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Bitki Doku Kültüründe İridoit Glikozitler

Yıl 2013, Cilt: 25 Sayı: 3, 115 - 133, 23.12.2013
https://doi.org/10.7240/201332363

Öz

Iridoit glycosides are monoterpene derivatives biosynthesized from isoprenoids (terpenes). They are synthesized naturally in many different dicotyl plants and they protect plants against biotic and abiotic attacks. Iridoit glycosides are powerful phytochemicals. They exhibit antimicrobial, antitumor, anti-cardiac, anti-inflammatory, anti-hepatoma and antioxidant and neuroprotective effects on human health.

 

Iridoid glycosides are found in many medicinal plants and are responsible for their pharmaceutical activities. Based on these facts, increasing irioid glycoside production by plant tissue culture methods has become one of the important research topics recently. After their production by plant tissue culture methods, they can be extracted and subjected to quantitative and qualitative analyses with different devices.

Kaynakça

  • Babaoğlu M, Gürel E ve Özcan S (2001) Bitki Biyoteknolojisi I. Doku Kültürü ve Uygulamaları. Selçuk Üniversitesi Basımevi, 374.
  • Tasdemir D, Scapozza L, Zerbe O, Linden A, Çalis I, Sticher O ( 1999) Iridoid Glycosides of Leonurus persicus. J. Nat. Prod., 62: 811- 816.
  • Crişan G, Vlase L, Balica G, Muntean D, Ştefanescu C, Paltinean R, Tamaş M, Leucuta S (2010) LC /MS Analysis of Aucubin and Catalpol of Some Veronica Species. Farmacia, 58: 237- 247.
  • Willinger G, Dobler D (2001) Selective sequestration of iridoid glycosides from their host plants in Longitarsus flea beetles. Biochemical Systematics and Ecology, 29: 335- 346.
  • Dinda B, Roy Chowdhury R, Mohanta B C (2009) Naturally Occurring Iridoids, Secoiridoids and Their Bioactivity. An Updated Review, Part 3 . Chem. Pharm. Bull. 57(8): 765- 796.
  • Dobler S, Petschenka G, Pankoke H (2011) Coping with toxic plant compounds – The insect’s perspective on iridoid glycosides and cardenolides. Phytochemistry, 72: 1593- 1604.
  • Rosenthal G. A, Berenbaum M. R (1991) İridoit Glikozitler, Herbivors: Their Interactions with Secondary Plant Metabolites, San Diego, California, 297- 322.
  • Skim F, Kaaya A, Jaouhari J.T, Lazrek H.B, Jana M, El Amri H (1999) Hypoglycaemic activity of Globularia alypum leaves in rats. Fitoterapia, 70: 382- 389.
  • Skim F, Lazrek H B, Kaaya A, El Amri H, Jana M (1999) Pharmacological studies of two antidiabetic plants: Globularia alypum and Zygophyllum gaetulum. Therapie, 54(6): 711-715.
  • Jouad H, Maghrani M, Eddouks M (2002) Hypoglycaemic effect of Rubus fructicosis L. and Globularia alypum L. in normal and streptozotocin- induced diabetic rats. Journal of Ethnopharmacology, 81: 351- 356.
  • Bello R, Moreno L, Primo-Yşfera E, Esplugues J (2002) Globularia alypum L. extracts reduced histamine and serotonin contraction in vitro. Phytotherapy Research, 16: 389–392.
  • Kırmızıbekmez H (1999) Globularia trichosantha fisch. ve mey: üzerinde fitokimyasal araştırmalar (Yüksek Lisans Tezi), Hacettepe Üniversitesi Eczacılık Fakültesi.
  • Boutiti A, Benguerba A, Kitouni R, Bouhroum M, Benayache S, Benayache F (2008) Secondary Metabolites From Globularia alypum. Chemistry of Natural Compounds, Vol. 44, No.
  • Taleb-Dida N, Krouf D, Bouchenak M (2011) Globularia alypum aqueous extract decreases hypertriglyceridemia and ameliorates oxidative status of the muscle, kidney, and heart in rats fed a high-fructose diet. Nutrition Research, 31: 488- 495.
  • Sesterhenn K, Distl M, Wink M (2007) Occurrence of iridoid glycosides in in vitro cultures and intact plants of Scrophularia nodosa L.. Plant Cell Rep, 26: 365- 371.
