Histopathological Effects of Formaldehyde (CH2O) on Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792)

Abstract

Formaldehyde is commonly used as a disinfectant and also in a control of fish disease in aquaculture sector. However, this widespread use, can lead to environmental degradation and can cause negative effects on the treated fish with. In this study 250 mg/L (1 hour) and 500 mg/L (45 min) concentrations of formaldehyde were used. From the results of the histopathological findings degeneration was determined in the epithelial cells and pilar in the gill lamellae, lymphoid infiltration interlamellar necrosis and degeneration of the muscle tissue, dilatation in the liver, congestion in veins, degeneration in hepatocytes, damage in the blood vessels of fish which were treated with formaldehyde. In conclusion; formaldehyde was found to have a negative impact in histological examination in applied rainbow trout. Therefore, it was concluded that it should be used consciously and according the needs in aquaculture.

Keywords

• Rainbow trout; Oncorhynchus mykiss; formaldehyde; histopathology

Gökkuşağı Alabalıklarında (Oncorhynchus mykiss Walbaum, 1792) Formaldehit (CH2O)’in Histopatolojik Etkileri

Öz

Formaldehit su ürünleri sektöründe balık hastalıklarının kontrolü ve dezenfektan olarak yaygın olarak kullanılmaktadır. Ancak, bu durum çeşitli çevresel olumsuzluklara sebep olabildiği gibi, uygulama yapılan balıklarda da olumsuz etkilere neden olabilmektedir. Bu çalışmada 250 mg/L (1 saat) ve 500 mg/L (45 dk) konsantrasyonlarında formaldehit uygulanmıştır. Çalışma sonucunda elde edilen histopatolojik bulgularda formaldehit uygulanan balıkların solungaç lamellalarda pilar ve epitel hücrelerinde dejenerasyon, interlamella alanlarda lenfoid ve mononükleer hücre infiltrasyonu ve nekroz, kas dokularında dejenerasyon, karaciğerlerinde genleşme, venalarında konjesyon, damarlarda hasar ve hepatositlerde dejenerasyon belirlenmiştir. Sonuç olarak formaldehit uygulamasının gökkuşağı alabalıklarında histolojik olarak olumsuz etkilere neden olduğu görülmüştür. Bu nedenle su ürünleri işletmelerinde bilinçli ve ihtiyaç dahilinde kullanılması gerektiği kanaatine varılmıştır.

Anahtar Kelimeler

• Gökkuşağı alabalığı; Oncorhynchus mykiss; formaldehit; histopatoloji

Kaynakça

1. Arda M, Seçer S, Sarrıeyyüpoğlu M. 2002. Balık hastalıkları. Ankara: Medisan Yayınları 142 p. [in Turkish]
2. Aoki T. 1992. Chemotherapy and drug resistance in fish farms in Japan. In: Sheriff M, Subasinghe RP, Aurthur JR, editors. Diseases in Asian aquaculture, fish health section. Philippines: Asian Fisheries Society. p. 519- 529.
3. Aydın S, Başaran N. 2012. Formaldehite maruziyetin toksikolojik açıdan değerlendirilmesi, the assessment of toxicological perspective of formaldehyde exposure: review. Turkiye Klinikleri J Pharm Sci. 1(2):95-110.
4. Barton BA, Iwama GK. 1991. Physiological changes in fish from stress in aquaculture with emphasis on the response and effeets of corticosteroids. Annu Rev Fish Dis. 1:3-26. doi: 10.1016/0959-8030(91)90019-G
5. Bayram H. 2008. The effect on some hematologic blood parameters of formaldehyde bath which was applied to Oncorhynchus mykiss [Master's Thesis]. Atatürk University. 44 p. [in Turkish]
6. Birici N, Şeker T, Balcı M, Çelik B, Kılıç A. 2014. Structural and economic analysis of trout breeding farms in Elazığ Province. Yunus Arş Bült. 2014(2):23-48. [in Turkish]
7. Björklund H, Bondestam J, Bylund G. 1991. Residues of oxytetracycline in wild fish and sediments from fish farms. Aquaculture. 86(4):359-367. doi: 10.1016/0044-8486(90)90324-g
8. Bulut C, Kubilay C, Akçimen U, Ceylan M. 2012. The effects on cortisol, glucose and lysozyme activity in different concentration of formaldehyde in rainbow trout. J FisheriesSciences.com. 6(4):321-330. doi: 10.3153/jfscom.akdeniz006 [in Turkish]

