Effects of Cyanide on Some Histological and Immunohistochemical Parameters of Common Carp (Cyprinus carpio)

Öz

In this study, Bcl 2 and Caspase 3 expressions and histomorphological changes were investigated in the liver, gill and skin tissues of carp (Cyprinus carpio) in which 0,1 mg/L and 0,2 mg/L concentrations of cyanide were added to their environment. It was determined that the lipid accumulation, lymphocyte infiltration, fibrosis and regeneration in the liver tissues; hyperplasia, cell aggregates and goblet cells in the skin epithelium and gill filaments of fish exposed to cyanide. As a result of the study, it was observed that Bcl-2 expressions decreased and caspase-3 expressions increased in all tissues of fish exposed to cyanide at concentrations of 0,1 mg/L and 0,2 mg/L. Changes in Bcl-2 and caspase-3 expression levels result in disruption of the apoptosis mechanism in the liver, gill and skin tissues. At the end of the study, it was concluded that the examined parameters were a good indicator for cyanide intoxication.

Anahtar Kelimeler

• Cyprinus carpio
• cyanide
• histology

Proje Numarası

2015-80

Sazan Balığında (Cyprinus carpio) Bazı Histolojik ve İmmunhistokimyasal Parametreleri Üzerine Siyanürün Etkileri

Öz

Bu çalışmada bulundukları ortama 0,1 mg/L ve 0,2 mg/L konsantrasyonda siyanür eklenen sazan balıklarının (Cyprinus carpio) karaciğer, solungaç ve deri dokusu Bcl-2 ve kaspaz-3 ekspresyonları ve histomorfolojik açıdan değişimleri araştırılmıştır. Siyanüre maruz kalan balıkların karaciğer dokularında lipid birikimi, lenfosit infiltrasyonu, fibrosis ve rejenerasyon, deri epitelinde ve solungaç filamentlerinde ise hiperplazi, hücre agregatları ve goblet hücreleri tespit edilmiştir. Yapılan çalışma sonunda 0,1 mg/L ve 0,2 mg/L konsantrasyonlarda siyanüre maruz bırakılan sazan balıklarının incelenen tüm dokularında Bcl-2 ekspresyonlarının azaldığı, kaspaz-3 ekspresyonlarının ise arttığı görülmüştür. Bcl-2 ve kaspaz-3 ekspresyon düzeylerindeki değişimler ilgili dokulardaki apoptosis mekanizmasının bozulması sonucunu doğurmaktadır. Çalışma sonunda incelenen parametrelerin siyanür intoksikasyonu için iyi bir indikatör olduğu sonucuna ulaşılmıştır.

Anahtar Kelimeler

• Cyprinus carpio
• siyanür
• histoloji

Destekleyen Kurum

KÜTAHYA DUMLUPINAR ÜNİVERSİTESİ BİLİMSEL ARAŞTIRMA PROJELERİ KOORDİNATÖRLÜĞÜ

Proje Numarası

2015-80

Kaynakça

1. Al-Ghanbusi R, Ba-Omar T, Victor R. 2012. Effect of deltamethrin on the gills of Aphanius dispar: A microscopic study. Tissue and Cell 44:7-14. doi: 10.1016/j.tice.2011.09.003
2. Arockiaraj J, Palanisamy R, Arasu A, Sathyamoorthi A, Kumaresan V, Bhatt P, Chaurasia MK, Pasupuleti M, Gnanam AJ. 2015. An anti-apoptotic B-cell lymphoma-2 (BCL-2) from Channa striatus: Sequence analysis and delayed and advanced gene expression in response to fungal, bacterial and poly I:C induction. Molecular Immunology 63:586-594. doi: 10.1016/j.molimm.2014.07.018
3. Ballantyne B. 1983. The influence of exposure route and species on the acute lethal toxicity and tissue concentrations of cyanide, In: Hayes AW, Schnell RC, Miya TS, eds., Developments in the science and practice of toxicology, New York, NY: Elsevier Science Publishers, pp.583-586.
4. Ben Ameur W, Lapuente J, El Megdiche Y, Barhoumi B, Trabelsi S, Camps L, Serret J, Ramos-Lopez D, Gonzalez-Linares J, Driss MR, Borras M. 2012. Oxidative stress, genotoxicity and histopathology biomarker responses in mullet (Mugil cephalus) and sea bass (Dicentrarchus labrax) liver from Bizerte Lagoon (Tunisia). Marine Pollution Bulletin 64:241-251. doi: 10.1016/j.marpolbul.2011.11.026
5. Blaha J. 1976. Mathematical analysis of the chemical system cyanide heavy meals in water determination of components and toxicity of the system-I, The theoretical solution. Water Res 10:815-819. doi: 10.1016/0043-1354.76.90102-0
6. Cao J, Chen J, Wang J, Jia R, Xue W, Luo Y, Gan X. 2013. Effects of fluoride on liver apoptosis and Bcl-2, Bax protein expression in freshwater teleost, Cyprinus carpio. Chemosphere 91:1203-1212. doi: 10.1016/j.chemosphere.2013.01.037
7. Cengiz EI. 2006. Gill and kidney histopathology in the freshwater fish Cyprinus carpio after acute exposure to deltamethrin. Environ Toxicol Pharmacol 22:200-204. doi: 10.1016/j.etap.2006.03.006
8. David M, Kartheek RM. 2016. In vivo studies on hepato-renal impairments in freshwater fish Cyprinus carpio following exposure to sublethal concentrations of sodium cyanide. Environmental Science and Pollution Research 23:722-733. doi: 10.1007/s11356-015-5286-9