  • Sakar M. K, Tanker M (1991) İridoit Glikozitler, Fitokimyasal Analizler Tanım, Miktar Tayini ve İzolasyon, Ankara, 202- 207.
  • Bermejo P, Abad M. J, Díaz A. M, Fernández L, De Santos J, Sanchez S, Villaescus L, Carrasco L, Irurzun A (2002) Antiviral activity of seven iridoids, three saikosaponins and one phenylpropanoid glycoside extracted from Bupleurum rigidum and Scrophularia scorodonia. Planta Med. 68(2): 106
  • [18 ] Brantner A, Grein E (1994) Antibacterial activity of plant extracs used externally in traditional medicine. Journal of Ethnopharmacology, 44: 35- 40.
  • [19 ] Modaressi M, Delazar A, Nazemiyeh H, Azad F F, Smith E, Rahman M M, Gibbons S, Nahar L, Sarker S D ( 2009) Antibacterial Iridoid Glucosides from Eremostachys laciniata. Phytotherapy Research 23: 99- 103.
  • [20 ] Hsu H. Y, Yang J.J, Lin S. Y, Lin C.C (1997) Comparisons of geniposidic acid and geniposide on antitumor and radioprotection after sublethal irradiation. Cancer Letters 113: 31[21 ] Tundis R, Loizzo M.R, Menichini F, Statti G.A, Menichini F (2008) Biological and Pharmacological Activities of Iridoids: Recent Developments. Mini-Reviews in Medicinal Chemistry, 8: 399- 420.
  • [22 ] Kim S. J, Kim K. M, Park J, Kwak J. H, Kim Y. S, Lee S. M (2013) Geniposidic acid protects against D- galactosamine and lipopolysaccharide- induced hepatic failure in mice. Journal of Ethnopharmacology 146: 271- 277.
  • [23 ] Özgen U, Mavi A, Terzi Z, Coşkun M, Yıldırım A (2004) Antioxidant Activities and Total Phenolic Compounds Amount of Some Asteraceae Species. Turkish J.Pharm. Sci. 1(3): 203- 216.
  • [24 ] Çalış İ, Kırmızıbekmez H, Tasdemir D, Sticher O, Ireland C. M (2002) Sugar Esters from Globularia orientalis. Z. Naturforsch., 57c, 591D596.
  • [25 ] Khlifi S, Hachimi Y E, Khalil A, Es- Safi N, Abbouyi A E (2005) In vitro antioxidant effect of Globularia alypum L. hydromethanolic extract. Indian Pharmacol, 37: 227- 231.
  • [26 ] Harzallah H J, Neffati A, Skandrani I, Maaloul E, Chekir-Ghedira L, Mahjoub T (2010) Antioxidant and antigenotoxic activities of Globularia alypum leaves extracts. Journal of Medicinal Plants Research, 4(19): 2048- 2053.
  • [27 ] Chograni H, Riahi L, Zaouali Y, Boussaid M (2012) Polyphenols, flavonoids, antioxidant activity in leaves and flowers of Tunisian Globularia alypum L. (Globulariaceae). African Journal of Ecology, DOI: 10.1111/aje.12041.
  • [28 ] Pierik R. L. M (1997) İridoit Glikozitler, In Vitro Culture of Higher Plants, Netherlands,
  • [29 ] Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plantarum, 15: 473-497.
  • ] Gamborg O. L, Miller R. A, Ojima K (1968) Nutrition requirements of suspension cultures of soyabean root cells. Exp. Cell. Res., 50: 151- 159.
  • [31 ] Linsmaier E. M, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant., 18: 100- 127.
  • [32 ] White F. F (1993) Vectors for gene transfer in higher plants. In: Kung S. D, Wu R (eds), Transgenic Plants, Vol. 1, engineering and Utilization, pp. 15- 48, Academic Press.
  • [33 ] Nitsch J. P, Nitsch C (1969) Haploit plants from pollen grains. Science, 163: 85- 87.
  • [34 ] Lloyd G, McCown B (1980) Commercially feasible micropropagation of mountain laural (Kalmla latlfolia) by use of shoot tip cultures. Ccmb Proc Intl Soc, 30: 421- 427.
  • [35 ] Çalış İ, Kırmızıpekmez H, Rüegger H, Sticher O (1999) Phenylethanoid Glycosides from Globularia trichosantha. J. Nat. Prod., 62: 1165-1168.
  • [36 ] Çalış İ, Kırmızıpekmez H, Sticher O (2000) Iridoid Glycosides from Globularia trichosantha. J. Nat. Prod., 64: 60-64.
