Tuz Gölü (Türkiye) Mikrofungus Çeşitliliği ve Dağılımı

Öz

Bu çalışmanın amacı, geleneksel ve moleküler tanılama verileri kullanılarak Tuz Gölü'ndeki mikrofungus çeşitliliğinin aydınlatılması ve popülasyon dinamikleriyle ilişkilendirilmesidir. Mikrofunguslar, mevsimsel olarak üç istasyondan alınan toplam 24 örnekten, iki tip ortam kullanılarak membran filtrasyon yöntemi ile izole edilmiştir. DRBC besiyerinin kullanıldığında koloni sayıları tuzla havuz suyunda 68 CFU / 100 ml ve göl suyunda 86 CFU / ml olmuştur; DRBC17 besiyerinde ise her iki tip su örneğinde de koloni sayısı aynı 21 CFU / 100 ml bulunmuştur. DRBC ortamında 19 cinse ait 52 farklı tür izole edilirken, DRBC17 üzerinde 11 cinse ait 30 farklı tür tespit edilmiştir. Toplamda 21 cinse ait 54 farklı tür izole edilmiştir. Tür çeşitliliğine göre önde gelen cinsler Penicillium (15 tür), Aspergillus (8 tür), Alternaria (8 tür), Cladosporium (5 tür), Arthrinium (2 tür) 'dur. Bu çalışma, Tuz Gölü mikobiyotasının yüksek bir çeşitliliğe sahip olduğunu ve izole edilen türlerin büyük bir kısmının tuzlu ortamlara adapte olmuş türler olduğunu göstermiştir.

Anahtar Kelimeler

• Mantar çeşitliliği
• Hipersalin
• Tuzlu su
• Bolluk
• Frekans

Diversity and Distribution of Microfungi in Tuz Lake, Turkey

Öz

The aim of this study is to help illuminate the microfungus diversity in Tuz Lake using traditional and molecular diagnostic data and to correlate it with population dynamics. Microfungi were isolated from a total of 24 samples taken from three stations seasonally by membrane filtration method using two types of media. When using DRBC medium, colony counts were 68 CFU / 100 ml in saltpan water and 86 CFU / ml in lake water. On the DRBC17 medium, the number of colonies in both types of water samples was found to be the same 21 CFU / 100 ml. While 52 different species belonging to 19 genera have been isolated on DRBC medium, 30 different species belonging to 11 genera were detected on DRBC17. In total, 54 different species belonging to 21 genera were isolated. According to the variety of species, the leading genera are Penicillium (15 spp), Aspergillus (8 spp), Alternaria (8 spp), Cladosporium (5 spp), Arthrinium (2 spp). This study showed that Tuz Lake mycobiota has a high diversity and most of the isolated species are adapted to saline environments.

Anahtar Kelimeler

• Fungal diversity
• Hypersaline
• Saltern
• Abundance
• Frequency

Kaynakça

1. Ali, I., Kanhayuwa, L., Rachdawong, S., & Rakshit, S. K. (2013). Identification, Phylogenetic Analysis and Characterization of Obligate Halophilic Fungi Isolated from a Man-Made Solar Saltern in Phetchaburi Province, Thailand. Annals Microbiol., 63(3), 887-895.
2. Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic Local Alignment Search Tool., J. Molecular Biol., 215(3), 403-410.
3. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W., & Lipman, D. J. (1997). Gapped BLAST and PSI-BLAST: a New Generation of Protein Database Search Programs. Nucleic Acids Res., 25(17), 3389-3402.
4. Andrews, S., & Pitt, J. (1987). Further Studies on The Water Relations of Xerophilic Fungi, Including Some Halophiles. Microbiol., 133(2), 233.
5. Asan, A. (2004). Aspergillus, Penicillium and Related Species Reported from Turkey. Mycotaxon, 89(1), 155-157.
6. Barnett, H., & Hunter, B. (1999). Illustrated Genera of Imperfect Fungi (4th ed.): St. Paul: APS.
7. Birbir, M., Calli, B., Mertoglu, B., Bardavid, R. E., Oren, A., Ogmen, M. N., & Ogan, A. (2007). Extremely Halophilic Archaea from Tuz Lake, Turkey, and the Adjacent Kaldirim and Kayacik Salterns. World J. Microbiol. Biotechnol., 23(3), 309-316.
8. Buchalo, A., Nevo, E., Wasser, S., Oren, A., & Molitoris, H. (1998). Fungal Life in The Extremely Hypersaline Water of the Dead Sea: First Records. Proc. Royal Soc. B, 265(1404), 1461-1465.

