Konak Galleria mellonella Linnaeus (Lepidoptera: Pyralidae) hemolenf kimyasına indol-3-asetik asitin etkisi

Yıl 2021, Cilt: 4 Sayı: 1, 38 - 42, 30.06.2021

Fevzi Uçkan , Zülbiye Demirtürk , Hanife Merve Tetik

Öz

Oksin grubunun en önemli üyelerinden biri olan indol-3-asetik asit (IAA) bitki, mantar ve bakteriler tarafından endojen olarak üretilen bitki büyüme düzenleyicidir. Büyük balmumu güvesi Galleria mellonella, tarımda yaygın olarak kullanılan IAA’ya doğal ortamında maruz kalabilmektedir. Bu nedenle, son yıllarda model organizma olarak kullanılan G. mellonella larvalarının hemolenf kimyasındaki değişikliklere IAA’nın etkileri incelendi. G. mellonella larvalarının diyetlerine IAA’nın farklı dozları (50, 500, 1000, 5000 ve 10000 ppm) eklenerek verildi. IAA hemolenf karbohidrat, lipit ve protein seviyelerinde doza bağlı bir şekilde değişikliğe neden oldu. IAA uygulamasına bağlı olarak, larval hemolenfteki toplam glikojen ve protein miktarında bütün dozlarda azalma belirlenirken, toplam lipit miktarında ise artış gözlendi. Sonuçlarımız IAA uygulamasının böceklerde önemli fizyolojik değişikliklere neden olabileceğini göstermektedir. Bu durum pestisitler kadar bitki büyüme düzenleyicilerin de ekotoksik riskleri üzerinde gerekli çalışmalar yapılması gerektiğini göstermektedir.

