Sazan (Cyrpinus carpio), Sudak (Sander lucioperca), Gümüşi Havuz Balığı (Carassius gibelio) ve Kerevit (Astacus leptodactylus) Örneklerinde Serum Lizozim Aktivitelerinin Karşılaştırılması

Öz

Bu çalışmada, ilkbahar ve sonbahar dönemlerinde üç farklı bölgeden elde edilen sazan (Cyprinus carpio), sudak (Sander lucioperca), gümüşi havuz balığı (Carassius gibelio) ve kerevit (Astacus leptodactylus) örneklerinde diffüzyon agar metodu kullanılarak serum lizozim aktiviteleri incelenmiştir. Lizozim aktivitesi en yüksek seviyede sudak balıklarında, ardından sırayla sazan, gümüşi havuz balığı ve kerevit örneklerinde bulunmuştur. Sazan, gümüşi havuz balığı ve kerevit arasında anlamlı bir fark bulunmazken sudak balıkları ve diğer türler arasında anlamlı bir farklılık olduğu belirlenmiştir (p<0,05). Sazan, gümüşi havuz balığı ve kerevitlerde ilkbahar ve sonbahar dönemleri arasında serum lizozim düzeylerinde fark anlamlı bulunurken (p<0,05), sudak balıklarında mevsimler arasındaki fark anlamlı bulunmamıştır. Bu sonuçlar, sucul ekosistemindeki farklı türlerin doğuştan gelen bağışıklık sistemindeki lizozim aktivitesinin farklı olduğunu göstermiştir.

Anahtar Kelimeler

• Doğal bağışıklık
• stres
• mevsimsel değişim
• difüzyon agar metodu
• su ürünleri

Comparative Studies on Serum Lysozyme Activities of Common Carp (Cyprinus carpio), Pikeperch (Sander lucioperca), Prussian Carp (Carassius gibelio) and Crayfish (Astacus leptodactylus)

Öz

In this study, serum lysozyme activity was studied by the agar well diffusion assay in various species of fish (common carp, Cyprinus carpio, pikeperch, Sander lucioperca, prussian carp, Carassius gibelio) and crayfish (Astacus leptodactylus) obtained from three different regions in spring and autumn in 2013. The highest levels of lysozyme activity were found in pikeperch fish, followed by common carp, prussian carp, and crayfish, respectively. There was no significant difference between common carp, prussian carp, and crayfish, but it was found a significant between pikeperch fish and the other species (p<0.05). There was a significant difference of serum lysozyme levels between spring and autumn in common carp, prussian carp and crayfish (p<0.05). There was no significant difference between seasons in pikeperch fish. These results showed different of the lysozyme activity in the fish innate immune system in the aquatic ecosystem of different species.

Anahtar Kelimeler

• innate immunite
• stress
• seasonal variation
• agar well diffusion assay
• fisheries

Kaynakça

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