Yıl 2019, Cilt 14 , Sayı 2, Sayfalar 75 - 79 2019-07-15

Deneysel Beyin İskemisinde Tacrolimusun Endotelin-1, Melatonin ve Heat Shock Protein-70 Üzerine Etkileri
Effects of Tacrolimus on Endothelin-1, Melatonin and Heat Shock Protein Levels in Experimental Brain Ischemia

Özlem GÜLER [1] , Mustafa YILDIZ [2] , Mehmet Nuri BOZDEMİR [3] , Ahmet KAVAKLI [4] , Adile Ferda DAĞLI [5] , Bilal ÜSTÜNDAĞ [6]


Amaç: Bu çalışmada deneysel iskemik inmede tacrolimusun plazma endotelin-1, melatonin ve beyin Hsp-70 seviyeleri üzerine etkilerini araştırmayı amaçladık.

Gereç ve Yöntem: Yirmi bir erkek Wistar-Albino rat her birinde yedi rat olan 3 grupa rasgele ayrıldı. Grup 2 ve grup 3'deki hayvanların ana karotis arterleri anestezi altında 10 dakika süresince anevrizma klempleri ile bağlandı. Grup 1'deki hayvanlar bağlanmadı ve herhangi bir tedavi almadı. Grup 2'deki ratlara 1 ml salin ve grup 3'dekilere 1 mg/kg tacrolimus intraperitonal olarak verildi. Enjeksiyonlar iskemiden 1 saat önce ve iskemiden 6,24 ve 48 ve 72 saat sonra uygulandı. Tüm hayvanların 4. günde kafaları kesildi, plazmaları ve beyinleri alındı. Plazma endotelin-1 ve melatonin seviyeleri ölçüldü. Beyin Hsp-70 immünboyanması ve nöron hücre ölümü yarı nicel olarak ölçüldü.

Bulgular: Plazma endotelin-1 seviyeleri grup 3'de grup 2'den daha yüksekti, ancak grup 1 ve grup 2'de benzerdi. Grup 1 deki plazma melatonin seviyeleri grup 2 ve grup 3'den daha düşüktü. Grup 2'deki melatonin seviyeleri grup 3'den daha yüksekti. Grup 3'deki hücre ölümü grup 2'den daha düşüktü. Grup 2'deki ortalama Hsp-70 immün boyanma yoğunluğu grup 3 ve grup 1'den daha fazlaydı. Grup 1'deki ortalama Hsp-70 immün boyanma yoğunluğu grup 3'den daha düşüktü.

Sonuç: İskemik inmede tacrolimus uygulanması plazma melatonin ve beyin Hsp-70 seviyelerini azaltır, plazma endotelin-1 seviyelerini arttırır ve nöroprotektif etki gösterir.

Objective: We aimed to investigate the effects of tacrolimus on plasma endothelin-1, melatonin and brain Hsp-70 levels in experimental ischemic stroke in this study.

Material and Methods: Twenty-one male Wistar-Albino rats randomly divided into three groups which included seven rats. Animals in group 2 and group 3 were anesthetized and bilateral common carotid arteries were clamped with aneurysm clips for 10 minutes. Animals in group 1 were not clamped and were not given any treatment. Rats in group 2 were received 1 ml saline and in group 3 were received 1 mg/kg tacrolimus intraperitoneally. Injections were applied 1st hour before ischemia and at 6th, 24th, 48th and 72nd hours post ischemia. All the animals were decapitated on the 4th day and plasmas were obtained and brains were excised. Plasma endothelin-1 and melatonin levels were measured. Brain Hsp-70 immunostaining and neuron cell death were scored semiquantitatively.

Results: The plasma endothelin-1 levels in group 3 were higher than group 2 and group 1, but were similar in group 1 and group 2.  In group 1 plasma melatonin levels were lesser than group 2 and group 3. In group 2 plasma melatonin levels were higher than group 3. The mean neuron death in group 3 was lesser than in group 2. The mean Hsp-70 immunostaining intensity in group 2 was greater than group 3 and group 1. In group 1 the mean Hsp-70 immünostaining intensity was lesser than group 3.

Conclusions: Tacrolimus administration in ischemic stroke reduces plasma melatonin and brain Hsp-70 levels and increases plasma endothelin-1 levels and has neuroprotective effect.

