Gamma Cyhalothrin’in Dreissena polymorpha’daki Toksik Etkisinin Bazı Biyobelirteçlerle Belirlenmesi

Öz

Bu çalışmada, pestisitlerin hedef olmayan organizmalar olan tatlı su midyesi Dreissena polymorpha üzerindeki etkilerinin biyokimyasal tepkilerle belirlenmesi amaçlandı. Bu amaçla öncelikle Gamma Cyhalothrin (GCH) pestisitinin D. polymorpha üzerindeki akut toksisite değeri (LC50) standart statik yöntemle belirlenmiş ve probit analizi ile 13,64 mg/L hesaplanmıştır. Daha sonra 24 ve 96 saat boyunca ölümcül olmayan GCH konsantrasyonlarına maruz kalan D. polymorpha türünün süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx), katalaz (CAT) aktiviteleri, malondialdehit (MDA) ve glutatyon (GSH) düzeyi tepkileri belirlendi. Biyokimyasal biyobelirteçlerin yanı sıra histopatolojik yanıtlar da değerlendirildi. Sonuç olarak, bu çalışma GCH pestisitlerinin oksidatif stresi tetikleme yeteneklerini ortaya koydu. Ayrıca MDA, GSH düzeyleri, SOD, CAT, GPx aktiviteleri ve histopatolojik yanıtlar da D. polymorpha'da etkili bir biyobelirteç olarak kullanılabilir.

Anahtar Kelimeler

• Dreissena polymorpha
• gamma cyhalothrin
• biyobelirteç
• oksidatif stres
• histoloji

Determination of Toxic Effect of Gamma Cyhalothrin in Dreissena polymorpha by Some Biomarkers

Abstract

In this study, the effects of pesticides on the freshwater mussel Dreissena polymorpha, a non-target organism, were aimed to be determined through biochemical responses. For this purpose, first, the acute toxicity value (LC50) of Gamma Cyhalothrin (GCH) pesticide on D. polymorpha was determined by standard static method and the LC50 value was calculated as 13.64 mg/L using probit analysis. Then, superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activities, malondialdehyde (MDA) and glutathione (GSH) level responses of D. polymorpha organism exposed to GCH sublethal concentrations for 24 and 96 hours were determined. In addition to biochemical biomarkers, histopathological responses were evaluated. In conclusion, this study demonstrated the abilities of GCH pesticides to induce oxidative stress. Moreover, MDA, GSH levels, SOD, CAT, GPx activities and histopathological responses could be used as an effective biomarker in D. polymorpha.

Keywords

• Dreissena polymorpha
• gamma cyhalothrin
• biomarker
• oxidative stress
• histology

Kaynakça

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