  • [37 ] Ueda S, Iwahashi Y. A (1991) Production of Anti- Tumor- Promoting Iridoid Glucosides in Genipa americana and its Cell Culture. Journal of Natural Products, 54(6) 1677-1680.
  • [38 ] Premjet D, Premjet S, Lelono R. A. A, Tachibana S (2010) Callus Induction and Determination of Iridoid Glycosides from Barleria prionitis Linn Leaf Explants. Australian Journal of Basic and Applied Sciences, 4(9): 4461- 4467.
  • [39 ] Chordia M. A, Kumari R. S, Kannan R. R (2010) In vitro regeneration of plantlets from internodal callus culture of Vitex leucoxylon L. – A rare medicinal plant. Journal of Medicinal Plants Research, 4(22): 2399- 2403.
  • [40 ] Yamamoto H, Katano N, Ooi A, Inoue K (1991) Transformation of loganin and 7deoxyloganin into secologanin by Lonicera japonica cell suspension cultures. Phytochemistry, 50: 417- 422.
  • [41 ] Contin A, Heijden R, Verpoorte R (1999) Accumulation of loganin and secologanin in vacuoles from suspension cultured Catharanthus roseus cells. Plant Science, 147: 177- 183.
  • [42 ] Katano N, Yamamoto H, Lio R, Inoue K (2001) 7-Deoxyloganin 7-hydroxylase in Lonicera japonica cell cultures. Phytochemistry, 58: 53- 58.
  • [43 ] Sagare A. P, Kuo C. L, Chueh L. S, Tsay H. S (2001) De Novo Regeneration of Scrophularia yoshimurae YAMAZAKI (Scrophulariaceae) and Quantitative Analysis of Harpagoside, an Iridoid Glucoside, Formed in Aerial and Underground Parts of In Vitro Propagated and Wild Plants by HPLC. Biol. Pharm. Bull., 24(11): 1311- 1315
  • [44 ] Hayta S, Akgun İ. H, Ganzera M, Bedir E, Gurel A (2011) Shoot proliferation and HPLC-determination of iridoid glycosides in clones of Gentiana cruciata L. Plant Cell Tiss Organ Cult., 107: 175- 180.
  • ] Amoo S. O, Aremu A. O, Staden J. V (2012) Shoot proliferation and rooting treatments influence secondary metabolite production and antioxidant activity in tissue culture-derived Aloe arborescens grown ex vitro. Plant Growth Regul, DOI 10.1007/s10725-013-9783-x.
  • [46 ] Shanks J. V, Morgan J (1999) Plant ‘hairy root’ culture. Current Opinion in Biotechnology, 10: 151– 155.
  • [47 ] Hwang S. J (2005) Growth Characteristics and Catalpol Production in Chinese Foxglove (Rehmannia glutinosa Liboschitz) Hairy Roots Transformed with Agrobacterium rhizogenes ATCC15834. Journal of Plant Biology, 48(4): 380- 386.
  • [48 ] Georgiev M, Heinrich M, Kerns G, Bley T, Pavlov A (2006) Production of Iridoids and Phenolics by Transformed Harpagophytum procumbens Root Cultures. Eng. Life Sci. 6 (6): 593- 596.
  • [49 ] Zhou Y. Q, Duan H. Y, Zhou C. E, Li J. J, Gu F. P, Wang F, Zhang Z. Y, Gao Z. M (2009) Hairy Root Induction and Plant Regeneration of Rehmannia glutinosa Libosch. f. Hueichingensis Hsiao via Agrobacterium rhizogenes- Mediated Transformation. Journal of Plant Physiology, 56: 2, 224-231.
  • [50 ] Grabkowska R, Krolicka A, Mielicki W, Wielanek M, Wysokinska H (2010) Genetic transformation of Harpagophytum procumbens by Agrobacterium rhizogenes: iridoid and phenylethanoid glycoside accumulation in hairy root cultures. Acta Physiol Plant, 32: 6656
  • [51 ] Piatczak E, Krolicka A, Wielanek M, Wysokinska H (2012) Hairy root cultures of Rehmannia glutinosa and production of iridoid and phenylethanoid glycosides. Acta Physiol Plant, DOI 10. 1007/ s11738-012-1022-y.
  • Müller J. L, Georgiev M, Bley T (2008) Metabolite and hormonal status of hairy root cultures of Devil’s claw (Harpagophytum procumbens) in flasks and in a bubble column bioreactor. Process Biochemistry 43: 15- 23.
  • Wu H. K, Chuang W. C, Sheu S. J (1998) Separation of nine iridoids by capillary electrophoresis and highperformance liquid chromatography. Journal of Chromatography A, 803: 179- 187.

