Saleplerden (Orkide) İzole Edilen Endofitik Fusarium spp.’nin Moleküler Tanımlanması ve Filogenetik Çeşitlilik Analizleri

Yıl 2021, Cilt: 12 Sayı: 2, 163 - 171, 31.10.2021

Yüksel Gezgin

Öz

Türkiyenin endemik bitkisi Salep (orkide) kök ve yumrulardan izole edilmiş endofitik fungus türlerinin moleküler teknikler kullanılarak tanımlanması son derece önemlidir. Bu çalışmada Salep (Orchid) bitkisinden izole edilen endofitik Fusarium Link türlerinin tanımlanması amaçlanmıştır. Bu amaçla beta-tubulin (β-tubulin: benA), Internal Transcribed Spacer (ITS) ve Translation Elongation Factor (EF) gen bölgeleri Polimeraz Zincir Reaksiyonu (PZR) ile özel primerler kullanılarak çoğaltılmıştır. Daha sonra tüm PZR ürünlerinin dizi analizi yapılmış ve GenBank’daki diğer ilgili diziler ile karşılaştırılmıştır. Fusarium izolatları tür düzeyinde Fusarium redolens Wollenw. (n=7) ve Fusarium oxysporum Schlecht. (n=3) olarak tanımlanmışlardır. Filogenetik analizler the β-tubulin, ITS ve EF gen dizilerini temel alarak gerçekleştirilmiştir. Bu çalışmada, ilk olarak üç farklı gen bölgesinin çoğaltılması içeren moleküler tanımlama yöntemleri kullanılarak Türkiye’deki saleplerden (orkidelerden) izole edilen endofitik Fusarium spp. izolatları tür düzeyinde tanımlanmıştır.

Anahtar Kelimeler

Salep, Orkide, Endofitik fungus, Polimeraz Zincir Reaksiyonu, Fusarium redolens, Fusarium oxysporum

Destekleyen Kurum

Ege Üniversitesi

Teşekkür

Çalışmalarım esnasında laboratuvar imkânlarını esirgemeyen Sayın Arş. Gör. Dr. Arzu YILDIRIM’a çok teşekkür ederim.

Phylogenetic Diversity Analysis and Molecular Identification of Endofitic Fusarium spp. Isolated From Salep (Orchid)

Öz

The endophytic fungi species isolated from Salep (Orchid) roots and tubers, endemic plants of Turkey, their identification using the molecular techniques is utmost important. The aim of the present study was to identify the species of endophytic Fusarium Link isolated from Salep (Orchid). For this purpose, beta-tubulin (β-tubulin: benA), Internal Transcribed Spacer (ITS) and Translation Elongation Factor (EF) gene regions were amplified by Polymerase Chain Reaction using specific primers. Then, all PCR products were sequenced and compared with the other related sequences in GenBank. The Fusarium isolates were identified to species level as Fusarium redolens Wollenw. (n=7) and Fusarium oxysporum Schlecht. (n=3). The phylogenetic analysis was carried out based on the β-tubulin, ITS and EF gene sequences. In the present study, Fusarium spp. isolated from Saleps (Orchids) in Turkey were firstly identified by molecular identification methods including amplification of the three different gene region.

Anahtar Kelimeler

Salep, Orchid, Endophytic fungi, Polymerase Chain Reaction, Fusarium redolens, Fusarium oxysporum

Kaynakça

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• http://www.blast.ncbi.nlm.nih.gov (Son erişim tarihi: Haziran 2021)