9. Butinar, L., Sonjak, S., Zalar, P., Plemenitaš, A., & Gunde-Cimerman, N. (2005). Melanized Halophilic Fungi are Eukaryotic Members of Microbial Communities in Hypersaline Waters of Solar Salterns. Botanica Marina, 48(1), 73-79.
10. Butinar, L., Zalar, P., Frisvad, J. C., & Gunde-Cimerman, N. (2005). The Genus Eurotium–Members of Indigenous Fungal Community in Hypersaline Waters Of Salterns. FEMS Microbiol. Ecol., 51(2), 155-166.
11. Cantrell, S. A., & Baez-Félix, C. (2010). Fungal Molecular Diversity of a Puerto Rican Subtropical Hypersaline Microbial Mat. Fungal Ecol., 3(4), 402-405.
12. Cantrell, S. A., Casillas-Martinez, L., & Molina, M. (2006). Characterization of Fungi from Hypersaline Environments of Solar Salterns using Morphological and Molecular Techniques. Mycol. Res., 110(8), 962-970.
13. Cantrell, S. A., Gunde-Cimerman, N., Zalar, P., Acevedo, M., & Báez-Félix, C. (2013). Fungal Communities of Young and Mature Hypersaline Microbial Mats. Mycologia, 827–836.
14. Cronin, E. A., & Post, F. J. (1977). Report of a Dematiaceous Hyphomycete from the Great Salt Lake, Utah. Mycologia, 69(4), 846-847.
15. DasSarma, S., & DasSarma, P. (2012). Halophiles. In Encyclopedia of Life Sciences. Chichester: Sons Ltd.
16. Dengiz, O., Ozcan, H., Koksal, E. S., Baskan, O., & Kosker, Y. (2010). Sustainable Natural Resource Management and Environmental Assessment in the Salt Lake (Tuz Golu) Specially Protected Area. Environ. Monitor. Assess., 161(1-4), 327-342.
17. Diguță, C. F., Proca, I. G., Jurcoane, Ș., & Matei, F. (2018). Molecular Characterization by PCR-RFLP of Indigenous Fungal Isolates from Hypersaline Stream Water in România. Folia Microbiol., 1-8.
18. Ellis, M. (1965). Dematiaceous Hyphomycetes.: Commonwealth Mycological Institute, Kew.
19. Geer, L. Y., Marchler-Bauer, A., Geer, R. C., Han, L., He, J., He, S., . . . Bryant, S. H. (2010). The NCBI BioSystems database. Nucleic Acids Res. 38(suppl_1), D492-D496.
20. Gonsalves, V., Nayak, S., & Nazareth, S. (2012). Halophilic Fungi in a Polyhaline Estuarine Habitat. J. Yeast Fungal Res., 3(3), 30-36. Graham, G., Mayers, P., & Henry, R. (1994). A simplified method for the preparation of fungal genomic DNA for PCR and RAPD analysis. Biotechniques, 16(1), 48-50.
21. Gunde-Cimerman, N., Butinar, L., Sonjak, S., Turk, M., Uršič, V., Zalar, P., & Plemenitaš, A. (2005). Halotolerant and Halophilic Fungi from Coastal Environments in the Arctics. In N. Gunde-Cimerman, A. Oren, & A. Plemenitaš (Eds.), Adaptation to Life at High Salt Concentrations in Archaea, Bacteria, and Eukarya: Springer.
22. Gunde-Cimerman, N., Ramos, J., & Plemenitas, A. (2009). Halotolerant and Halophilic Fungi. Mycol. Res., 113(11), 1231-1241.
23. Gunde-Cimerman, N., & Zalar, P. (2014). Extremely Halotolerant and Halophilic Fungi Inhabit Brine in Solar Salterns Around the Globe. Food Technol. Biotechnol. 52(2), 170-179.
24. Gunde-Cimerman, N., Zalar, P., de Hoog, S., & Plemenitaš, A. (2000). Hypersaline Waters in Salterns–Natural Ecological Niches for Halophilic Black Yeasts. FEMS Microbiol. Ecol., 32(3), 235-240.
25. Hocking, A. D., Miscamble, B. F., & Pitt, J. (1994). Water Relations of Alternaria alternata, Cladosporium cladosporioides, Cladosporium sphaerospermum, Curvularia lunata and Curvularia pallescens. Mycol. Res., 98(1), 91-94.
26. King Jr, A., Hocking, A., & Pitt, J. (1979). Dichloran-Rose Bengal Medium for Enumeration and Isolation of Molds from Foods. Appl.Environ. Microbiol., 37(5), 959-964.
27. Kis-Papo, T., Grishkan, I., Oren, A., Wasser, S., & Nevo, E. (2001). Spatiotemporal Diversity of Filamentous Fungi in the Hypersaline Dead Sea. Mycol. Res., 105(06), 749-756.
28. Kis-Papo, T., Oren, A., Wasser, S., & Nevo, E. (2003). Survival of Filamentous Fungi in Hypersaline Dead Sea Water. Microb. Ecol., 45(2), 183-190.
29. Klich, M. (2002). Identification of Common Aspergillus Species. 122 pp. Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands.