Bitki Doku Kültüründe İridoit Glikozitler

Yıl 2013, Cilt: 25 Sayı: 3, 115 - 133, 23.12.2013
https://doi.org/10.7240/201332363

Öz

İridoit glikozitler izoprenoit (terpen) biyosentez yolu ürünleri olup, monoterpen türevleridir. Birçok farklı dikotil bitki familyasında doğal olarak sentezlenirler, ayrıca bitkileri biyotik saldırılardan ve abiyotik etkilerden korurlar. İridoit glikozitler tıbbi açıdan önemli bir yere sahiptir. İnsan sağlığı üzerinde antimikrobiyal, antitümor, antikardiyak, antienflamatuar, antihepatoma, antioksidan, nöron koruyucu gibi etkiler gösterir.

 

İridoit glikozitler bitkiler ve tıbbi açıdan büyük bir öneme sahiptir. Bu nedenle bitki doku kültürü yöntemleri ile üretimlerinin arttırılması bugün en güncel konular arasında yer almaktadır. İridoit glikozitler bitki doku kültürü yöntemleri ile üretildikten sonra ekstrakte edilerek çeşitli analiz cihazları ile kantitatif ve kalitatif analizleri yapılabilir. 

Kaynakça

  • Babaoğlu M, Gürel E ve Özcan S (2001) Bitki Biyoteknolojisi I. Doku Kültürü ve Uygulamaları. Selçuk Üniversitesi Basımevi, 374.
  • Tasdemir D, Scapozza L, Zerbe O, Linden A, Çalis I, Sticher O ( 1999) Iridoid Glycosides of Leonurus persicus. J. Nat. Prod., 62: 811- 816.
  • Crişan G, Vlase L, Balica G, Muntean D, Ştefanescu C, Paltinean R, Tamaş M, Leucuta S (2010) LC /MS Analysis of Aucubin and Catalpol of Some Veronica Species. Farmacia, 58: 237- 247.
  • Willinger G, Dobler D (2001) Selective sequestration of iridoid glycosides from their host plants in Longitarsus flea beetles. Biochemical Systematics and Ecology, 29: 335- 346.
  • Dinda B, Roy Chowdhury R, Mohanta B C (2009) Naturally Occurring Iridoids, Secoiridoids and Their Bioactivity. An Updated Review, Part 3 . Chem. Pharm. Bull. 57(8): 765- 796.
  • Dobler S, Petschenka G, Pankoke H (2011) Coping with toxic plant compounds – The insect’s perspective on iridoid glycosides and cardenolides. Phytochemistry, 72: 1593- 1604.
  • Rosenthal G. A, Berenbaum M. R (1991) İridoit Glikozitler, Herbivors: Their Interactions with Secondary Plant Metabolites, San Diego, California, 297- 322.
  • Skim F, Kaaya A, Jaouhari J.T, Lazrek H.B, Jana M, El Amri H (1999) Hypoglycaemic activity of Globularia alypum leaves in rats. Fitoterapia, 70: 382- 389.
  • Skim F, Lazrek H B, Kaaya A, El Amri H, Jana M (1999) Pharmacological studies of two antidiabetic plants: Globularia alypum and Zygophyllum gaetulum. Therapie, 54(6): 711-715.
  • Jouad H, Maghrani M, Eddouks M (2002) Hypoglycaemic effect of Rubus fructicosis L. and Globularia alypum L. in normal and streptozotocin- induced diabetic rats. Journal of Ethnopharmacology, 81: 351- 356.
  • Bello R, Moreno L, Primo-Yşfera E, Esplugues J (2002) Globularia alypum L. extracts reduced histamine and serotonin contraction in vitro. Phytotherapy Research, 16: 389–392.
  • Kırmızıbekmez H (1999) Globularia trichosantha fisch. ve mey: üzerinde fitokimyasal araştırmalar (Yüksek Lisans Tezi), Hacettepe Üniversitesi Eczacılık Fakültesi.
  • Boutiti A, Benguerba A, Kitouni R, Bouhroum M, Benayache S, Benayache F (2008) Secondary Metabolites From Globularia alypum. Chemistry of Natural Compounds, Vol. 44, No.