9. David M, Munaswamy V, Halappa R, Marigoudar SR. 2008. Impact of sodium cyanide on catalase activity in the freshwater exotic carp, Cyprinus carpio (Linnaeus). Pesticide Biochemistry and Physiology 92:15-18. doi: 10.1016/j.pestbp.2008.03.013
10. DiPasquale LC, Davis HV. 1971. The acute toxicity of brief exposures to hydrogen fluoride, hydrogen chloride, nitrogen dioxide, and hydrogen cyanide singly and in combination with carbon monoxide. Second Annual Conference on Environmental Toxicity 279-290.
11. Elvitigala DAS, Whang I, Premachandra HKA, Umasuthan N, Oh MJ, Jung SJ, Yeo SY, Lim BS, Lee JH, Park HC, Lee J. 2012. Caspase-3 from rock bream (Oplegnathus fasciatus): Genomic characterization and transcriptional profiling upon bacterial and viral inductions. Fish & Shellfish Immunology 33:99-110. doi: 10.1016/j.fsi.2012.04.008
12. Green DR, John CR. 1998. Mitochondria and apoptosis. Science 28;281.5381.:1309-1312 (review). doi: 10.1126/science.281.5381.1309
13. Han MC, Sağlıyan A, Polat E. 2016. Investigation of the effect of anesthetic on clove oil aquarium fish. Harran University Journal of the Faculty of Veterinary Medicine 5.1.:12 – 17.
14. Jiang WD, Liu Y, Jiang J, Wu P, Feng L, Zhou XQ. 2015. Copper exposure induces toxicity to the antioxidant system via the destruction of Nrf2/ARE signaling and caspase-3-regulated DNA damage in fish muscle: Amelioration by myo-inositol, Aquatic Toxicology 159:245–255. doi: 10.1016/j.aquatox.2014.12.020
15. Kumaresan V, Ravchandran G, Nizam F, Dhayanithi NB, Arasu MV, Al-Dhabi NA, Harikrishnan R, Arockiaraj J. 2016. Multifunctional murrel caspase 1, 2, 3, 8 and 9: Conservation, uniqueness and their pathogen-induced expression pattern. Fish & Shellfish Immunology 49:493-504. doi: 10.1016/j.fsi.2016.01.008
16. Lloyd R. 1992. Pollution and Freshwater Fish. Fishing News Book, UK.
17. Maceda-Veiga A, Monroy M, Navarro E, Viscor G, Sostoa A. 2013. Metal concentrations and pathological responses of wild native fish exposed to sewage discharge in a Mediterranean river. Science of the Total Environment 449:9-19. doi: 10.1016/j.scitotenv.2013.01.012
18. Morcillo P, Meseguer J, Esteban MA, Cuesta A. 2016. In vitro effects of metals on isolated head-kidney and blood leucocytes of the teleost fish Sparus aurata L. and Dicentrarchus labrax L. Fish & ShellfishImmunology 54:77-85. doi:10.1016/j.fsi.2016.03.164
19. Murussi CR, Costa MD, Leitemperger JW, Flores-Lopes F, Menezes CC, Loebens L, Avila LA, Rizzetti TM, Adaime MB, Zanella R, Loro VL. 2016. Acute exposure to the biopesticide azadirachtin affects parameters in the gills of common carp (Cyprinus carpio). Comparative Biochemistry and Physiology, Part C 180:49-55. doi: 10.1016/j.cbpc.2015.12.003
20. Oropesa-Jiménez AL, García-Cambero JP, Gómez-Gordo L, Roncero-Cordero V, Soler Rodríguez F. 2005. Gill modifications in the freshwater fish Cyprinus carpio after subchronic exposure to simazine. Environ. Contam. Toxicol 74:785-792. doi: 10.1007/s00128-005-0650-y
21. Poleksic V, Mitrovic-Tutundzic V. 1994. Fish gills as a monitor of sublethal and chronic effects of pollution. In: Müller, R., Lloyd, R. (Eds). Sublethal and Chronic Effects of Pollutants on Freshwater Fish, Cambridge Univ. Press, Cambridge, UK, 339-352.
22. Prashant MS. 2011. Acute toxicity, behavioral and nitrogen metabolism changes of sodium cyanide affected on tissues of Tilapia mossambica (Perters). Drug Chem Toxicol. 35.2.:178-183. doi: 10.3109/01480545.2011.589608
23. Sarı M, Akar F, Karakaş F. 1999. Determination of cyanide levels in Sorghum halepense at different vegetation stages in the Aydın Area. Turkish Journal of Veterinary and Animal Sciences 2:381-384.
24. Vidal MC, Hoole D, Williams GT. 2008.Characterisation of cDNAs of key genes involved in apoptosis in common carp (Cyprinus carpio L.). Fish & Shellfish Immunology 25:494-507. doi: 10.1016/j.fsi.2008.07.013
25. Yuan Z, Liu S, Yao J, Zeng Q, Tan S, Liu Z. 2016. Expression of Bcl-2 genes in channel catfish after bacterial infectionand hypoxia stress. Developmental and Comparative Immunology 65:79-90. doi: 10.1016/j.dci.2016.06.018