A Preliminary Study of A Lactic Acid Biosensor for the Early Detection of Dental Caries

Umut Kökbaş

doi:10.58241/ksbd.6

A Preliminary Study of A Lactic Acid Biosensor for the Early Detection of Dental Caries

Öz

Abstract Statement of Problem: Many mechanisms in the formation of dental caries, but the common point of these mechanisms is the proliferation of microorganisms in the mouth and mostly on the tooth surface. Objective: Although there are approximately 200 to 300 different microorganisms in human dental plaque, Neisseria spp. and S. mutans. The common point of these microorganisms is that they produce lactic acid. In this study, it is aimed to use a biosensor, which is a bioelectrochemical measurement system that combines the measurement capability of physical analysis systems and the specificity of biochemical systems.Material-Method: In this study, lactate oxidase enzyme was immobilised on the gold working electrode surface by means of bovine serum albumin and gelatine. The enzyme was arrested on the electrode surface with the aid of the cross-linking agent glutaraldehyde. Cyclic voltammetry current scanning was performed in lactic acid biosensor preliminary studies. Optimisation studies were conducted in the current range obtained from the peak in the cyclic voltammogram. Within the scope of the optimisation studies, optimum operating temperature and optimum operating pH of the biosensor were tested.Results: An anodic peak at +0.2 V potential was obtained in the cyclic voltammogram drawn according to the reaction on the working electrode surface. In the optimisation studies conducted, it was found that the optimum operating conditions of the biosensor were at 35 °C and pH 6.5.Conclusion: According to the data obtained from the pre-study of the bioelectrochemical method, the concentration of lactic acid in the solution can be determined. With the completion of further studies, a portable lactic acid biosensor can be made. As a result, as a result of the preliminary study, the lactic acid biosensors designed to determine in vitro lactic acid for the early diagnosis of dental caries in the future is promising.

Anahtar Kelimeler

Kaynakça

Assiri, S. A., El Meligy, O., Alzain, I. O., & Bamashmous, N. O. (2022). Assessment of dental caries and salivary characteristics among type 1 diabetic Saudi children. J Dent Sci,17(4), 1634-1639. https://doi.org/10.1016/j.jds.2022.03.010
Benn, A. M. L., Heng, N. C. K., Thomson, W. M., & Broadbent, J. M. (2022). Plaque and dental caries risk in mid-life. Caries Res. https://doi.org/10.1159/000527255
Borg-Bartolo, R., Roccuzzo, A., Molinero-Mourelle, P., Schimmel, M., Gambetta-Tessini, K., Chaurasia, A., Koca-Unsal, R. B., Tennert, C., Giacaman, R., & Campus, G. (2022). Global prevalence of edentulism and dental caries in middle-aged and elderly persons: A systematic review and meta-analysis. J Dent,127,104335. https://doi.org/10.1016/j.jdent.2022.104335
Chaudhry, S., Haroon, F., Irfan Waheed, K. A., Victor, G., Shahzad, M., & Fatima, B. (2022). Blood Lactate Levels And Lactate Clearance As Predictors Of Mortality In Neonatal Sepsis. J Ayub Med Coll Abbottabad,34(3), 438-441. https://doi.org/10.55519/JAMC-03-9087
Fernandez-Bonet, J., Marichalar-Mendia, X., & Lertxundi-Manterola, A. (2022). Childhood dental caries experience in northern Spain: a cross-sectional study. Eur Arch Paediatr Dent. https://doi.org/10.1007/s40368-022-00762-2
Folayan, M. O., Adeniyi, A. A., Arowolo, O., Maureen, C. N., Alade, M. A., & Tantawi, M. E. (2022). Risk indicators for dental caries, and gingivitis among 6-11-year-old children in Nigeria: a household-based survey. BMC Oral Health,22(1), 465. https://doi.org/10.1186/s12903-022-02470-1
Garcia-Rey, S., Ojeda, E., Gunatilake, U. B., Basabe-Desmonts, L., & Benito-Lopez, F. (2021). Alginate Bead Biosystem for the Determination of Lactate in Sweat Using Image Analysis. Biosensors (Basel),11(10). https://doi.org/10.3390/bios11100379
Goode, J. A., Rushworth, J. V., & Millner, P. A. (2015). Biosensor Regeneration: A Review of Common Techniques and Outcomes. Langmuir,31(23), 6267-6276. https://doi.org/10.1021/la503533g

