Molecular İdentification and Phylogeny of Some Hypocreales Members Isolated from Agricultural Soils

Öz

Hypocreanlean fungi are include important plant pathogens around the world. Head blight and crown rot disease of cereals caused by these species are responsible for large economic losses due to reduction in seed quality and contamination of grain with their mycotoxins. Although morphologic and biochemical tests are still fundamental there is an increasing more towards molecular diagnostics of these fungi. This paper reviews to PCR identification of Hypocreanlean fungi isolated from agricultural soil from Eskisehir. Five Hypocreanlean fungi belong to 4 different genera as Bionectria, Fusarium, Gibberella and Nectria were isolated from 56 soil samples. DNA of these strains were isolated by glass beads and vortexing extraction method and used for PCR amplification with universal fungal specific primers. The internal transcribed spacer (ITS) regions of fungal ribosomal DNA (rDNA) were sequenced by CEQ 8000 Genetic Analysis System. The ITS-5.8S sequences obtained in this study were compared with those deposited in the GenBank Database. Phylogenetic position of investigated closely related Hypocreanlean fungi was determined.

Anahtar Kelimeler

• Hypocreanlean,PCR,ITS,Phylogeny,Eskişehir

Tarımsal Topraklardan İzole Edilen Bazı Hypocreales Üyelerinin Moleküler Teşhisi ve Filogenisi

Öz

Hypocreanlean mantarları Dünya’daki önemli bitki patojenleridir. Bu türlerin sebep olduğu baş tahribat ve tahıl hasar hastalığı, tohum kalitesinde azalma ve tahılın mikotoksinler ile bulaşması nedeniyle büyük ekonomik kayıplardan sorumludur. Morfolojik ve biyokimyasal testler hala temel olsa da, bu mantarların moleküler teşhisine yönelim giderek artmaktadır. Bu makale, Eskişehir’deki tarım topraklarından izole edilmiş olan Hypocreanlean mantarlarının PCR ile identifikasyonunu incelemektedir. Bionectria, Fusarium, Gibberella ve Nectria olmak üzere 4 farklı cinse ait 5 Hypocreanlean mantarı 56 farklı toprak numunesinden izole edilmiştir. Bu suşların DNA'sı, cam boncuklar ve vorteks ekstraksiyon yöntemi ile izole edilmiş ve üniversal fungal spesifik primerler ile PCR amplifikasyonu için kullanılmıştır. Fungal ribozomal DNA'nın (rDNA) iç transkripsiyonlu ayırıcı (ITS) bölgeleri, CEQ 8000 Genetik Analiz Sistemi ile dizilenmiştir. Bu çalışmada elde edilen ITS-5.8S dizileri, GenBank veri tabanında depolanan dizilerle karşılaştırılmıştır. İncelenen birbirine yakın akraba Hypocreanlean mantarlarının filogenetik konumu belirlenmiştir.

Anahtar Kelimeler

• Hypocreanlean,PCR,ITS,Filogeni,Eskişehir

Kaynakça

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