Fomes fomentarius‘dan elde edilen çeşitli formülasyonların hastane izolatlarına karşı antimikrobiyal etkisi

Öz

Bu çalışmanın amacı, F. fomentarius formülasyonlarının antimikrobiyal aktivitesini araştırmaktır. Etanol ve sudan hazırlanan F. fomentarius özütlerinin ve çeşitli formülasyonların (emülsiyon, merhem, pat, krem ve jel) antimikrobiyal etkinlik seviyeleri belirlendi. Bu amaçla ekstreleri, Klebsiella pneumoniae, Acinetobacter baumannii, Staphylococcus aureus, vankomisine dirençli enterokok, (VRE +), Escherichia coli, Candida krusei; C. albicans, C. tropicalis, C. guilliermondii ve C. glabrata içeren hastane izolatlarına karşı antimikrobiyal aktivite açısından test etmek için disk difüzyon yöntemi kullanılmıştır. Karşılaştırma için bazı standart antibiyotikler kullanılmıştır. Merhem formülasyonu (No. 4), antibiyotiklere kıyasla bakteriler üzerinde iyi bir antimikrobiyal etki göstermiştir (9.6 - 16.1 mm.). Ekstrelerin Candida türlerine karşı antibiyotiklerden daha etkili olduğu bulunmuştur. 4 Nolu formülasyonun, setil alkolünün ve sodyum lauril sülfat (SLS) içeriğinin koruyucu doğası nedeniyle daha etkili olduğu düşünülmüştür. F. fomentarius etanol ekstresi, F. fomentarius su ekstresi ve jel formülasyonu benzer aktivite göstermiştir; bununla birlikte, diğer formülasyonlar (emülsiyon, pat, krem), preparasyonlarında bulunan polimerlerin, yağın ve alkollerin stabilize edici etkileri nedeniyle genellikle daha etkili olmuştur. Bu çalışmanın bulguları, klinik olarak etkili yeni antibiyotik ilaçları keşfetme olasılığını ortaya koymaktadır ve geleneksel ilaçlar ile modern ilaçlar arasındaki ilişkiyi anlamada faydalı olabilir.

Anahtar Kelimeler

• Fomes fomentarius,β-glukan,antimikrobiyal aktivite,sodyum lauril eter sülfat,setil alkol

The antimicrobial effect of various formulations obtained from Fomes fomentarius against hospital isolates

Öz

The purpose of this study was to investigate the antimicrobial activity of formulations of F. fomentarius. The antimicrobial efficacy levels of extracts of F. fomentarius prepared from ethanol, water and of various formulations (emulsion, ointment, paste, cream and gel) were determined. For this purpose, the disk diffusion method was used to test the extracts for antimicrobial activity against hospital isolates including Klebsiella pneumoniae, Acinetobacter baumannii, Staphylococcus aureus, vancomycin-resistant enterococci (VRE+), Escherichia coli, Candida krusei, C. albicans, C. tropicalis, C. guilliermondii and C. glabrata. Some standard antibiotics were used for comparison. The ointment formulation (No. 4) showed a good antimicrobial effect on bacteria compared to the antibiotics (9.6 - 16.1 mm.). The extracts were found to be more effective than the antibiotics against Candida species. Formulation No. 4 was considered to be more effective because of the protective nature of its cetyl alcohol and sodium lauryl sulfate (SLS) content. F. fomentarius ethanol extract, F. fomentarius water extract and gel formulation showed similar activity; however, other formulations (emulsion, paste, cream) were generally more effective due to the stabilizing effects of the polymers, oil and alcohols present in their preparations. The findings of this study establish the possibility of discovering new clinically effective antibiotic drugs and could be useful in understanding the relationship between traditional remedies and modern medicines.

Anahtar Kelimeler

• Fomes fomentarius,β-glukan,antimicrobial activity,sodium lauryl ether sulphate,cethyl alchol

Kaynakça

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