Hamilton, S., Shea, D., Ibsen, S., & Brasino, M. (2022). On-chip dielectrophoretic recovery and detection of a lactate sensing probiotic from model human saliva. Electrophoresis. https://doi.org/10.1002/elps.202200214
Kaur, H., Kumar, R., Babu, J. N., & Mittal, S. (2015). Advances in arsenic biosensor development--a comprehensive review. Biosens Bioelectron,63, 533-545. https://doi.org/10.1016/j.bios.2014.08.003
Kimmie-Dhansay, F., & Bhayat, A. (2022). Prevalence of dental caries in the permanent dentition amongst 12-year-olds in Africa: a systematic review and meta-analysis. BMC Oral Health,22(1), 453. https://doi.org/10.1186/s12903-022-02489-4
Korona-Glowniak, I., Skawinska-Bednarczyk, A., Wrobel, R., Pietrak, J., Tkacz-Ciebiera, I., Maslanko-Switala, M., Krawczyk, D., Bakiera, A., Borek, A., Malm, A., & Mielnik-Blaszczak, M. (2022). Streptococcus sobrinus as a Predominant Oral Bacteria Related to the Occurrence of Dental Caries in Polish Children at 12 Years Old. Int J Environ Res Public Health,19(22). https://doi.org/10.3390/ijerph192215005
Leonard, E. M., Weaver, F. E., & Nurse, C. A. (2022). Lactate sensing by neuroepithelial cells isolated from the gills of killifish (Fundulus heteroclitus). J Exp Biol. https://doi.org/10.1242/jeb.245088Matthews, P. (2022). Prevention of dental caries in children and young people. Nurs Child Young People. https://doi.org/10.7748/ncyp.2022.e145
1Mirzaei, F., Mirzaei, M., & Torkzadeh-Mahani, M. (2019). A hydrophobin-based-biosensor layered by an immobilized lactate dehydrogenase enzyme for electrochemical determination of pyruvate. Bioelectrochemistry,130, 107323. https://doi.org/10.1016/j.bioelechem.2019.06.008
Molina, G., Zar, M., Dougall, A., & McGrath, C. (2022). Management of dental caries lesions in patients with disabilities: Update of a systematic review. Front Oral Health,3, 980048. https://doi.org/10.3389/froh.2022.980048
Moriyama, C. M., Velasco, S. R. M., Butini, L., Abanto, J., Antunes, J. L. F., & Bonecker, M. (2022). How oral health literacy and parental behavior during the meals relate to dental caries in children. Braz Oral Res,36,e131. https://doi.org/10.1590/1807-3107bor-2022.vol36.0131
Nizami, M. Z. I., Yin, I. X., Lung, C. Y. K., Niu, J. Y., Mei, M. L., & Chu, C. H. (2022). In Vitro Studies of Graphene for Management of Dental Caries and Periodontal Disease: A Concise Review. Pharmaceutics,14(10). https://doi.org/10.3390/pharmaceutics14101997
Okolo, C. C., Oredugba, F. A., Denloye, O. O., & Adeyemo, Y. I. (2022). Dental Caries, Traumatic Dental Injuries and Gingivitis among Street-Children in Kano, Nigeria. West Afr J Med,39(10), 1040-1044. https://www.ncbi.nlm.nih.gov/pubmed/36260498
Olaetxea, I., Lopez, E., Valero, A., & Seifert, A. (2019). Determination of physiological lactate and pH by Raman spectroscopy. Annu Int Conf IEEE Eng Med Biol Soc,475-481. https://doi.org/10.1109/EMBC.2019.8856471
Tsvik, L., Steiner, B., Herzog, P., Haltrich, D., & Sutzl, L. (2022). Flavin Mononucleotide-Dependent l-Lactate Dehydrogenases: Expanding the Toolbox of Enzymes for l-Lactate Biosensors. ACS Omega,7(45), 41480-41492. https://doi.org/10.1021/acsomega.2c05257
Wei, Y., Wang, J., Dai, D., Wang, H., Zhang, M., Zhang, Z., Zhou, X., He, L., & Cheng, L. (2022). Application of a Caries Treatment Difficulty Assessment System in Dental Caries Management. Int J Environ Res Public Health,19(21). https://doi.org/10.3390/ijerph192114069
Zhang, J. S., Chu, C. H., & Yu, O. Y. (2022). Oral Microbiome and Dental Caries Development. Dent J (Basel),10(10). https://doi.org/10.3390/dj10100184
Zhang, S., Chen, Y. C., Riezk, A., Ming, D., Tsvik, L., Sutzl, L., Holmes, A., & O'Hare, D. (2022). Rapid Measurement of Lactate in the Exhaled Breath Condensate: Biosensor Optimization and In-Human Proof of Concept. ACS Sens. https://doi.org/10.1021/acssensors.2c01739
Zhou, Y., Qi, M., & Yang, M. (2022). Fluorescence determination of lactate dehydrogenase activity based on silicon quantum dots. Spectrochim Acta A Mol Biomol Spectrosc,268,120697. https://doi.org/10.1016/j.saa.2021.120697

