A Histological and Histochemical Investigation on Oocyte Development in Gryllus bimaculatus (Orthoptera: Gryllidae)

Yıl 2016, Cilt: 19 Sayı: 3, 256 - 260, 29.10.2016

Özlem Çakıcı

Öz

This study investigated the oocyte development in the ovary of two spotted black cricket, Gryllus bimaculatus. Oocytes mature through three developmental stages. These stages include (i) previtellogenic, (ii) vitellogenic and (iii) maturation. In previtellogenic stage, oocytes are encircled by squamous follicle cells. In vitellogenic stage, yolk granules within the oocyte were firstly observed just beneath the oolemma. As vitellogenesis proceeds, yolk granules cover the whole oocyte. In addition, small sized lipid droplets were firstly seen under the yolk granules, then spreads to oocyte. This time, cubic and binucleated follicle epithelial cells encircled the oocyte. In maturation stage, oocytes are full of large yolk granules. Apart from Hematoxylin& Eosin, Periodic Acid Schiff, Bromophenol Blue and Mallory Trichrome reactions were also demonstrated. According to these three histochemical methods, yolk granules showed positive reactivity. Due to the oosorption, the resorption of oocytes, some atretic oocytes were observed. Apoptosis was also observed in atretic follicles. This study indicated that ovary type of Gryllus bimaculatus is panoistic as reported in other Gryllid species.

Key Words: Cricket, ovary, oocyte, oosorption, reproduction, vitellogenesis

 

Gryllus bimaculatus’ta (Orthoptera: Gryllidae) Oosit Gelişimi Üzerine Histolojik ve Histokimyasal Araştırma

ÖZET: Bu çalışmada iki noktalı kara çekirge, Gryllus bimaculatus türüne ait bireylerin ovaryumunda histolojik ve histokimyasal incelemeler yapılmıştır.  Oositler üç aşamada olgunlaşır, bunlar: (i) previtellojenik, (ii) vitellojenik ve (iii) olgunluk aşamalarıdır. Previtellojenik evrede oositler yassı folikül epitel hücreleri tarafından kuşatılmaktadır. Vitellojenik evrede yolk granülleri ilk olarak oolemmanın hemen altında gözlenir. Vitellogenez ilerledikçe oositin içini doldururlar. Ayrıca küçük boyutlu lipid damlaları ilkin bu yolk granüllerinin altında bulunur ve daha sonra oositi kaplar. Bu sefer kübik ve çift nukleuslu folikül epitel hücreleri oositi kuşatır. Olgunluk evresinde oositler iri yolk granülleriyle kaplıdır. Histolojik preparatlar Hematoksilen Eosin boyaması dışında Periodic Acid Schiff, Bromophenol Blue ve Mallory Trichrome ile boyanarak incelenmiştir. Bu üç histokimyasal yöntemle yolk granülleri pozitif reaksiyon göstermiştir. Oositlerin resorpsiyonu anlamındaki oosit oosorpsiyonu sebebiyle bazı atretik oositler gözlenmiştir. Ayrıca, apoptozis bu foliküllerde belirlenmiştir. Bu çalışma Gryllus bimaculatus ovaryum tipinin diğer Gryllid türlerinde bildirildiği gibi panoistik tipte olduğunu göstermiştir.

