torun A, Cekic C, Bagci A, Ozkan H, Braun H-J, Sayers Z, Cakmak I. 2006. Concentration and localization of zinc during seed development and germination in wheat. Physiologia Plantarum 128: 144–152.

9. Referans10. WHO, 1996. World Heath Organization, Geneva, Switzerland, EB109/33. (2001).
10. Referans11. M Del Río-Celestino, R Font, R Moreno-Rojas, A De Haro-Bailón, 2006, Uptake of lead and zinc by wild plants growing on contaminated soils, Industrial Crops and Products 24 (3), 230-237.
11. Referans12. Karadjova, I; Izgi, B; Gucer, S, 2002, Fractionation and speciation of Cu, Zn and Fe in wine samples by atomic absorption spectrometry, Spectrochimica Acta, Part B: Atomic Spectroscopy, 57(3), 581-590.
12. Referans13. Oliva, S.R., Raitio, H., Mingorance, M.D. (2003) Comparison of two wet digestion procedures for multi‐element analysis of plant samples. Communications in Soil Science and Plant Analysis, 34, 2913–2923.
13. Referans14. Hacisalihoglu G, Kochian LV. 2003. How do some plants tolerate low levels of soil zinc? Mechanisms of zinc efficiency in crop plants. New Phytologist, 159, 341–350.
14. Referans15. Demirezen D, Aksoy A. Heavy Metal Levels in Vegetables in Turkey are within safe limits for Cu, Zn, Ni and exceeded for Cd and Pb. J Food Qual. 2006;29:252-265..
15. Referans16. Colak Esetlili B, Pekcan T, Çobanoğlu Ö, Aydoğdu E, Turan S, Anaç D. Essential plant nutrients and heavy metals concentrations of some medicinal and aromatic plants. Journal of Agricultural Sciences. 2014;20:239-247..
16. Referans17. F.E. Smith and E.A. Arsenault, Microwave-assisted sample preparation in analytical chemistry, Talanta 43(8), 1207 (1996).
17. Referans18. I. Baranowska, K. Srogi, A. Wlochowicz, and K. Szczepanik, Determination of heavy metal contents in samples of medicinal herbs, Polish Journal of Environmental Studies, 11(5), 467 (2002).
18. Referans19. Divrikli U, Horzum N, Soylak M, Elci L. Trace heavy metal contents of some spices and herbal plants from western Anatolia, Turkey. Int J Food Sci Technol. 2006;41:712-716.