Ayrıntılar

Birincil Dil

İngilizce

Konular

Halk Sağlığı (Diğer)

Bölüm

Araştırma Makalesi

Yazarlar

Umut Kökbaş
0000-0003-4028-3458
Türkiye

Yayımlanma Tarihi

30 Nisan 2023

Gönderilme Tarihi

8 Mart 2023

Kabul Tarihi

28 Mart 2023

Yayımlandığı Sayı

Yıl 2023 Cilt: 1 Sayı: 2

DOI

https://doi.org/10.58241/ksbd.6

IZ

https://izlik.org/JA55XL88XT

Kaynak Göster

RIS / Bibtex

APA

Kökbaş, U. (2023). A Preliminary Study of A Lactic Acid Biosensor for the Early Detection of Dental Caries. Kapadokya Sağlık Bilimleri Dergisi, 1(2), 55-63. https://doi.org/10.58241/ksbd.6

AMA

1.Kökbaş U. A Preliminary Study of A Lactic Acid Biosensor for the Early Detection of Dental Caries. KSBDER. 2023;1(2):55-63. doi:10.58241/ksbd.6

Chicago

Kökbaş, Umut. 2023. “A Preliminary Study of A Lactic Acid Biosensor for the Early Detection of Dental Caries”. Kapadokya Sağlık Bilimleri Dergisi 1 (2): 55-63. https://doi.org/10.58241/ksbd.6.

EndNote

Kökbaş U (01 Nisan 2023) A Preliminary Study of A Lactic Acid Biosensor for the Early Detection of Dental Caries. Kapadokya Sağlık Bilimleri Dergisi 1 2 55–63.

IEEE

[1]U. Kökbaş, “A Preliminary Study of A Lactic Acid Biosensor for the Early Detection of Dental Caries”, KSBDER, c. 1, sy 2, ss. 55–63, Nis. 2023, doi: 10.58241/ksbd.6.

ISNAD

Kökbaş, Umut. “A Preliminary Study of A Lactic Acid Biosensor for the Early Detection of Dental Caries”. Kapadokya Sağlık Bilimleri Dergisi 1/2 (01 Nisan 2023): 55-63. https://doi.org/10.58241/ksbd.6.

JAMA

1.Kökbaş U. A Preliminary Study of A Lactic Acid Biosensor for the Early Detection of Dental Caries. KSBDER. 2023;1:55–63.

MLA

Kökbaş, Umut. “A Preliminary Study of A Lactic Acid Biosensor for the Early Detection of Dental Caries”. Kapadokya Sağlık Bilimleri Dergisi, c. 1, sy 2, Nisan 2023, ss. 55-63, doi:10.58241/ksbd.6.

Vancouver

1.Umut Kökbaş. A Preliminary Study of A Lactic Acid Biosensor for the Early Detection of Dental Caries. KSBDER. 01 Nisan 2023;1(2):55-63. doi:10.58241/ksbd.6