Yıl 2018, Cilt 9 , Sayı 1, Sayfalar 28 - 35 2018-04-25

Kültür Mantarı (Agaricus bisporus) Genomuna Özel Yüksek Miktarda Basit Dizi Tekrarı (SSR) Markörünün Geliştirilmesi ve Yüksek Çözünürlükte Fiziksel Genom Haritasının Oluşturulması
High-Throughput Genomic Simple Sequence Repeat (SSR) Marker Development and Construction of a High Resolution Physical Map in Button Mushroom (Agaricus bisporus) Genome

Ali Tevfik Uncu [1] , Ayse Ozgur Uncu [2]


Kültür mantarı [Agaricus bisporus (J.E. Lange) Imbach], bir model organizma olmasının yanı sıra dünya genelinde yetiştirilen bir yenilebilir mantar türüdür. Bilimsel ve ekonomik önemine rağmen A. bisporus genomuna özel moleküler markör geliştirme çalışmaları ancak yakın zamanda gerçekleştirilmeye başlanmış olup, oldukça kısıtlı sayıdadır. Bu çalışma kapsamında A. bisporus genomuna özel 3943 adet yeni basit dizi tekrarı (SSR) markörü geliştirilmiştir. Geliştirilen markörler A. bisporus kromozomlarına dengeli şekilde dağılmakta olup, genomun sekans-spesifik markörler ile doyurulmasını sağlamaktadır. Çalışma kapsamında tanıtılmakta olan yüksek sayıdaki A. bisporus’a özel yeni DNA markörü, bu türde gerçekleştirilecek moleküler genetik çalışmalar için kıymetli bir kaynak teşkil edecektir.

Button mushroom [Agaricus bisporus (J.E. Lange) Imbach], is both a model organism and a worldwide cultivated edible mushroom species. Despite its scientific and economic relevance, efforts to develop molecular markers in A. bisporus genome are scarce and relatively recent. The present research reports the development of 3943 novel simple sequence repeat (SSR) markers specific to the A. bisporus genome. The marker set is well-distributed among the 13 A. bisporus chromosomes, saturating the entire genome with sequence-specific markers. The large number of novel, A. bisporus specific DNA markers introduced in the present work constitute a valuable resource for molecular genetic research in button mushroom, including strain identification and protection, and gene/QTL (Quantitative Trait Locus) mapping.

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Birincil Dil en
Konular Fen
Bölüm MİKOLOJİ
Yazarlar

Yazar: Ali Tevfik Uncu (Sorumlu Yazar)
Kurum: Necmettin Erbakan Üniversitesi, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü
Ülke: Turkey


Yazar: Ayse Ozgur Uncu
Kurum: Necmettin Erbakan Üniversitesi, Fen Fakültesi, Biyoteknoloji Bölümü
Ülke: Turkey


Tarihler

Yayımlanma Tarihi : 25 Nisan 2018

Bibtex @araştırma makalesi { mantar391682, journal = {Mantar Dergisi}, issn = {}, eissn = {2147-6845}, address = {}, publisher = {Selçuk Üniversitesi}, year = {2018}, volume = {9}, pages = {28 - 35}, doi = {10.30708/mantar.391682}, title = {High-Throughput Genomic Simple Sequence Repeat (SSR) Marker Development and Construction of a High Resolution Physical Map in Button Mushroom (Agaricus bisporus) Genome}, key = {cite}, author = {Uncu, Ali Tevfik and Uncu, Ayse Ozgur} }
APA Uncu, A , Uncu, A . (2018). High-Throughput Genomic Simple Sequence Repeat (SSR) Marker Development and Construction of a High Resolution Physical Map in Button Mushroom (Agaricus bisporus) Genome. Mantar Dergisi , 9 (1) , 28-35 . Retrieved from https://dergipark.org.tr/tr/pub/mantar/issue/36725/391682
MLA Uncu, A , Uncu, A . "High-Throughput Genomic Simple Sequence Repeat (SSR) Marker Development and Construction of a High Resolution Physical Map in Button Mushroom (Agaricus bisporus) Genome". Mantar Dergisi 9 (2018 ): 28-35 <https://dergipark.org.tr/tr/pub/mantar/issue/36725/391682>
Chicago Uncu, A , Uncu, A . "High-Throughput Genomic Simple Sequence Repeat (SSR) Marker Development and Construction of a High Resolution Physical Map in Button Mushroom (Agaricus bisporus) Genome". Mantar Dergisi 9 (2018 ): 28-35
RIS TY - JOUR T1 - High-Throughput Genomic Simple Sequence Repeat (SSR) Marker Development and Construction of a High Resolution Physical Map in Button Mushroom (Agaricus bisporus) Genome AU - Ali Tevfik Uncu , Ayse Ozgur Uncu Y1 - 2018 PY - 2018 N1 - DO - T2 - Mantar Dergisi JF - Journal JO - JOR SP - 28 EP - 35 VL - 9 IS - 1 SN - -2147-6845 M3 - UR - Y2 - 2018 ER -
EndNote %0 Mantar Dergisi High-Throughput Genomic Simple Sequence Repeat (SSR) Marker Development and Construction of a High Resolution Physical Map in Button Mushroom (Agaricus bisporus) Genome %A Ali Tevfik Uncu , Ayse Ozgur Uncu %T High-Throughput Genomic Simple Sequence Repeat (SSR) Marker Development and Construction of a High Resolution Physical Map in Button Mushroom (Agaricus bisporus) Genome %D 2018 %J Mantar Dergisi %P -2147-6845 %V 9 %N 1 %R %U
ISNAD Uncu, Ali Tevfik , Uncu, Ayse Ozgur . "High-Throughput Genomic Simple Sequence Repeat (SSR) Marker Development and Construction of a High Resolution Physical Map in Button Mushroom (Agaricus bisporus) Genome". Mantar Dergisi 9 / 1 (Nisan 2018): 28-35 .
AMA Uncu A , Uncu A . High-Throughput Genomic Simple Sequence Repeat (SSR) Marker Development and Construction of a High Resolution Physical Map in Button Mushroom (Agaricus bisporus) Genome. Değerlendirme süreci. 2018; 9(1): 28-35.
Vancouver Uncu A , Uncu A . High-Throughput Genomic Simple Sequence Repeat (SSR) Marker Development and Construction of a High Resolution Physical Map in Button Mushroom (Agaricus bisporus) Genome. Mantar Dergisi. 2018; 9(1): 35-28.