9. Chowdhury AR, Gautam AK, Patel KG, Trivedi HS. 1992. Steroidogenic inhibition in testicular tissue of formaldehyde exposed rats. Indian J Phys Pharm. 36(3):162-168. Cruz ER, Pitogo CL. 1989. Tolerance level and histopathological response of milkfish (Chanos chanos) fingerlings to formalin. Aquaculture. 78(2):135-145. doi: 10.1016/0044-8486(89)90027-6
10. Demir R. 2001. Histological painting techniques. Ankara: Palme Yayınları 320 p. [in Turkish]
11. Doyuk SA, Çolakoğlu FA. 2004. Kirleticilerin su ürünlerine etkileri. Ankara: Tarımsal Çevre ve Su Kirliliği. [in Turkish]
12. Dursun S. 1997. Hastalıklar bilgisi ders kitabı. İstanbul: Fatih Ofset 458 p. [in Turkish]
13. Grondel JL, Gloudemans AGM, Van Muiswinkel WB. 1985. The influence of antibiotics on the immune system, immuno-pharmokinetic. Investigations on the primary anti-SRBC response in carp (Cyprinus carpio L.) after oxytetracycline injection. J Fish Dis. 10(1):35-43. doi: 10.1111/j.1365-2761.1987.tb00716.x
14. Katalay S, Tuğlu İ, Minareci E. 2007. İzmir körfezinde yaşayan kaya balıklarında (Gobius niger) solungaç dokusunda oluşabilecek toksik etkinin ışık mikroskobik incelenmesi. Ege J Med. 46(2):61–66. [in Turkish]
15. Lom J, Dykova I. 1992. Protozoan parasites of fishes. Holland: Elsevier Science Publishers 315 p.
16. Luna LG. 1968. Manual of histologic staining methods of the armed forces institute of pathology. New York: McGraw-Hill Book Company 258 p.
17. Lundén T, Lilius E, Bylund G. 2002. Respiratory burst activity of rainbow trout (Oncorhynchus mykiss) phagocytes is modulated by antimicrobial drugs. Aquaculture. 207(3-4):203-212. doi: 10.1016/S0044-8486(01)00760-8
18. Lundén T, Bylund G. 2002. Effect of sulphadiazine and trimetoprim on the immune response of rainbow trout (Oncorhynchus mykiss). Vet Immunol Immunop. 85(1-2):99-108. doi: 10.1016/S0165-2427(01)00422-6
19. McMillan DB. 2007. Fish histology female reproductive systems. Netherlands: Springer Netherlands 598 p. doi: 10.1007/978-1-4020-5715-1
20. Meinelt BT, Pietrock M, Burnison K, Steinberg C. 2004. Formaldehyde toxicity is altered by calcium and organic matter. Blackwell Verlag. 21(2):121-124. doi: 10.1111/j.1439-0426.2004.00617.x
21. Miyaka CK, Alves de Souza JA, Torres RP. 1998. Effects of the administration of fish oil by gavage on activities of antioxidant enzymes of rat lymphoid organs. Gen Pharmacol. 30(5):759-762. doi: 10.1016/S0306-3623(97)00334-0
22. Nordøy A. 1991. Is there a rational use for n-3 fatty acids (fish oils) in clinical medicine? Drugs. 42(3):331-342. doi: 10.2165/00003495-199142030-00001
23. Öğüt H. 2005. Balıklarda stres. Karataş M, editör. Balık biyolojisi araştırma yöntemleri. Ankara: Nobel Yayın Dağıtım. p. 377-394. [in Turkish]
24. Pickering AD. 1993. Growth and stress in fish production. Aquaculture. 111(1-4):51-63. doi: 10.1016/0044-8486(93)90024-S
25. Sağlam N, Ural M. 2003. Macroscopic and microscopic ınvestigations on rainbow trout (Oncorhynchus mykiss) exposed to diffirent concentrations of copper sulphate (CuSO4). Fırat Uni Sci Eng. 15(1):89-97. [in Turkish]
26. Sarsılmaz M, Özen OA, Akpolat N, Kuş İ, Songur A. 1999. The histopathologic effects of inhaled formaldehyde on leydig cells of the rats in subacute period. Fırat Uni J Health Sci. 13(1):37-40.
27. Timur G, Timur M. 2003. Balık hastalıkları. İstanbul: Dilek Ofset 538 p. [in Turkish]
28. Uslu O, Türkman A. 1987. Su kirliliği ve kontrolü. Ankara: Başbakanlık Çevre Genel Müdürlüğü Yayınları. [in Turkish]
29. Üçüncü Sİ, Ergen G, Önen Ö, Kolbaşı Ö, Kolbaşı Tekkan B, Üreten M, Boz E, Seferoğlu K, Gökçe B. 2010. The effects of dioctyl adipate (DOA) on the liver histology of Labidochromis caeruleus Fryer, 1956 (Cichlidae, Teleostei). Kafkas Univ Vet Fak Derg. 16 (Suppl-B):197-203. doi: 10.9775/kvfd.2009.1132 [in Turkish]
30. Wedemeyer G. 1971. The stress of formalin treatments in rainbow trout (Oncorhynchus mykiss) and coho
31. salmon (Oncorhynchus kisutch). J Fish Res Board Can. 28(12):1899-1904. doi: 10.1139/f71-285
32. Wedemeyer G, Yasutake WT. 1974. Stress of formalin treatment in juveniles spring chinook salmon (Oncorhynchus tshawytsha) and steelhead trout (Salmo gairdneri). J Fish Res Board Can. 31(2):179-184. doi: 10.1139/f74-029
33. Wedemeyer GA, Barton BA, McLeay DJ. 1990. Stress and acclimation. In: Schreck C, Moyle P, editors. Methods for fish biology. Maryland: American Fisheries Society. p. 451-489.
34. Williams HA, Wootten R. 1981. Some effect of therapeutic levels of formalin and copper sulphate on blood parameters in rainbow trout. Aquaculture. 24:341-353. doi: 10.1016/0044-8486(81)90068-5
35. Yıldız HY. 2006. Plasma lysozyme levels and secondary stress response in rainbow trout (Oncorhynchus mykiss) after exposure to Leteux-Meyer Mixture. Turk J Vet Anim Sci. 30(2):265-269.