Chlorella vulgaris Beyerinck [Beijerinck] (Chlorophyta) Suşlarının Kesikli Kültür Sisteminde Yığın Kültürlerinin Üretimi Üzerine Bir Çalışma

Öz

Bu çalışmada, tatlısu birikintilerinde yaygın olarak bulunan, tek hücreli bir yeşil alg olan Chlorella vulgaris türünün izolasyonu, kültür koşullarının oluşturulması ve yığın kültürlerinin elde edilmesi amaçlanmıştır. C. vulgaris gıda, kozmetik ve eczacılık sektöründe kullanıldığı gibi, son yıllarda biyodizel üretiminde ve atık suların arıtımında da umut verici hale gelmiştir. Tatlısu birikintilerinden izole edilen C. vulgaris suşları kesikli kültür sistemi oluşturularak, 100, 200, 500, 1000, 2000 ve 4000 ml’lik hacimlerde üretimleri yapılmıştır. Suşların başlangıç inokulum miktarı, uygun pH, sıcaklık, havalandırma gibi etmenler denenerek optimum kültür koşulları oluşturulmuştur. 4000 ml’lik yığın kültürlerin 120. saatte yapılan hücre sayımlarında en yüksek değer AUFFH suşunda 6,8x10⁴ h/ml olarak tespit edilmiş bu suşu sırasıyla; TOH 6,7x10⁴ h/ml, UIK 6,3x10⁴ h/ml, GURH 6,2x10⁴ h/ml ve GUMSH 6,1x10⁴ h/ml suşları izlemiştir. Yaş ağırlıkları açısından 4000 ml’lik yığın kültürler için yine AUFFH 152 (g/2000 ml) ile en yüksek miktar elde edilirken onu sırasıyla TOH 129,6 (g/2000 ml), GURH 120,8 (g/2000 ml), GUMSH 84,3 (g/2000 ml), UIK 48,8 (g/2000 ml) takip etmiştir.

Anahtar Kelimeler

• Chlorella vulgaris,mikroalg,yığın kültür,kesikli kültür sistemi

A Study on the Production of Batch Cultures in Semi-Continuous Culture System of Chlorella vulgaris Beyerinck [Beijerinck] (Chlorophyta) Strains

Abstract

In this study, it is aimed to isolate Chlorella vulgaris strain, a single-celled green algae that is common in freshwater deposits, to establish culture conditions and to obtain batch cultures. C. vulgaris has been used in food, cosmetics and pharmaceutical industries as well as become encouraging for the production of biodiesel and the treatment of wastewater in recent years. C. vulgaris strains isolated from freshwater deposits were cultivated in a semi-continuous culture system of 100, 200, 500, 1000, 2000 and 4000 ml. Subsequently growth factors such as initial inoculum amount of the strains, appropriate pH, temperature and ventilation were tested in order to optimize the conditions. The highest value in the cell counts of 4000 ml batch cultures at the 120^th hours was determined as 6.8x10⁴ h/ml in AUFFH strain respectively the others were as follows: TOH 6.7x10⁴ h/ml, UIK 6.3x10⁴ h/ml, GURH 6.2x10⁴ h/ml and GUMSH 6.1x10⁴ h/ml. The highest amount in terms wet weight for 4000 ml batch cultures was obtained as AUFFH 152 (g/2000 ml), this value was followed by TOH 129.6 (g/2000 ml), GURH 120.8 (g/2000 ml), GUMSH 84.3 (g/2000 ml), UIK 48.8 (g/2000 ml).

Keywords

• Chlorella vulgaris,microalgae,batch culture,semi-continuous system

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