Comparison Of Some Effects Of Wild-Grown And Cultivated Forms Of Ganoderma Lucidum In Turkey On Chicken Embryos

Yıl 2018, Cilt: 9 Sayı: 2, 188 - 195, 25.10.2018

Haluk Özparlak , Bülent Çelik Döndü Balta

Öz

Ganoderma lucidum (Curtis) P. Karst is a mushroom which used for
alternative medicine worldwide particularly in China, also known as
“Immortality Mushroom or Reishi”. In recent years, cultivated G. lucidum is also produced in Turkey
and consumed by people especially as tea, coffee and tablets. The aim of this study was to determine the some
effects of wild-grown and cultivated G. lucidum in Turkey on the development of chicken embryos, as an alternative experimental
animal, for the first time. Two experiments were carried out for this purpose.
Wild-grown and cultivated forms of G.
lucidum were tested at the same doses in the first and second experiment,
respectively. Aqueous extracts of G. lucidum at different doses (1750 µg/egg, 875 µg/egg and 219
µg/egg) were injected into the fertilized chicken eggs at 8th day of incubation
as well as sterile bi-distilled water for control groups. Following
parameters were examined on 11th day of the incubation: rates of dead and
abnormal embryo, malformation types, live and relative embryo weights. In
addition, some of the embryos were totally stained with Alizarin Red-S method
for bone development. Both wild-grown and cultivated
forms of G. lucidum at doses tested did
not present significant embryotoxic and teratogenic effects as well as did not
affect the bone development of embryos at the macroscopic level.

Anahtar Kelimeler

Alizarin Red-S, Chick Embryo, embryotoxicity, Ganoderma, teratogenicity

Ganoderma lucidum’un Türkiye’deki Yabani Ve Kültür Formlarının Tavuk Embriyoları Üzerindeki Bazı Etkilerinin Karşılaştırılması

Öz

“Ölümsüzlük mantarı”
veya “Reishi” olarak da bilinen Ganoderma
lucidum (Curtis) P. Karst tüm dünyada ve özellikle de Çin’de alternatif tıp
alanında kullanılan bir mantardır. Son yıllarda G. lucidum’un kültür formu Türkiye’de de yetiştirilmektedir ve halk
tarafından özellikle çay, kahve ve tablet olarak tüketilmektedir. Bu çalışmayla
Türkiye’deki yabani ve kültür G. lucidum’un
alternatif bir deney hayvanı olarak tavuk embriyoları üzerindeki bazı
etkilerinin ilk kez tespit edilmesi amaçlandı. Bu amaçla iki deney
gerçekleştirildi. G. lucidum’un
birinci deneyde yabani ve ikinci deneyde kültür formu aynı dozlarda test
edildi. Deneylerde döllü tavuk yumurtalarına kuluçkanın 8. gününde G. lucidum’un sulu ekstraktları farklı
dozlarda (1750 µg/yumurta, 875 µg/yumurta ve 219 µg/yumurta) enjekte edildi.
Kontrol gruplarına steril bi-distile su enjekte edildi. Kuluçkanın 11. gününde
ölü ve anormal embriyo oranları, malformasyon tipleri, canlı ve rölatif embriyo
oranları tespit edildi. Ayrıca kemik gelişiminin belirlenebilmesi için
embriyoların bir kısmı total olarak Alizarin Red-S yöntemiyle boyandı. G. lucidum’un hem yabani hem de kültür
formları test edilen dozlarda önemli bir embriyotoksik ve teratojenik etki
göstermedi. Ayrıca embriyoların kemik gelişimini de makroskobik seviyede
etkilemedi.

Anahtar Kelimeler

Alizarin Red-S, civciv embriyosu, Ganoderma, teratojenite, embriyotoksisite, teratojenite

Kaynakça

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