Primula vulgaris Yaprak Ekstraktının Antioksidan ve Sitotoksik Özelliklerinin Değerlendirilmesi

Yıl 2017, Cilt: 20 Sayı: 4, 361 - 367, 10.04.2017

Selim Demir , İbrahim Turan , Rezzan Aliyazıcıoğlu , Yüksel Aliyazıcıoğlu

https://doi.org/10.18016/ksudobil.288175

Cited By: 2

Öz

Primula vulgaris, Primula cinsine mensup bir bitki olup, bu bitkiler sıklıkla
geleneksel tedavide kullanılmaktadırlar. Farklı Primula türlerinin farklı ekstraktlarının antioksidan ve sitotoksik
etkilerini konu alan çok sayıda çalışma olmasına rağmen, P. vulgaris ekstraktlarının bu özellikleri ile ilgili sınırlı
sayıda çalışma bulunmaktadır. Bu çalışmanın amacı P. vulgaris yapraklarının dimetil sülfoksitli ekstraktının
antioksidan özelliklerinin ve sitotoksik etkilerinin belirlenmesidir.
Ekstraktın toplam fenolik madde miktarı, toplam flavonoid madde miktarı ve
indirgeyici antioksidan güç tayini spektrofotometrik yöntemler kullanılarak
belirlendi. Ekstraktın sitotoksik etkisi ise insan akciğer (A549), karaciğer
(HepG2), meme (MCF-7), prostat (PC-3) ve kolon (WiDr) kanser hücre serileri ile
normal insan fibroblast hücre serisi üzerinde MTT yöntemi kullanılarak
değerlendirildi. Ekstraktın toplam fenolik madde içeriği ve indirgeyici
antioksidan güç değerleri 100 g örnek başına sırasıyla 1890.1±11.2 mg gallik
asit eşdeğeri ve 3233.1±176.2 mg troloks eşdeğeri olarak bulundu. Ekstrakt
çalışılan tüm kanser hücre serileri üzerinde normal fibroblast hücrelerine göre
seçici sitotoksik etki gösterdi ve IC₅₀ değerleri 133.3-253.8 µg/mL
arasında hesaplandı. Bu çalışma P.
vulgaris yaprak ekstraktının antioksidan özelliklerini ve kanser hücre
serileri üzerindeki sitotoksik etkisini ortaya koyan ilk çalışmadır. Ekstraktta
bulunan antioksidan ve sitotoksik etki gösteren molekülleri ve bu etkilerin
mekanizmalarını belirleyebilmek için daha ileri çalışmalar gerekmektedir.

Anahtar Kelimeler

Antioksidan aktivite, Kanser, Primula vulgaris, Sitotoksisite

Evaluation of Antioxidant and Cytotoxic Properties of Primula vulgaris Leaf Extract

Öz

Primula vulgaris belongs to the genus Primula and this genus is frequently used in traditional medicine.
Numerous studies reported the antioxidant and cytotoxic effects of various
extracts of different Primula species
but, there were limited studies of the antioxidant and cytotoxic effect of P. vulgaris. The aim of this study was
to determine the antioxidant properties and cytotoxic effects of dimethyl
sulfoxide extract of leaves of P.
vulgaris. The total polyphenolic content (TPC), total flavonoid content
(TFC) and reducing antioxidant power of extract were evaluated using
spectrophotometric procedures. The cytotoxic effects of extract were revealed
on human lung (A549), liver (HepG2), breast (MCF-7), prostate (PC-3), and colon
(WiDr) cancer cell lines and normal fibroblast cells using thiazolyl blue tetrazolium
bromide (MTT) assay. TPC and FRAP values were 1890.1±11.2 mg gallic acid
equivalents and 3233.1±176.2 mg trolox equivalents per 100 g sample,
respectively. The extract showed selective cytotoxic effects on all studied
cancer cell lines compared to normal fibroblast cells and IC₅₀
values ranged from 133.3-253.8 µg/mL. This is the first study to reveal the
antioxidant properties of P. vulgaris
leaf extract and its cytotoxic effect on cancer cell lines. Further studies are
now needed to identify the antioxidant and cytotoxic molecules in the extract
and their mechanisms.

Anahtar Kelimeler

Antioxidant activity, Cancer, Cytotoxicity, Primula vulgaris

Kaynakça

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