Effects of Shilajit Added to Honeybee Sperm Extender on Sperm Freezing

Yıl 2022, Cilt: 25 Sayı: Ek Sayı 2, 591 - 596, 30.12.2022

Arda Onur Özkök Burcu Yalçın Murat Selcuk

https://doi.org/10.18016/ksutarimdoga.vi.1031295

Cited By: 1

Öz

The aim of this study was to determine the effects of shilajit (S) at different doses (0, 5, 10, 15, 20%) added to honey bee (Apis melilifera) semen extender on motility, plasma membrane integrity and acrosome integrity parameters. In the study, a total of 25 colonies, 5 groups and 5 colonies in each group, were used. The control group (S-0) constitutes the group where shilajit was not added to the honeybee extender. S-1, S-2, S-3 and S-4 groups were formed as groups with 5, 10, 15 and 20% shilajit added to the honey bee extender, respectively. Sperm were collected from approximately 400 drones aged 14-21 days in approximately 25 colonies that were fed every other day. Then they were frozen in liquid nitrogen vapor and stored in liquid nitrogen at -196°C for approximately 7 months. At the end of this period, the frozen samples were thawed at 37°C and examined in terms of relevant parameters. Compared with other groups, S-3 and S-4 groups increased sperm motility (p<0.05); It was determined that S-2, S-3 and S-4 groups increased plasma membrane integrity while the acrosome integrity was significantly preserved in the groups with different doses of shilajit added to the diluent compared to the control group (p<0.01). As a result, it was concluded that different doses of shilajit to honey bee extender had positive effects on spermatological parameters.

Anahtar Kelimeler

Honeybee, Freezing, Shilajit, Sperma

Bal Arısı Sperma Sulandırıcısına Katılan Shilajitin Spermanın Dondurulması Üzerine Etkileri

Öz

Bu çalışmanın amacı, bal arısı (Apis melifera) sperma sulandırıcısına katılan farklı dozlardaki (%0, 5, 10, 15, 20) shilajitin (S) motilite, plazma membran bütünlüğü ve akrozom bütünlüğü parametreleri üzerine etkilerini belirlemektir. Çalışmada, 5 grup ve her bir grupta 5’er adet koloni olmak üzere toplam 25 adet koloni kullanıldı. Kontrol grubu (S-0), bal arısı sulandırıcısına shilajit ilavesi yapılmayan grubu oluşturmaktadır. S-1, S-2, S-3 ve S-4 grupları ise bal arısı sulandırıcısına sırasıyla %5, 10, 15 ve 20 shilajit ilaveli gruplar olarak oluşturuldu. Gün aşırı beslenen yaklaşık 25 adet koloni içerisinde 14-21 günlük yaşta bulunan yaklaşık 400 erkek arıdan sperma toplandı ve sıvı azot buharında dondurularak, -196°C sıvı azot içerisinde yaklaşık 7 ay süre ile saklandı. Bu süre sonunda dondurulan numuneler 37°C’ de çözdürülerek ilgili parametreler yönünden incelendi. Diğer gruplar ile karşılaştırıldığında, S-3 ve S-4 gruplarının sperma motilite değerini arttırdığı (p<0.05); S-2, S-3 ve S-4 gruplarının plazma membran bütünlüğünü arttırdığı tespit edildi. Sulandırıcıya farklı dozlarda shilajit ilave edilen gruplarda, kontrol grubuna kıyasla akrozom bütünlüğününün önemli derecede korunduğu belirlendi (p<0.01). Sonuç olarak, bal arısı sulandırıcısına farklı dozlardaki shilajitin spermatolojik parametreler üzerine olumlu etkileri olduğu saptandı.

Anahtar Kelimeler

Bal arısı, Dondurma, Shilajit, Sperma

Kaynakça

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