Effects of dietary soybean meal levels on reproduction parameters, the growth and gonad, gut, hepatopancreas histology of female African Cichlids Pseudotropheus socolofi

Abstract

This study was conducted to determine the effect of different soybean meal dietary supplementation levels on the growth, reproductive parameters, gonad, intestine and hepatopancreas histology of Pseudotropheus socolofi. Experimental groups were hand-fed to satiety with diets supplemented with soybean meal at levels of 3, 16, 27, 35, and 44% twice daily for 90 days. The results of this study showed that specific growth rate, weight gain, and survival rate were not affected by dietary soy proportions (P > 0.05). The worst FCR and final weight were found in those fed the diet containing 44% soy (P < 0.05). In female reproductive parameters, there was no significant difference between the groups in fertilization rate, egg production, hatching rate, egg diameter, gonadosomatic index and broodstock ovulation percentage (P > 0.05). However, due to pathological examinations in female individuals, a significant decrease was observed in the number of mature oocytes in the ovaries and goblet cells in the intestines with increasing soy levels in the diet (P < 0.05). As a result, using soybean meal up to 35% did not negatively affect growth. However, adding 44% soybean meal to diets caused histopathologically serious inflammatory reactions and decreased growth.

Keywords

• Pseudotropheus socolofi
• Soybean meal
• reproduction
• histology
• growth

Diyetteki soya küspesi oranlarının Dişi Afrika Çiklitleri Pseudotropheus socolofi’nin üreme, büyüme parametreleri, ve gonad, bağırsak, hepatopankreas histolojisi üzerine etkileri

Öz

Bu çalışma, Pseudotropheus socolofi’nin büyüme, üreme parametreleri, gonad, bağırsak ve hepatopankreas histolojisi üzerine farklı düzeylerde soya küspesi diyet takviyesinin etkisini belirlemek amacıyla yapıldı. Deney grupları, 90 gün boyunca günde iki kez %3, 16, 27, 35 ve 44 seviyelerinde soya küspesi ile desteklenen diyetlerle doyana kadar elle beslendi. Bu çalışmanın sonuçları spesifik büyüme hızının, kilo alımının ve hayatta kalma oranının diyetteki soya oranlarından etkilenmediğini gösterdi ( P> 0,05). En kötü FCR ve son ağırlık, %44 soya içeren diyetle beslenenlerde bulundu (P < 0,05). Dişi üreme parametrelerinde ise döllenme oranı, yumurta verimi, kuluçka oranı, yumurta çapı, gonadosomatik indeks ve anaç yumurtlama yüzdesinde gruplar arasında anlamlı bir fark bulunmamıştır (P > 0,05). Ancak dişi bireylerde yapılan patolojik incelemeler sonucunda diyetteki soya düzeylerinin artmasıyla birlikte yumurtalıklardaki olgun oosit sayısında ve bağırsaklardaki goblet hücrelerinde önemli bir azalma gözlendi ( P< 0,05). Sonuç olarak soya küspesinin %35'e kadar kullanılması büyümeyi olumsuz etkilememiştir. Ancak diyetlere %44 oranında soya fasulyesi küspesinin eklenmesi histopatolojik olarak ciddi inflamatuar reaksiyonlara ve büyümede azalmaya neden olmuştur.

Anahtar Kelimeler

• Pseudotropheus socolofi
• soya küspesi
• üreme
• histoloji
• büyüme

Kaynakça

1. Ahmed YM, Idowu TA, Adedeji HA, Sogbesan OA. 2015. Sex Reversal and Growth Performances in Clarias gariepinus (Burchell, 1822) Fry Fed Dietary Genistein under Laboratory Conditions. J. Agric. Food. Tech., 5(5)1-4
2. Bagheri T, Imanpoor MR, Jafari V, Bennetau-Pelissero C. 2013. Reproductive impairment and endocrine disruption in goldfish by feeding diets containing SBM. Animal Reproduction Science, 139(1-4), 136-144. doi: 10.1016/j.anireprosci.2013.02.003
3. Brown AC, Stevenson LM, Leonard HM, Nieves-Puigdoller K, Clotfelter ED. 2014. Phytoestrogens β-sitosterol and genistein have limited effects on reproductive endpoints in a female fish, Betta splendens. BioMed Research International 2014(681396). doi: 10.1155/2014/681396
4. Chakraborty SB, Molnár T, Ardó L, Jeney G, Hancz C. 2015. Oral administration of Basella alba leaf methanol extract and genistein enhances the growth and non-specific immune responses of Oreochromis niloticus. Turkish Journal of Fisheries and Aquatic Sciences, 15(1), 167-173. doi: 10.4194 /1303-2712-v15_1_18
5. DiMaggio MA, Kenter LW, Breton TS, Berlinsky DL. 2016. Effects of dietary genistein administration on growth, survival and sex determination in southern flounder, Paralichthys lethostigma. Aquaculture Research, 47(1), 82-90. doi: 10.1111/are.12470
6. Dong C, Qiuyan J. 2016. Effects of genistein on the growth and gene expressions of the growth axis in the Nile tilapia Oreochromis niloticus. Feed Industry 6(007).
7. El-Sayed AFM, Abdel-Aziz ESH, Abdel-Ghani HM. 2012. Effects of phytoestrogens on sex reversal of Nile tilapia (Oreochromis niloticus) larvae fed diets treated with 17α-Methyltestosterone. Aquaculture 360-361(2012), 58-63. doi: 10.1016/j.aquaculture.2012.07.010
8. Gu M, Bai N, Zhang Y, Krogdahl Å. 2016. SBM induces enteritis in turbot Scophthalmus maximus at high supplementation levels. Aquaculture 464(2016), 286-295. doi: 10.1016/j.aquaculture.2016.06.035

