Zeytin Değirmeni Atıklarından Yeni İzole Edilen Bir Maya Candida tropicalis; Lipaz Üretimi İçin Besiyeri Kompozisyonun Optimizasyonu

Öz

Bu çalışmada bir zeytin değirmeni atık örneğinden yeni izole edilen bir mikroorganizma lipaz üretimi için taranmıştır. Bu yeni suş 18S rDNA analizi ile tanımlanmış ve suşun %92 oranında Candida tropicalis (yuvarlakmaya) olduğu tespit edilmiştir. Lipaz üretim ortamına azot ve karbonhidrat kaynakları ilave edilerek lipaz üretiminin optimizasyonu gerçekleştirilmiş, ayrıca lipaz üretimini artırmak için pH ve sıcaklık parametrelerinin etkisi incelenmiştir. Suş için maksimum büyüme koşulları 4.0 pH ortamında ve 30°C büyüme sıcaklığında tespit edilmiştir. Çeşitli azot kaynaklarının lipaz üretimi üzerindeki etkisi araştırıldığında, amonyum sülfatın lipaz üretimini arttırdığını, üre, pepton ve kazeinin ise belirgin bir etki göstermediğini saptanmıştır. Birtakım yağların artırmasına rağmen lipaz üretim ortamında çeşitli şekerlerin bulunması lipaz üretimini verimli bir şekilde artırmamıştır. En yüksek lipaz aktivitesi, üretim ortamına %1 amonyum sülfat ve %1 zeytinyağı ilavesiyle 10.67 U/ml olarak belirlenmiştir.

Anahtar Kelimeler

• Candida tropicalis
• Lipaz üretimi
• Optimizasyon

A Novel Yeast Isolated From Olive Mill Waste Candida tropicalis; Optimization of Medium Composition For Lipase Production

Öz

A novel microorganism that was isolated from an olive mill waste sample was screened for lipase production. This novel strain was identified and determined by 18S rDNA analysis and it was detected that the strain was 92% Candida tropicalis in ratio. Optimization of lipase production was carried out by the addition of nitrogen and carbohydrate sources into the lipase production medium as well as the effect of pH and temperature parameters were studied to increase the lipase production. Maximum growth conditions for the strain were detected at 4.0 pH medium and 30°C growth temperature. The effect of various nitrogen sources on lipase production showed that ammonium sulfate increased lipase production whereas urea, peptone and casein did not show a distinct effect. In addition presence of various sugars in the lipase production medium did not increase the lipase production efficiently although some oils did. The highest lipase activity was determined as 10.67 U/ml, with the addition of 1% ammonium sulfate and 1% olive oil into the production medium.

Anahtar Kelimeler

• Candida tropicalis
• Lipase Production
• Optimization

Kaynakça

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