30. Magurran, A. E. (2013). Measuring biological diversity: John Wiley & Sons.
31. Maria, G., & Sridhar, K. (2003). Diversity of Filamentous Fungi on Woody Litter of Five Mangrove Plant Species from The Southwest Coast of India. Fungal Divers., 14, 109-126.
32. Mc Carthy. (1996). Chromas, (Version 1.45). School of Health Science, Griffifth University, Gold Coast Campus: Queensland, Australia, .
33. Murray, M., & Thompson, W. (1980). Rapid Isolation of High Molecular Weight Plant DNA. Nucleic Acids Res., 8(19), 4321-4326.
34. Mutlu, M. B., Martiez-Garcia, M., Santos, F., Pena, A., Guven, K., & Anton, J. (2008). Prokaryotic Diversity in Tuz Lake, a Hypersaline Environment in Inland Turkey. FEMS microbiol. Ecol., 65(3), 474-483.
35. Nayak, S. S., Gonsalves, V., & Nazareth, S. W. (2012). Isolation and Salt Tolerance of Halophilic Fungi from Mangroves and Solar Salterns in Goa-India. Indian J. Geo-Marine Sci., 41, 164-172.
36. Nazareth, S., & Gonsalves, V. (2014). Aspergillus penicillioides—a True Halophile Existing in Hypersaline and Polyhaline Econiches. Ann. Microbiol., 64(1), 397-402.
37. Nazareth, S., Gonsalves, V., & Nayak, S. (2012). A First Record of Obligate Halophilic Aspergilli from the Dead Sea. Indian J Microbiol., 52(1), 22-27.
38. Nazareth, S. W., & Gonsalves, V. (2014). Halophilic Aspergillus penicillioides from Athalassohaline, Thalassohaline, and Polyhaline Environments. Front Microbiol., 5, 412.
39. Oren, A. (2016). Life in High-Salinity Environments. In Manual of Environmental Microbiology, Fourth Edition: American Society of Microbiology.
40. Özgök, Ö., & İlhan, S. (2020). Diversity and Distribution of Dematiaceous Fungi in Çamaltı Saltern in İzmir Province, Turkey. J. Fungus, 11(1), 29-39.
41. Pitt, J. (1979). The genus Penicillium and its Teleomorphic States Eupenicillium and Talaromyces. 634 pp. London etc.
42. Pitt, J., & Hocking, A. (2009). Fungi and Food Spoilage: Springer Verlag.
43. Samson, R. A., Hoekstra, E. S., & Frisvad, J. C. (2004). Introduction to Food-and Airborne Fungi: Centraalbureau voor Schimmelcultures (CBS).
44. Samson, R. A., Seifert, K. A., Kuijpers, A. F., Houbraken, J., & Frisvad, J. C. (2004). Phylogenetic Analysis of Penicillium Subgenus Penicillium using Partial β-tubulin Sequences. Stud. Mycol., 49, 175-200.
45. Sarma, V., & Hyde, K. D. (2001). A Review on Frequently Occurring Fungi in Mangroves. Fungal Diver., 8, 1-34.
46. Sklenář, F., Jurjević, Ž., Zalar, P., Frisvad, J. C., Visagie, C. M., Kolařík, M., . . . Hubka, V. (2017). Phylogeny of Xerophilic Aspergilli (Subgenus Aspergillus) and Taxonomic Revision of Section Restricti. Stud. Mycol., 88, 161-236.
47. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., & Kumar, S. (2011). MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol. Biol. Evol., 28(10), 2731-2739.
48. Tsang, C.-C., Tang, J. Y. M., Lau, S. K. P., & Woo, P. C. Y. (2018). Taxonomy and Evolution of Aspergillus, Penicillium and Talaromyces in the Omics Era – Past, Present and Future. Comput. Struct. Biotechnol. J., 16, 197-210.
49. UNESCO. (https://whc.unesco.org/en/tentativelists/5824/). Lake Tuz Special Environmental Protection Area (SEPA).
50. Uygun, A., & Şen, E. (1978). The Salt Lake Basin and Natural Resources I: Geochemistry of the Brine of the Salt Lake (Central Anatolia-Turkey). Bulletin of the Geological Society of Turkey, 21, 113-120.
51. White, T., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. In PCR protocols: a guide to methods and applications (pp. 315-322): Academic Press.
52. Woudenberg, J., Groenewald, J., Binder, M., & Crous, P. (2013). Alternaria Redefined. Stud Mycol., 75, 171-212.
53. Zalar, P., De Hoog, G., Schroers, H.-J., Crous, P., Groenewald, J. Z., & Gunde-Cimerman, N. (2007). Phylogeny and Ecology of the Ubiquitous Saprobe Cladosporium sphaerospermum, with Descriptions of Seven New Species from Hypersaline Environments. Stud Mycol., 58(1), 157-183.