Türkiye Mikobiyotası İçin Yeni Kayıt Gyromitra’nın Morfolojik ve Filogenetik Olarak Ortaya Çıkarılması

Yıl 2018, Cilt: 9 Sayı: 2, 176 - 181, 25.10.2018

Ayten Dizkırıcı , İsmail Acar , Ayşenur Kalmer , Yusuf Uzun

Öz

Bu çalışmada, Discinaceae ailesine ait olan Gyromitra
brunnea Underw. Türkiye için ilk kez kaydedilmiştir. Hem morfolojik hem de
moleküler veriler kullanılarak iki örnek tanımlanmıştır. Rapor edilen yeni
kayıt türün kısa açıklaması, makro ve mikromorfolojiyle ilgili fotoğraflar ile
birlikte verilmiş ve kısaca tartışılmıştır. Ek olarak, Gyromitra brunnea'nın filogenetik konumu, 28S (LSU) rRNA gen
dizisine dayalı olarak oluşturulan filogenetik ağaçta gösterilmiştir.

Anahtar Kelimeler

Gyromitra brunnea, fungal taksonomi, yeni kayıt, LSU, filogeni

Morphology and Phylogeny Reveal a New Record Gyromitra for Turkish Mycobiota

Öz

In the current study, Gyromitra brunnea Underw. belonging to the family Discinaceae was recorded for the first
time from Turkey. Two candidate samples were described
by using both morphological and molecular data. Short description of the
newly reported species was given together with its photographs related to macro
and micromorphologies and discussed briefly. Additionally, phylogenetic
position of Gyromitra brunnea was
indicated in the phylogenetic tree constructed based on the sequence of 28S
(LSU) rRNA gene.

Anahtar Kelimeler

Gyromitra brunnea, fungal taxonomy, new record, LSU, phylogeny

Kaynakça

• Abbott, S.P., Currah S., The Helvellaceae: systematic revision and occurrence in northern and Northwestern North America. Mycotaxon 62:1–125 (1997).
• Asemaninejad, A., Weerasuriya, N., Gloor, G.B., Lindo, Z., Thorn, R.G., New Primers for Discovering Fungal Diversity Using Nuclear Large Ribosomal DNA. PLoS ONE 11(7): e0159043 (2016). doi:10.1371/journal.pone.0159043
• Doyle, J.J., Doyle, J.L., A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin. 19, 11-15 (1987).Fries, E.M., Systema mycologicum. Vol. 2. Geifswald. 620 p (1823).
• Hansen, K., Pfister, D.H., Systematics of the Pezizomycetes--the operculate discomycetes. Mycologia, 98 (6): 1029–40 (2006).
• Harmaja, H., A wider and more natural concept of the genus Gyromitra Fr. Karstenia 9:9–12 (1969).
• Index Fungorum, http://www.indexfungorum.org/names/Names.asp, last accession: 17 May 2017.
• Kirk, P.M., Cannon, P.F., Minter, D.W., Stalpers, J.A., Dictionary of the Fungi. 10th ed. Wallingford: CABI. p.512. UK (2008).
• Kuo, M. 2012. Gyromitra brunnea. Retrieved from the MushroomExpert.Com Web site: http://www.mushroomexpert.com/gyromitra_brunnea.html (accessed: 04.04.18) Liu, K., Liu, L., Porras-Alfaro, A., Kuske, C.R., Eichorst, S.A., Xie, G., Accurate, Rapid Taxonomic Classification of Fungal Large-Subunit rRNA Genes. Applied and Environmental Microbiology, 78 (5): 1523–1533 (2012).
• McKnignt, K.H., McKnignt, V.B., A Field Guide to Mushrooms: North America. Houghton Mifflin Harcourt, United States (1987).
• Methven, A.S. Zelski, S.E. Miller, A.N., A molecular phylogenetic assessment of the genus Gyromitra in North America. Mycologia, 105(5), 1306–1314 (2013).
• Mycobank database, Discina brunnea, http://www.mycobank.org/BioloMICS.aspx?Table=Mycobank&Rec=221484&Fields=All, (last accession: 10.03.2018).
• Mycobank database, Discina, http://www.mycobank.org/BioloMICS.aspx?TableKey=14682616000000067&Rec=57997&Fields=All, (last accession: 17.03.2018).
• Raja, H.A. Miller, A.N. Pearce, C.J. Oberlies, N.H., Fungal Identification Using Molecular Tools: A Primer for the Natural Products Research Community. J. Nat. Prod. 2017, 80, 756−770 (2017), DOI: 10.1021/acs.jnatprod.6b01085
• Sesli E., Denchev C.M. Onward (Continuously Updated). Mycotaxon Webpage. Available online at http://www. mycotaxon.com/resources/weblists.html (2014).Solak M.H., Işıloğlu M, Kalmış E., Allı H., Macrofungi of Turkey. Checklist. İzmir, Turkey: Üniversiteliler Ofset (2015).
• Stielow, J.B., Lévesque, C.A., Seifert, K.A., Meyer ,W., Irinyi, L., Smits, D., Renfurm, R., Verkley, G.J.M., Groenewald, M., Chaduli, D., One fungus, which genes? Development and assessment of universal primers for potential secondary fungal DNA barcodes. Persoonia 35, 242–263 (2015).
• Tamura, K., Stecher, G., Peterson, D., Filipski, A., M., Kumar, S., MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol. Biol. Evol., 30(12): 2725–2729 (2013).
• Undan, J.Q., Alfonso, D.O., Dulay, R.M., Leon, A.M., Kalaw, S.P. Undan J.R., Reyes, R.G., Molecular identification and phylogeny of different macrofungi in Mt. Bangkay, Cuyapo, Nueva Ecija, Philippines based on ITS nrDNA region. Advances in Environmental Biology, 10(12) December 2016, Pages: 35-42 (2016).
• Van Vooren, N., Moreau, P.A., Essai taxinomique sur le genre Gyromitra Fr. Sensu lato (Pezizales), 1. Introductionet systématique. Ascomycete.org 1(1): 3-6 (2009).