Fomes fomentarius‘dan elde edilen çeşitli formülasyonların hastane izolatlarına karşı antimikrobiyal etkisi

Yıl 2019, Cilt: 10 Sayı: 2, 103 - 109, 30.10.2019

Gülşah Gedik Görkem Dülger Hülya Asan Anıl Özyurt Hakan Allı Ahmet Asan

Öz

Bu çalışmanın amacı, F. fomentarius formülasyonlarının
antimikrobiyal aktivitesini araştırmaktır. Etanol ve sudan hazırlanan F. fomentarius özütlerinin ve çeşitli
formülasyonların (emülsiyon, merhem, pat, krem ve jel) antimikrobiyal etkinlik
seviyeleri belirlendi. Bu amaçla ekstreleri, Klebsiella pneumoniae, Acinetobacter
baumannii, Staphylococcus aureus, vankomisine dirençli
enterokok, (VRE +), Escherichia coli,
Candida krusei; C. albicans, C. tropicalis, C. guilliermondii ve C. glabrata içeren hastane izolatlarına
karşı antimikrobiyal aktivite açısından test etmek için disk difüzyon yöntemi
kullanılmıştır. Karşılaştırma için bazı standart antibiyotikler kullanılmıştır.
Merhem formülasyonu (No. 4), antibiyotiklere kıyasla bakteriler üzerinde iyi
bir antimikrobiyal etki göstermiştir (9.6 - 16.1 mm.). Ekstrelerin Candida türlerine karşı
antibiyotiklerden daha etkili olduğu bulunmuştur. 4 Nolu formülasyonun, setil
alkolünün ve sodyum lauril sülfat (SLS) içeriğinin koruyucu doğası nedeniyle
daha etkili olduğu düşünülmüştür. F.
fomentarius etanol ekstresi, F.
fomentarius su ekstresi ve jel formülasyonu benzer aktivite göstermiştir;
bununla birlikte, diğer formülasyonlar (emülsiyon, pat, krem),
preparasyonlarında bulunan polimerlerin, yağın ve alkollerin stabilize edici
etkileri nedeniyle genellikle daha etkili olmuştur. Bu çalışmanın bulguları,
klinik olarak etkili yeni antibiyotik ilaçları keşfetme olasılığını ortaya
koymaktadır ve geleneksel ilaçlar ile modern ilaçlar arasındaki ilişkiyi
anlamada faydalı olabilir.

Anahtar Kelimeler

Fomes fomentarius, β-glukan, antimikrobiyal aktivite, sodyum lauril eter sülfat, setil alkol

The antimicrobial effect of various formulations obtained from Fomes fomentarius against hospital isolates

Öz

The purpose of this
study was to investigate the antimicrobial activity of formulations of F. fomentarius. The antimicrobial
efficacy levels of extracts of F.
fomentarius prepared from ethanol, water and of various formulations
(emulsion, ointment, paste, cream and gel) were determined. For this purpose,
the disk diffusion method was used to test the extracts for antimicrobial
activity against hospital isolates including Klebsiella pneumoniae, Acinetobacter baumannii, Staphylococcus aureus,
vancomycin-resistant enterococci (VRE+),
Escherichia coli, Candida krusei, C. albicans, C. tropicalis, C. guilliermondii
and C. glabrata. Some standard
antibiotics were used for comparison. The ointment formulation (No. 4) showed a
good antimicrobial effect on bacteria compared to the antibiotics (9.6 - 16.1 mm.).
The extracts were found to be more effective than the antibiotics against Candida species. Formulation No. 4 was
considered to be more effective because of the protective nature of its cetyl
alcohol and sodium lauryl sulfate (SLS) content. F. fomentarius ethanol extract, F. fomentarius water extract and gel formulation showed similar
activity; however, other formulations (emulsion, paste, cream) were generally
more effective due to the stabilizing effects of the polymers, oil and alcohols
present in their preparations. The findings of this study establish the
possibility of discovering new clinically effective antibiotic drugs and could
be useful in understanding the relationship between traditional remedies and
modern medicines.

Anahtar Kelimeler

Fomes fomentarius, β-glukan, antimicrobial activity, sodium lauryl ether sulphate, cethyl alchol

Kaynakça

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