Farklı Besi Ortamlarının Pseudopediastrum boryanum (Turpin) E. Hegewald İzolatlarının Biyokütle Üretimine Etkileri Üzerine Araştırma

Öz

Mikroalg, Pseudopediastrum boryanum (Turpin) E. Hegewald, atık su arıtımı ve biyodizel üretim potansiyelinden dolayı araştırma konusu olarak seçilmiştir. Bu araştırmada, iki besi ortamı (Allen ve BG-11) kullanılarak, kesikli kültür sisteminde P. boryanum'un büyüme ve biyokütle üretimi araştırılmıştır. Önceki çalışmalarımızda, P. boryanum dilüsyon tekniği kullanılarak, farklı tatlısu birikintilerinden izole edilmiştir. İzole edilen P. boryanum suşu, 270 mL besi ortamı + 30 mL süspansiyon kültür ile aşılanmış ve 16:8 aydınlık/karanlık fotoperiyodu uygulanmıştır. Optik yoğunluk 670 nm'de UV-Visible spektrofotometre kullanılarak tespit edilmiş ve suşların hücre sayımı damla sayım metodu kullanılarak yapılmıştır. Kültürlerin kuru ağırlık ve klorofil-a tayinleri de gerçekleştirilmiştir. Allen ortamında en yüksek hücre yoğunluğu (3,67x10⁶ hücre/mL), kuru ağırlık (0,032 g/mL) ve klorofil-a (16,39 μg L^-1) olarak tespit edilmiştir. P. boryanum’un büyüme oranları Allen besi ortamında 0,6676 d^-1 ve BG-11 ortamında 0,6021 d^-1 olarak bulunmuştur.

Anahtar Kelimeler

• Pseudopediastrum boryanum,optik yoğunluk,hücre yoğunluğu,üreme parametreleri,kültür koşulları

Investigating the Effect of Different Growth Media on Biomass Production of Pseudopediastrum boryanum (Turpin) E. Hegewald Isolates

Abstract

Microalgae Pseudopediastrum boryanum (Turpin) E. Hegewald was chosen as a subject for the present research due to its potential uses of wastewater treatment and biodiesel production. In the present study, we investigated the growth and biomass production of P. boryanum through use of semi-continuous cultures employing two growth media (Allen and BG-11). In our previous study, P. boryanum was isolated from different freshwater reservoir through the dilution technique. The isolated P. boryanum strain was inoculated with 270 mL of medium + 30 mL of suspension culture and the 16:8 light/dark photoperiod was applied. Optical density was recorded by using UV-Visible spectrophotometer at 670 nm, and cell count examination was performed through drop count method. Besides, dry weight and chlorophyll-a concentration of strain were determined. The highest cell density (3.67x10⁶ cells/mL), dry weight (0.032 g/mL) and chlorophyll-a (16.39 µgL^-1) production were observed in the Allen medium. Growth rates of P. boryanum were found to be 0.6676 d^-1 in the Allen and 0.6021 d^-1 in the BG-11 medium.

Keywords

• Pseudopediastrum boryanum,cell density, optical density,growth parameters,culture conditions

Kaynakça

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