Bakteriyel Balık Patojenleri için Kromojenik Besiyerlerinin Değerlendirilmesi

Öz

Akuakültür işletmelerinde etkin hastalık kontrolü ve ekonomik kayıpların önlenmesi için bakteriyel balık hastalıklarının doğru ve hızlı tanımlanması büyük önem taşımaktadır. Genel olarak kullanılan agar besiyerlerinde çok çeşitli bakteri türünün üremesi gözlenirken, yeni kromojenik besiyerleri, biyokimyasal ve serolojik test gerektirmeden renkli koloni görünümüyle tahmini ön tanımlama imkânı sunmaktadır. Bu çalışmada yakın zamanda ticarileşen kromojenik besiyerlerinde (chromID® CPS® Elite, chromID® CPS® Elite / Columbia CNA +5% sheep blood agar, chromID® Vibrio, chromID® MRSA Smart ve chromID® Staphylococcus aureus Elite (BioMérieux, France)) bakteriyel balık patojenlerinin üremesi ve koloni renklenmeleri tespit edilmiştir. ChromID® CPS® Elite ve chromID® CPS® Elite / Columbia CNA +5% sheep blood agarın test edilen 5 farklı bakteriyel balık patojeni (Aeromonas hydrophila, Lactococcus garvieae, Pseudomonas fluorescens, Yersinia ruckeri ve Vibrio anguillarum) için en uygun besiyeri olduğu, ancak sadece L. garvieae’nin yeşil koloni görünümü ile ürediği belirlenmiştir. Vibrio parahaemolyticus ve Vibrio cholerae’nın hızlı teşhisi için geliştirilen chromID® Vibrio besiyerinde V. anguillarum kolonileri koyu pembe/mor renkli olarak gözlenmiş, chromID® MRSA Smart ve chromID® S. aureus Elite besiyerlerinin bakteriyel balık patojenlerinin üremesi için uygun olmadığı belirlenmiştir.

Anahtar Kelimeler

• Kromojenik besiyeri
• balık hastalıkları
• balık patojenleri
• izolasyon

Assessment of Chromogenic Media in Bacterial Fish Pathogens

Abstract

Accurate and quick identification of bacterial fish disease has great importance for effective disease control and prevent economic loss in aquaculture facilities. Generally used agar media allow the growth of wide-range bacteria but new chromogenic media results in colored colonies that lead to presumptive identification without requiring biochemical or serological tests. In this study, the recently commercialized chromogenic media; chromID® CPS® Elite, chromID® CPS® Elite / Columbia CNA +5% sheep blood agar, chromID® Vibrio, chromID® MRSA Smart and chromID® Staphylococcus aureus Elite (BioMérieux, France) were tested with growth performance and colony coloration of bacterial fish pathogens. chromID® CPS® Elite and chromID® CPS® Elite / Columbia CNA +5% sheep blood agar were detected the most suitable culture media to grow for all five isolated fish pathogens (i.e., Aeromonas hydrophila, Lactococcus garvieae, Pseudomonas fluorescens, Yersinia ruckeri and Vibrio anguillarum). Of which, L. garvieae was the only one observed with green colonies. chromID® Vibrio designed for V. parahaemolyticus and V cholerae was lead dark pink/purple colonies by V. anguillarum. The chromID® MRSA Smart and chromID® S. aureus Elite were detected not effective for incubation of bacterial fish pathogens.

Keywords

• Chromogenic media
• fish disease
• fish pathogens
• isolation

Kaynakça

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