Histological and Biochemical Investigation of the Effects of Low Intensity Pulsed Ultrasound on Orthodontic Tooth Movement

Year 2019, , 119 - 125, 25.03.2019

Mine Geçgelen Cesur , Tuna Onal Mehmet Dincer Bilgin Fevziye Burcu Sirin Sevinc Inan , Ergun Cem Koken , Afra Alkan , Gokhan Cesur

https://doi.org/10.18521/ktd.510808

Cited By: 3

Abstract

Objective: The aim of this study is to investigate the
effects of low intensity pulsed ultrasound on orthodontic tooth movement in
rats.

Methods:
For this study,
40 12-week-old adult male Wistar albino rats from the Animal Laboratory at
Adnan Menderes University, Faculty of Medicine, were used. Rats were divided into four groups of ten. Group 1 was
the untreated control group. In group 2, an orthodontic spring was used to move
teeth. In groups 3 and 4, orthodontic treatment was combined with low intensity pulsed ultrasound at 16
J/cm² or 48 J/cm², respectively, for 14 days. Tooth
movement was measured on day 14. Serum bone alkaline phosphatase (BALP) and
C-telopeptide of type I collagen (CTX-I) levels were analyzed biochemically.
The number of osteoclasts, osteoblasts and inflammatory cells, capillary
density and new bone formation was determined histologically. Receptor activator
of nuclear factor-kappa B ligand (RANKL), osteoprotegerin (OPG), vascular
endothelial growth factor (VGEF) and transforming growth factor-β (TGF-β) were
assessed using immunohistochemical staining.

Results:
BALP and CTX-I levels in group 4 were significantly higher than for group 1.
Tooth movement and the number of osteoclasts, inflammatory cells, and capillary
density in group 4 were significantly greater than for group 2. The intensity
levels of RANKL and OPG in group 4 were significantly greater than for group 2.

Conclusion: Ultrasound is noninvasive application and a
promising therapy for accelerating bone remodeling during orthodontic tooth
movement.

Keywords

Orthodontic treatment, low intensity pulsed ultrasound, tooth movement

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