Orpin ve Menke Besi Ortamlarında Neocallimastix sp'nin Selülotik Enzim Aktivitelerinin Karşılaştırılması

Öz

Neocallimastix sp., benzersiz lignoselülotik enzimlere sahip Anaerobik Gut Fungusları (AGF) ailesinin bir üyesidir ve genellikle in vitro olarak iki temel besi ortamında da yetiştirilir. Enzimatik aktivitelerin tespiti için Orpin besi ortamı tercih edilirken, fermantasyon sırasında oluşan gazların değerlendirilmesi için daha çok Menke besi ortamı tercih edilmektedir. Bu iki ortamın hedeflenen amaçlar için etkili olduğu gösterilmiş olmasına rağmen, bu besi ortamlarındaki çeşitli selülotik enzimlerin aktivitelerini karşılaştırmak için hiçbir girişimde bulunulmamıştır. Bu çalışmada Neocallimastix sp.'nin bu iki besiyerinde de 7 güne kadar büyüme hızı ölçülmüş ve her ikisinde de benzer bir gelişim gözlenmiştir (p>0.05). Ayrıca Menke besi ortamının Aviselaz’ın 5. (p<0.001) ve 7. (p<0.0.1) günleri ve 5. güne kadar [0 (p<0.001), 3 (p<0.01), 5 (p<0.0001)] Ksilanaz aktivitesi için daha iyi olduğu belirlenmiştir. Orpin besi ortamı ise tüm zaman noktalarında [0 (p<0.001), 3 (p<0.0001), 5 (p<0.05, 7 (p<0.001)] üstün KMSaz aktivitesi sergilemiştir. Selülaz için, aktiviteler her iki ortamda 0. ve 3. günlerde hemen hemen aynı ölçülürken, Menke'de (p<0.0001) 5. günde ve Orpin'de 7. günde (p<0.01) daha yüksek olduğu görülmüştür. Sonuç olarak Ksilanaz enzim çalışmalarında menke besi ortamınız daha iyi sonuç verdiği tespit edilmiştir.

Anahtar Kelimeler

• Anaerobik fungus
• Enzim aktivitesi
• Orpin
• Menke

Comparing Cellulotic Enzyme Activities of Neocallimastix sp. in Orpin’s and Menke’s Media

Öz

Neocallimastix sp. is a member of the Anaerobic Gut Fungi (AGF) family with unique lignocellulotic enzymes, and it is usually grown in two basic media in vitro. Orpin’s mediais preferred for the detection of enzymatic activites while Menke’s is mostly chosen for evaluating the gases produced during the fermantation. Although these two media were shown to be effective for their targeted purposes, no attempt has been made to compare the activities of various cellulotic enzymes in them. In this study, we measured the growth rate of Neocallimastix sp. in these two media up to 7 days and A similar progress was observed in both (p>0.05). We also discovered that Menke’s media was better for the 5th (p<0.001) and 7th (p<0.0.1) days of Avicellase and for up to 5 days [0 (p<0.001), 3 (p<0.01), 5 (p<0.0001)] of Xylanase activity. Orpin’s media, on the other hand, displayed superior CMCase activity in all time points[0 (p<0.001), 3 (p<0.0001), 5 (p<0.05, 7 (p<0.001)]. As for Cellulase, the activities were measured virtually the same for the both media on the 0th and the 3rd days whereas they were higher in Menke’s (p<0.0001) on the 5th, and in Orpin’s on the 7th day (p<0.01). As a result, in Xylanase enzyme studies, it has been determined that menke media gives better results.

Anahtar Kelimeler

• Anaerobic fungi
• Enzyme activity
• Orpin
• Menke

Teşekkür

We would like to thank Mehmet Sait Ekinci and Emin Özköse for their intellectual and financial contributions. Halit YÜCEL 100/2000 Graduate Scholarship gratefully thanks to YÖK.

Kaynakça

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