Investigation of the Aromatase Cytochrome P450 gene (CYP19) P1.1-PvuII Polymorphism in Native Turkish Cattle Breeds

Rümeysa Yiğit; Yasemin Öner

doi:10.46897/livestockstudies.2015437

Investigation of the Aromatase Cytochrome P450 gene (CYP19) P1.1-PvuII Polymorphism in Native Turkish Cattle Breeds

Abstract

The Aromatase Cytochrome P450 gene (CYP19) encodes the key enzyme in estrogen biosynthesis and has been proposed as a candidate marker for fertility and milk yield traits in cattle. In this study, the CYP19 P1.1-PvuII polymorphism was investigated in five native Turkish cattle breeds. DNA samples obtained from a previous project were used to amplify a 405 bp fragment of the CYP19 gene promoter region, followed by PCR-RFLP analysis. Allele and genotype frequencies were estimated using POPGENE32, and Hardy-Weinberg equilibrium was assessed using a chi-square test. Two alleles (A and B) and two genotypes (AA and AB) were identified among 172 individuals; the BB genotype was not observed. The A allele was monomorphic (1.000) in the Turkish Grey breed and ranged from 0.886 to 0.960 in the remaining breeds. All breeds were in Hardy-Weinberg equilibrium (p>0.05), reflecting a low level of diversity at this locus.

Keywords

Supporting Institution

TUBITAK

Project Number

Project No: 1919B012406861

Ethical Statement

The genomic DNA samples used in this study were obtained from a previously completed project. The present study was carried out with the approval of the relevant institutional Animal Experiments Ethics Committee (Approval Date: 12.11.2024, Decision No: 2024-15/01).

Thanks

Project No: 1919B012406861

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Details

Primary Language

English

Subjects

Zootechny (Other), Animal Science, Genetics and Biostatistics

Journal Section

Research Article

Authors

Rümeysa Yiğit This is me
Türkiye

Yasemin Öner ^*
0000-0002-2904-8986
Türkiye

Early Pub Date

October 12, 2026

Publication Date

-

Submission Date

August 10, 2026

Acceptance Date

September 8, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.46897/livestockstudies.2015437

IZ

https://izlik.org/JA68JZ77XZ

Cite

RIS / Bibtex

APA

Yiğit, R., & Öner, Y. (2026). Investigation of the Aromatase Cytochrome P450 gene (CYP19) P1.1-PvuII Polymorphism in Native Turkish Cattle Breeds. Livestock Studies, Advanced Online Publication. https://doi.org/10.46897/livestockstudies.2015437

AMA

1.Yiğit R, Öner Y. Investigation of the Aromatase Cytochrome P450 gene (CYP19) P1.1-PvuII Polymorphism in Native Turkish Cattle Breeds. Livestock Studies. 2026;(Advanced Online Publication). doi:10.46897/livestockstudies.2015437

Chicago

Yiğit, Rümeysa, and Yasemin Öner. 2026. “Investigation of the Aromatase Cytochrome P450 Gene (CYP19) P1.1-PvuII Polymorphism in Native Turkish Cattle Breeds”. Livestock Studies, no. Advanced Online Publication. https://doi.org/10.46897/livestockstudies.2015437.

EndNote

Yiğit R, Öner Y (October 1, 2026) Investigation of the Aromatase Cytochrome P450 gene (CYP19) P1.1-PvuII Polymorphism in Native Turkish Cattle Breeds. Livestock Studies Advanced Online Publication

IEEE

[1]R. Yiğit and Y. Öner, “Investigation of the Aromatase Cytochrome P450 gene (CYP19) P1.1-PvuII Polymorphism in Native Turkish Cattle Breeds”, Livestock Studies, no. Advanced Online Publication, Oct. 2026, doi: 10.46897/livestockstudies.2015437.

ISNAD

Yiğit, Rümeysa - Öner, Yasemin. “Investigation of the Aromatase Cytochrome P450 Gene (CYP19) P1.1-PvuII Polymorphism in Native Turkish Cattle Breeds”. Livestock Studies. Advanced Online Publication (October 1, 2026). https://doi.org/10.46897/livestockstudies.2015437.

JAMA

1.Yiğit R, Öner Y. Investigation of the Aromatase Cytochrome P450 gene (CYP19) P1.1-PvuII Polymorphism in Native Turkish Cattle Breeds. Livestock Studies. 2026. doi:10.46897/livestockstudies.2015437.

MLA

Yiğit, Rümeysa, and Yasemin Öner. “Investigation of the Aromatase Cytochrome P450 Gene (CYP19) P1.1-PvuII Polymorphism in Native Turkish Cattle Breeds”. Livestock Studies, no. Advanced Online Publication, Oct. 2026, doi:10.46897/livestockstudies.2015437.

Vancouver

1.Rümeysa Yiğit, Yasemin Öner. Investigation of the Aromatase Cytochrome P450 gene (CYP19) P1.1-PvuII Polymorphism in Native Turkish Cattle Breeds. Livestock Studies. 2026 Oct. 1;(Advanced Online Publication). doi:10.46897/livestockstudies.2015437