9. Hernández MD, Martínez FJ, Jover M, García García B. 2007. Effects of partial replacement of fish meal by SBM in sharpsnout seabream (Diplodus puntazzo) diet. Aquaculture 263 (2007), 159–167. doi: 10.1016/j.aquaculture. 2006.07.040
10. Ikhwanuddin M, Lan SS, Abdul Hamid N, Fatihah Zakaria SN, Azra MN, Siti Aisah A, Abol-Munafi AB. (2015). The embryonic development of orange mud crab, Scylla olivacea (Herbst, 1796) held in the captivity. Iranian Journal of Fisheries Sciences, 14(4), 885-895.
11. Jourdehi AY, Sudagar M, Bahmani M, Hosseini SA, Dehghani AA, Yazdani MA. 2014. Comparative study of dietary soy phytoestrogens genistein and equol effects on growth parameters and ovarian development in farmed female beluga sturgeon, Huso huso. Fish Physiology and Biochemistry, 40(1), 117-128. doi: 10.1007/s10695-013-9829-z
12. Kaushik SJ, Cravedi JP, Lalles JP, Sumpter J, Fauconneau B, Laroche M. 1995. Partial or total replacement of fishmeal by SBM protein on growth, protein utilization, potential estrogenic or antigenic effects, cholesterolemia and flesh quality in rainbow trout, Oncorhynchus mykiss, Aquaculture, 133, 257–274. doi: 10.1016/0044-8486(94)00403-B
13. Ko K, Malison J, Reed J. 1999. Effect of genistein on the growth and reproductive function of male and female yellow perch Perca flavescens. Journal of World Aquaculture Society, 30(1999), 73–79. doi: 10.1111/j.1749-7345.1999.tb00319.x
14. Koshio S, Teshima SI, Kanazawa A, Watase T. 1993. The effect of dietary protein content on growth, digestion efficiency and nitrogen excretion of juvenile kuruma prawns, Penaeus japonicus. Aquaculture, 113(1-2), 101-114. doi: 10.1016/0044-8486(93)90344-X
15. Krogdahl Å, Bakke-McKellep AM, Baeverfjord G. 2003. Effects of graded levels of standard SBM on intestinal structure, mucosal enzyme activities, and pancreatic response in Atlantic salmon (Salmo salar L.). Aquacul. Nutr. 9, 361–371. doi: 10.1046/j.1365-2095.2003.00264.x
16. Luna, LG. (1968) Manual of histologic staining methods of the Armed Forces Institute of Pathology. 3rd Edition, McGraw-Hill, New York
17. Nezafatian E, Zadmajid V, Cleveland BM. 2017. Short‐term Effects of Genistein on the Reproductive Characteristics of Male Gibel Carp, Carassius auratus gibelio. Journal of the World Aquaculture Society, 48(5), 810-820. doi: 10.1111/jwas.12399
18. Pelissero C, Le Menn F, Kaushik S. 1991. Estrogenic effect of dietary SBM on vitellogenesis in cultured Siberian sturgeon Acipenser baeri, Gen. Comp. Endocrinology, 83: 447–457. doi: 10.1016/0016-6480(91)90151-U
19. Urán PA, Schrama JW, Rombout JHWM, Taverne-Thiele JJ, Obach A, Koppe W, Verreth JAJ. 2009. Time-related changes of the intestinal morphology of Atlantic salmon, Salmo salar L., at two different SBM inclusion levels. J. Fish Dis. 32, 733–744. doi: 10.1111/j.1365-2761.2009.01049.x
20. Zhang C, Rahimnejad S, Wang YR, Lu K, Song K, Wang L, Mai K. 2018. Substituting fish meal with soybean meal in diets for Japanese seabass (Lateolabrax japonicus): Effects on growth, digestive enzymes activity, gut histology, and expression of gut inflammatory and transporter genes. Aquaculture, 483(2018), 173-182. doi: 10.1016/j.aquaculture.2017.10.029