Antimicrobial and Antioxidant Activity of Echinops phaeocephalus (Asteraceae)

Yıl 2017, Cilt: 20 Sayı: 4, 355 - 360, 01.08.2017

Handan Şapcı Cem Vural

https://doi.org/10.18016/ksudobil.288299

Cited By: 1

Öz

The
aim of this study was to investigate antimicrobial and antioxidant activity of Echinops phaeocephalus extracts prepared with different solvents. For antioxidant potential
evaluation, extraction of E. phaeocephalus was obtained by three
solvents of different polarities including hexane, methanol and chloroform.
Antioxidant potential was evaluated by estimating DPPH radical scavenging
activity, total phenolic activity and total flavonoid activity assay.
Antimicrobial activity was determined by minimal inhibitory concentration (MIC)
and disc diffusion tests. Efficiency of different solvents was compared for the
yield of antioxidant extracts from samples. The study results indicated that, E. phaeocephalus could be an important
natural antioxidant. The evaluations of solvents for effect of extraction resulted
that methanol was more effective than other solvents. Echinops phaeocephalus derived extracts were observed to have varies
effects on microorganisms. The results of the antioxidant activity assay
indicated that the highest phenolic content was in the extract of hexane, while
the highest flavonoid content was in methanol extract. Especially methanol and
hexane extracts showed high efficiency according to DPPH radical scavenging activity results. Our results
concluded that E. phaeocephalus sustains
an important antioxidant and antimicrobial activity.

Anahtar Kelimeler

Compositae, phenolic content, flavonoid, antibacterial, antifungal

Echinops phaeocephalus (Asteraceae) Türünün Antimikrobiyal ve Antioksidan Aktivitesi

Öz

Bu çalışmada, E. phaeocephalus türünün
antimikrobiyal ve antioksidan aktivitesini araştırmak amaçlanmıştır. E. phaeocephalus türünün
ekstraksiyonları hekzan, methanol ve kloroform olmak üzere üç farklı çözücü
kullanılarak elde edilmiştir. Antioksidan kapasitesi DPPH radikali süpürücü
aktivite tayini, toplam fenolik içeriğin belirlenmesi ve toplam flavanoid
içeriğinin belirlenmesi testleri yapılarak belirlenmiştir. Antimikrobiyal
aktiviteleri disk difüzyon testi ve mikrodilüsyon (MIC) yöntemi kullanılarak
araştırılmıştır. Çözücüler ekstraksiyon
verimi bakımından değerlendirildiğinde metanolün diğer çözücülerden daha etkili
olduğu tespit edilmiştir. Echinops
phaeocephalus türü ekstraktlarının mikroorganizmalar üzerinde değişen
etkilere sahip olduğu gözlenmiştir. Antioksidan aktivite
tayini testleri neticesinde, fenolik madde miktarı en yüksek hekzan ile elde
edilen ekstraktda gözlenirken, flavanoid madde içeriği bakımından zengin olan
ekstrakt metanol ile elde edilen ekstrakt olmuştur. DPPH radikali süpürücü
aktivite tayini sonuçlarında ise özellikle metanol ve hekzan ekstraktlarından
yüksek verim elde edilmiştir. Elde edilen sonuçlar neticesinde de E. phaeocephalus türünün önemli derecede
antioksidan ve antimikrobiyal aktiviteye sahip olduğu belirlenmiştir.

Anahtar Kelimeler

fenolik içerik, Compositae, flavanoid, antibakterial, antifungal

Kaynakça

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