Effects of Different Protein Sources on Proteolotic Activity in Juveniles of Sterlet, Acipenser ruthenus Linnaeus 1758

Abstract

In this trial, Sterlet sturgeon, Acipenser ruthenus, larvae were fed by a standart feeding protocol from hatcing until 29^th day, and the proteolytic activity and the inhibition effect of different portein sources on protease activity were also measured. The data obtained from study showed that using soybean protein consantrate (38.57±10.44%) and corn gluten (43.07±1.72%) as protein source in mixed feeds of dulcite juvenile strains compared to other feed raw materials, was found to have a more positive effect on proteolytic enzyme activity in terms of statistics (P<0.05). When the binary combinations of feedstuffs were examined,  he lowest inhibition rate was obtained using 1: 1 (47.93 ± 1.72%) fish meal: soy protein concentrate, and this value was similar to that of  soybean protein consantrate and corn gluten as a single protien sources in the diets (P>0.05). The highest inhibitory effect was obtained by using 3: 1 ratio of wheat and brass bran  (82.39±7.23%).

Keywords

• protease activity
• protein sources
• Acipenser ruthenus
• sterlet
• inhibition effect

Farklı Protein Kaynaklarının Çuka Mersini, Acipenser ruthenus Linnaeus 1758, Juvenillerinde Proteaz Aktivitesi Üzerine Etkileri

Öz

Bu çalışmada, çuka balığı, Acipenser ruthenus,  larvaları yumurtadan çıkış anından itibaren 29 gün boyunca standart besleme protokolü ile beslenmiştir. Karma yeme geçiş yapılan 29. gündeki proteolitik aktivite ve farklı protein kaynaklarının proteaz aktivite üzerine inhibisyon etkileri ölçülmüştür. Çalışmadan elde edilen bilgiler ışığında, çuka balığı juvenillerinin karma yemlerinde protein kaynağı olarak soya protein konsantresi (%38,57±10,44) ve mısır gluten (%43,07±1,72) kullanmanın, diğer yem hammaddeleri ile karşılaştırıldığında, proteolitik enzim aktivitesini istatistiki açıdan daha olumlu etki yaptığı görülmüştür (P<0,05). Yem hammaddelerinin ikili kombinasyonları incelendiğinde ise, en düşük inhibisyon oranı balık unu: soya protein konsantresinin 1:1 oranda kullanılması (%47,93±1,72) ile elde edilmiş ve bu değerin soya protein konsantresi ve mısır gluteninin tek başlarına kullanılması ile elde edilen etki ile aynı seviyede olduğu tespit edilmiştir (P>0,05). En yüksek inhibe edici etki ise, buğday ile pirinç kepeğinin 3:1 oranda kullanılması ile oluşmuştur (%82,39±7,23).

Anahtar Kelimeler

• proteaz aktivitesi
• protein kaynakları
• Acipenser ruthenus
• çuka balığı
• inhibisyon etkisi

Kaynakça

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