Anahtar Kelimeler

Galleria mellonella, Indol-3-asetik asit, Toksisite, Hemolenf metabolitleri

Kaynakça

• [1] Uçkan F., Tüven A., Er A., Ergin E., 2008. Effects of gibberellic acid on biological parameters of the larval endoparasitoid Apanteles galleriae (Hymenoptera: Braconidae). Annals of the Entomological Society of America 101, 593-597.
• [2] Altuntaş H., 2015. Effects of Ethephon on the hemolymph metabolites of the greater wax moth Galleria mellonella L. (Lepidoptera: Pyralidae). Acta Physica Polonica A 128, 182-183.
• [3] Grunewald W., Noorden G.V., Isterdael G.V., Beeckman T., Gheysen G., Mathesius U., 2009. Manipulation of auxin transport in plant roots during Rhizobium symbiosis and nematode parasitism. The Plant Cell 21, 2553–2562.
• [4] Özyılmaz D., Özbek R., Altuntaş H., Uçkan F., 2019. Indole-3-Acetic Acid induced oxidative stress in model host Galleria mellonella L. (Lepidoptera: Pyralidae) and its endoparasitoid Pimpla turionellae (L.) (Hymenoptera: Ichneumonidae). Invertebrate Survival Journal 16 (1), 184-188.
• [5] Kaya S., Uçkan F., Er A., 2020. Influence of Indole-3-Acetic Acid on Cellular Immune Responses of Galleria mellonella L. (Lepidoptera: Pyralidae) and Pimpla turionellae L. (Hymenoptera: Ichneumonidae) in a host-parasitoid system. International Journal of Tropical Insect Science 1-11. doi:10.1007/s42690-020-00190-z.
• [6] Kulkarni N., Kushwaha D. K., Mishra V. K., Paunikar S., 2012. Effect of economical modification in artificial diet of greater wax moth Galleria mellonella (Lepidoptera: Pyralidae). Indian Journal of Entomology 74(4), 369-374.
• [7] Moamen S. A., Barakat E. M., Abokersh M. O., 2019. The biochemical alternations of Galleria mellonella hemolymph following induction of immune response. Journal of Entomology and Zoology Studies 7(4), 724-731.
• [8] John J. A., Blogg C. D., Murray F. J., Schwetz B. A., Gehring P. J., 1979. Teratogenic effects of the plant hormone indole-3-acetic acid in mice and rats. Teratology 19(3), 321–324.
• [9] Furukawa S., Abe M., Usuda K., Ogawa I., 2004. Indole-3-acetic acid induces microencephaly in rat fetuses. Toxicologic pathology 32(6), 659-667.
• [10] Bertuzzi A., Mingrone G., Gandolfi A., Greco A. V., Ringoir S., Vanholder R., 1997. Binding of indole-3-acetic acid to human serum albumin and competition with L-tryptophan. Clinica chimica acta 265(2), 183-192.
• [11] Celik I., Tuluce Y., 2006. Effects of indoleacetic acid and kinetinon lipid peroxidation and antioxidant defense in varioustissues of rats. Pesticide Biochemistry and Physiology 84, 49–54.
• [12] Celik I., Tuluce Y., 2007. Determination of toxicity of subacute treatment of some plant growth regulators on rats. Environmental Toxicology: An International Journal 22(6), 613-619.
• [13] Sak O., Uçkan F., Ergin E., 2006. Effects of cypermethrin on total body weight, glycogen, protein, and lipid contents of Pimpla turionellae (L.) (Hymenoptera: Ichneumonidae), Belgian Journal of Zoology 136, 53-58.
• [14] Van Handel E., 1985. Rapid determination of total lipids in mosquitoes. Journal of the American Mosquito Control Association 1, 302–304.
• [15] Olson D. M., Fadamiro H., Lundgren J. G., Heimpel G. E., 2000. Effects of Sugar Feeding on Carbohydrate and Lipit Metabolism in a Parasitoid Wasp. Physiological Entomology 25, 17-25.
• [16] Bradford M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein-dye binding. Analytical Biochemistry 72, 248-254.
• [17] Uçkan F., Öztürk Z., Altuntaş H., Ergin E., 2011. Effects of gibberellic acid (GA3) on biological parameters and hemolymph metabolites of the pupal endoparasitoid Pimpla turionellae (Hymenoptera: Ichneumonidae) and its host Galleria mellonella (Lepidoptera: Pyralidae). Journal of the Entomological Research Society 13(3), 1-14.
• [18] Nath B. S., Suresh A., Varma B. M., Kumar R. P. S., 1997. Changes in protein metabolism in hemolymph and fat body of the silkworm, Bombyx mori (Lepidoptera: Bombycidae) in response to organophosphorus insecticides toxicity. Ecotoxicology and Environmental Safety 36, 169–173.
• [19] Nath B. S., 2000. Changes in carbohydrate metabolism in hemolymph and fat body of the silkworm, Bombyx mori L. exposed to organophosphorus insecticides. Pesticide Biochemistry and Physiology 68, 1504–1515.
• [20] Ortel J., 1996. Metal-supplemented diets alter carbohydrate levels in tissue and hemolymph of gypsy moth larvae (Lymantria dispar, Lymantriidae, Lepidoptera). Environmental Toxicology and Chemistry 15, 1171–1176.
• [21] Folkes L. K., Candeias L. P., Wardman P., 1998. Towards targeted “oxidation therapy'' of cancer: peroxidase-catalyzed cytotoxicity of indole-3-acetic acids. International Journal of Radiation Oncology, Biology, Physics 42, 917-920.
• [22] Dalmazzo L. F., Santana-Lemos B. A., Jacomo R. H., Garcia A. B., Rego E. M., da Fonseca L. M., Falcao R. P., 2011. Antibody- targeted horseradish peroxidase associated with indole-3-acetic acid induces apoptosis in vitro in hematological malignancies. Leukemia Research 35, 657–662.
• [23] Bloemen L. J., Mandel J. S., Bond G. G., Pollock A. F., Vitek R. P., Cook R. R., 1993. An update of mortality among chemical workers potentially exposed to the herbicide 2,4-dichlorophenoxyacetic acid and its derivatives. Journal of Occupational Medicine 35, 1208-1212.