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Birincil Dil en
Konular Sağlık Bilimleri ve Hizmetleri
Bölüm Araştırma Makaleleri
Yazarlar

Orcid: 0000-0002-1444-7730
Yazar: Özlem GÜLER (Sorumlu Yazar)
Kurum: KAHRAMANMARAŞ SÜTÇÜ İMAM ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Orcid: 0000-0000-0000-0000
Yazar: Mustafa YILDIZ
Kurum: FIRAT ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Orcid: 0000-0000-0000-0000
Yazar: Mehmet Nuri BOZDEMİR
Kurum: FIRAT ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Orcid: 0000-0000-0000-0000
Yazar: Ahmet KAVAKLI
Kurum: FIRAT ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Orcid: 0000-0000-0000-0000
Yazar: Adile Ferda DAĞLI
Kurum: FIRAT ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Orcid: 0000-0000-0000-0000
Yazar: Bilal ÜSTÜNDAĞ
Kurum: FIRAT ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Tarihler

Yayımlanma Tarihi : 15 Temmuz 2019

Bibtex @araştırma makalesi { ksutfd533419, journal = {Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi}, issn = {1303-6610}, eissn = {2547-9598}, address = {}, publisher = {Kahramanmaraş Sütçü İmam Üniversitesi}, year = {2019}, volume = {14}, pages = {75 - 79}, doi = {10.17517/ksutfd.533419}, title = {Effects of Tacrolimus on Endothelin-1, Melatonin and Heat Shock Protein Levels in Experimental Brain Ischemia}, key = {cite}, author = {GÜLER, Özlem and YILDIZ, Mustafa and BOZDEMİR, Mehmet Nuri and KAVAKLI, Ahmet and DAĞLI, Adile Ferda and ÜSTÜNDAĞ, Bilal} }
APA GÜLER, Ö , YILDIZ, M , BOZDEMİR, M , KAVAKLI, A , DAĞLI, A , ÜSTÜNDAĞ, B . (2019). Effects of Tacrolimus on Endothelin-1, Melatonin and Heat Shock Protein Levels in Experimental Brain Ischemia. Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi , 14 (2) , 75-79 . DOI: 10.17517/ksutfd.533419
MLA GÜLER, Ö , YILDIZ, M , BOZDEMİR, M , KAVAKLI, A , DAĞLI, A , ÜSTÜNDAĞ, B . "Effects of Tacrolimus on Endothelin-1, Melatonin and Heat Shock Protein Levels in Experimental Brain Ischemia". Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi 14 (2019 ): 75-79 <https://dergipark.org.tr/tr/pub/ksutfd/issue/45521/533419>
Chicago GÜLER, Ö , YILDIZ, M , BOZDEMİR, M , KAVAKLI, A , DAĞLI, A , ÜSTÜNDAĞ, B . "Effects of Tacrolimus on Endothelin-1, Melatonin and Heat Shock Protein Levels in Experimental Brain Ischemia". Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi 14 (2019 ): 75-79
RIS TY - JOUR T1 - Effects of Tacrolimus on Endothelin-1, Melatonin and Heat Shock Protein Levels in Experimental Brain Ischemia AU - Özlem GÜLER , Mustafa YILDIZ , Mehmet Nuri BOZDEMİR , Ahmet KAVAKLI , Adile Ferda DAĞLI , Bilal ÜSTÜNDAĞ Y1 - 2019 PY - 2019 N1 - doi: 10.17517/ksutfd.533419 DO - 10.17517/ksutfd.533419 T2 - Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi JF - Journal JO - JOR SP - 75 EP - 79 VL - 14 IS - 2 SN - 1303-6610-2547-9598 M3 - doi: 10.17517/ksutfd.533419 UR - https://doi.org/10.17517/ksutfd.533419 Y2 - 2019 ER -
EndNote %0 Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi Effects of Tacrolimus on Endothelin-1, Melatonin and Heat Shock Protein Levels in Experimental Brain Ischemia %A Özlem GÜLER , Mustafa YILDIZ , Mehmet Nuri BOZDEMİR , Ahmet KAVAKLI , Adile Ferda DAĞLI , Bilal ÜSTÜNDAĞ %T Effects of Tacrolimus on Endothelin-1, Melatonin and Heat Shock Protein Levels in Experimental Brain Ischemia %D 2019 %J Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi %P 1303-6610-2547-9598 %V 14 %N 2 %R doi: 10.17517/ksutfd.533419 %U 10.17517/ksutfd.533419
ISNAD GÜLER, Özlem , YILDIZ, Mustafa , BOZDEMİR, Mehmet Nuri , KAVAKLI, Ahmet , DAĞLI, Adile Ferda , ÜSTÜNDAĞ, Bilal . "Effects of Tacrolimus on Endothelin-1, Melatonin and Heat Shock Protein Levels in Experimental Brain Ischemia". Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi 14 / 2 (Temmuz 2019): 75-79 . https://doi.org/10.17517/ksutfd.533419
AMA GÜLER Ö , YILDIZ M , BOZDEMİR M , KAVAKLI A , DAĞLI A , ÜSTÜNDAĞ B . Effects of Tacrolimus on Endothelin-1, Melatonin and Heat Shock Protein Levels in Experimental Brain Ischemia. KSÜ Tıp Fak Der. 2019; 14(2): 75-79.
Vancouver GÜLER Ö , YILDIZ M , BOZDEMİR M , KAVAKLI A , DAĞLI A , ÜSTÜNDAĞ B . Effects of Tacrolimus on Endothelin-1, Melatonin and Heat Shock Protein Levels in Experimental Brain Ischemia. Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi. 2019; 14(2): 79-75.