Scenedesmus obliquus Suşlarının Farklı Besin Ortamlarındaki Büyüme Kinetiği

Öz

Bu çalışmada, farklı besi ortamları ve doğal maden sularının Scenedesmus obliquus (Turpin) Kützing’in büyüme dinamikleri
üzerine etkileri araştırılmıştır. S. obliquus suşları, Ankara (Türkiye) ilindeki çeşitli tatlı su kuyuları ve havuzlarından alınan
örneklerden izole edilmiştir. İzole edilen üç S. obliquus suşunun üretiminde, dört kültür besi ortamı ile dört doğal maden suyu
kullanılmıştır. Kültürlerin hücre yoğunluğu, kuru ağırlık miktarı, spesifik büyüme oranları, ikilenme süreleri ve klorofil-a miktarları
hesaplanmıştır. Sonuçlar, farklı kültür besi ortamları ve doğal mineralli suların, son hücre yoğunluğunu, biyokütleyi ve suşların
büyüme oranlarını önemli ölçüde etkilediğini göstermektedir. Bu üç suş içerisinde, ScnGP suşunda diğerlerine oranla hücre
yoğunluğu, biyokütle ağırlığı ve spesifik büyüme hızı açısından anlamlı bir fark bulunmuştur. Hücre yoğunluğu (7,4x104±1,3x103
hücre/mL), biyokütle miktarı (0,212±0,032 g/mL), spesifik büyüme oranı (0,024 h-1) ve klorofil-a (1,71±0,22 μg/mL) miktarı Bold
Wynne besi ortamında yetişen ScnGP suşunda diğer uygulamalara göre önemli ölçüde (P<0,05) daha yüksek tespit edilmiştir. Doğal
mineralli sulardan elde edilen sonuçların kültür besi ortamlarından elde edilen sonuçlar kadar iyi olduğu gözlenmiştir. Doğal
mineralli sulara çeşitli kimyasal bileşiklerin ve üreme faktörlerinin eklenerek üretimin artırılabileceği düşünülmektedir.

Anahtar Kelimeler

• Scenedesmus obliquus,mikroalg,hücre yoğunluğu,kuru ağırlık,doğal mineralli su

Growth Kinetics of Scenedesmus obliquus Strains in Different Nutrient Media

Öz

In this study, the effects of different media and natural mineral waters on the
growth dynamics of Scenedesmus obliquus (Turpin) Kützing were investigated.
S. obliquus strains were isolated from various freshwater wells and pools in
Ankara, Turkey. In the production of three S. obliquus strains, both four culture
medium and four natural mineral waters were used. Cell density, dry weight,
specific growth rates, duplication time and chlorophyll-a of the cultures were
calculated. The results showed that the different culture media and natural mineral
waters significantly affected the final cell density, biomass and growth rates of
strains. In three isolates, there was a significant difference in ScnGP strain in
terms of cell density, biomass weight and specific growth rate compared to others.
Cell density (7.4x104±1.3x103cells/mL), biomass amount (0.212±0.032g/mL),
specific growth rate (0.024 h-1) and chlorophyll-a (1.71±0.22μg/mL) of ScnGP
grown in Bold Wynne medium were significantly (P<0.05) higher than that of
grown in all other treatments. It has been determined that the results obtained from
natural mineral waters were as good as the results obtained from culture media. It
can be thought that various chemical compounds and reproductive factors can be
added to increase the production within the natural mineral waters.

Anahtar Kelimeler

• Scenedesmus obliquus,microalgae,cell density,dry weight,natural mineral water

Kaynakça

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