Anahtar Kelimeler: Çekirge, ovaryum, oosit, üreme, vitellogenez

Kaynakça

• Bradley, J.T., Kloc, M., Wolfe, K.G., Estridge, B.H., Bilinski, S.M. 2000. Balbiani bodies in cricket oocytes: Development, ultrastructure, and presence of localized RNAs. Differentiation 67: 117–127.
• Bruce, C.D., Colleen, M.Q. 1995. Ultrastructure of the vitellogenic egg chambers of the caddisfly Brachycentrus incanus (Insecta: Trichoptera). Invertabr Biol. 114(4): 334-343.
• Chou, M.Y., Mau, R.F.L., Jang, E.B., Vargas, R.I., Pinero, C.I. 2012. Morphological features of the ovaries during oogenesis of the oriental fruit fly, Bactrocera dorsalis, in relation to the physiological state. J Insect Sci. 12(144): 1-12.
• Czarniewska, E., Rosinski, G., Gabala, E., Kuczer, M. 2014. The natural insect peptide Neb-colloostatin induces ovarian atresia and apoptosis in the mealworm Tenebrio molitor. BMC Dev Biol. 14: 1-10.
• De Oliveira, P.R, Mathias, M.I.C, Bechara, H.G. 2006. Amblyomma triste (Koch, 1844) (Acari: Ixodidae): Morphological description of the ovary and of vitellogenesis. Exp Parasitol. 113: 179-185.
• Fruttero, L.L., Frede, S., Rubiolo, E.R., Canavoso, L.E. 2011. The storage of nutritional resources during vitellogenesis of Panstrongylus megistus (Hemiptera: Reduviidae): The pathways of lipophorin in lipid delivery to developing oocytes. J Insect Physiol. 57: 475-486.
• Gilberto, G., Milvia, C. 1999. Oogenesis in supplementary reproductive of Reticulitermes lucifugus Rossi (Isoptera-Rhinotermitidae): an ultrastructural study. Invertebr Repr Dev. 35(1): 65-79.
• Guo, J.Y., Dong, S.Z., Ye, G.Y., Li, K., Zhu, J.Y., Fang, Q., Hu, C. 2011. Oosorption in endoparasitoid, Pteromalus puparum. J Insect Sci. 11: 1-11.
• Jung-Kong, K., Joon, J.G. 1999. Ovarian structure in the earwing, Forficula auricularia (Dermaptera: Forficulidae). Korean J Entomol. 29(1): 55-63.
• Kanapadinchareveetil, S., Chandrasekhar, L., Gopi, J., Lenka, D.R., Vasu, A., KGopalan, A.K., Nair, S.N., Ravindran, R., Juliet, S., Ghosh, S. 2015. Histoarchitecture of the ovary of Rhipicephalus (Boophilus)annulatus during pre-and postengorgementperiod. Scientific World Journal doi: 10.1155/2015/126584.
• Kotaki, T. 2005. Oosorption in the stink bug, Plautia crossota stali: Follicle cells as the site of protein degradation. Invertebr Repr Develop 47: 147-153.
• Kotaki, T. 2006. The fate of vitellogenin and site of protein degradation during oosorptionin the Brown-winged green bug, Plautia crossota stali. Formosan Entomol 26: 99-108.
• Lemos, W.P., Zanuncio, V.V., Ramalho, F.S., Zanuncio, J.C., Serrao, J.E. 2010. Ovary Histology of the Predator Brontocoris tabidus (Hemiptera: Pentatomidae) of Two Ages Fed on Different Diets. Entomol News. 121: 230-235.
• Lodos, N. 1975. Türkiye Entomolojisi genel, uygulamalı ve faunistik (Ders kitabı). Ege Üniversitesi Ziraat Fakültesi Yayınları No: 282 Bornova-İzmir
• Ma, W.K., Ramaswamy, S.B. 1990. Histochemistry of yolk formation in the ovaries of the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois) (Hemiptera: Miridae). Zool Sci. 7: 147-151.
• Mohamed, M.I., Khaled, A.S., Abdel Fattah, H.M., Hussein, M.A., Salem, D.A.M., Fawki, S. 2015. Ultrastructure and histopathological alteration in the ovaries of Callosobruchus maculatus (F.) (Coleoptera, Chrysomelidae) induced by the solar radiation. J Basic Appl Zool. 68: 19-32.
• Nation, J.L. 2015. Insect Physiology and Biochemistry. Third Edition. CRC Press, Taylor&Francis Group. 690 p.
• Postlethwait, J.H., Giorgi, F. 1985. Vitellogenesis in insects. In: Browder, W.L. (Ed.), Developmental Biology: A Comprehensive Synthesis, vol. 1. Plenum Press, New York, pp. 85–126.
• Snigirevskaya, E.S., Raikhel, A.S. 2005. Receptor-mediated endocytosis of yolk proteins in insect oocytes. In: Raikheil, A.S. (Ed.), Reproductive Biology of Invertebrates. Progress in Vitellogenesis, vol. XII, part B. Science Publishers Inc., pp. 199-227.
• Stepanova, I.S., Bogolyubov, D.S., Parfenov, V.N. 2007. Cajal bodies in insects. II. Molecular composition of cajal bodies in oocytes of house cricket. Relationship between cajal bodies and interchromatin granüle clusters. Cell Tissue Biol. 1(1): 14-29.
• Valle, D. 1993. Vitellogenesis in insects and other groups-A Review. Mem Inst Oswaldo Cruz 88(1): 1-26.
• Wigglesworth, V.B. 1974. Reproduction in: Insect Physiology. Chapman & Hall, London. pp. 96-113.
• Zeng, V., Ewen-Campen, B., Horch, H.W., Roth, S., Mito, T., Extavour, C.G. 2013. Developmental gene discovery in a hemimetabolous insect: De Novo Assembley and annotation of a transcriptome for the cricket Gryllus bimaculatus. Plos One 8(5): e61479 doi:10.1371/journal.pone.0061479.