  • Taleb-Dida N, Krouf D, Bouchenak M (2011) Globularia alypum aqueous extract decreases hypertriglyceridemia and ameliorates oxidative status of the muscle, kidney, and heart in rats fed a high-fructose diet. Nutrition Research, 31: 488- 495.
  • Sesterhenn K, Distl M, Wink M (2007) Occurrence of iridoid glycosides in in vitro cultures and intact plants of Scrophularia nodosa L.. Plant Cell Rep, 26: 365- 371.
  • Sakar M. K, Tanker M (1991) İridoit Glikozitler, Fitokimyasal Analizler Tanım, Miktar Tayini ve İzolasyon, Ankara, 202- 207.
  • Bermejo P, Abad M. J, Díaz A. M, Fernández L, De Santos J, Sanchez S, Villaescus L, Carrasco L, Irurzun A (2002) Antiviral activity of seven iridoids, three saikosaponins and one phenylpropanoid glycoside extracted from Bupleurum rigidum and Scrophularia scorodonia. Planta Med. 68(2): 106
  • [18 ] Brantner A, Grein E (1994) Antibacterial activity of plant extracs used externally in traditional medicine. Journal of Ethnopharmacology, 44: 35- 40.
  • [19 ] Modaressi M, Delazar A, Nazemiyeh H, Azad F F, Smith E, Rahman M M, Gibbons S, Nahar L, Sarker S D ( 2009) Antibacterial Iridoid Glucosides from Eremostachys laciniata. Phytotherapy Research 23: 99- 103.
  • [20 ] Hsu H. Y, Yang J.J, Lin S. Y, Lin C.C (1997) Comparisons of geniposidic acid and geniposide on antitumor and radioprotection after sublethal irradiation. Cancer Letters 113: 31[21 ] Tundis R, Loizzo M.R, Menichini F, Statti G.A, Menichini F (2008) Biological and Pharmacological Activities of Iridoids: Recent Developments. Mini-Reviews in Medicinal Chemistry, 8: 399- 420.
  • [22 ] Kim S. J, Kim K. M, Park J, Kwak J. H, Kim Y. S, Lee S. M (2013) Geniposidic acid protects against D- galactosamine and lipopolysaccharide- induced hepatic failure in mice. Journal of Ethnopharmacology 146: 271- 277.
  • [23 ] Özgen U, Mavi A, Terzi Z, Coşkun M, Yıldırım A (2004) Antioxidant Activities and Total Phenolic Compounds Amount of Some Asteraceae Species. Turkish J.Pharm. Sci. 1(3): 203- 216.
  • [24 ] Çalış İ, Kırmızıbekmez H, Tasdemir D, Sticher O, Ireland C. M (2002) Sugar Esters from Globularia orientalis. Z. Naturforsch., 57c, 591D596.
  • [25 ] Khlifi S, Hachimi Y E, Khalil A, Es- Safi N, Abbouyi A E (2005) In vitro antioxidant effect of Globularia alypum L. hydromethanolic extract. Indian Pharmacol, 37: 227- 231.
  • [26 ] Harzallah H J, Neffati A, Skandrani I, Maaloul E, Chekir-Ghedira L, Mahjoub T (2010) Antioxidant and antigenotoxic activities of Globularia alypum leaves extracts. Journal of Medicinal Plants Research, 4(19): 2048- 2053.
  • [27 ] Chograni H, Riahi L, Zaouali Y, Boussaid M (2012) Polyphenols, flavonoids, antioxidant activity in leaves and flowers of Tunisian Globularia alypum L. (Globulariaceae). African Journal of Ecology, DOI: 10.1111/aje.12041.
  • [28 ] Pierik R. L. M (1997) İridoit Glikozitler, In Vitro Culture of Higher Plants, Netherlands,
  • [29 ] Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plantarum, 15: 473-497.
  • ] Gamborg O. L, Miller R. A, Ojima K (1968) Nutrition requirements of suspension cultures of soyabean root cells. Exp. Cell. Res., 50: 151- 159.
  • [31 ] Linsmaier E. M, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant., 18: 100- 127.
  • [32 ] White F. F (1993) Vectors for gene transfer in higher plants. In: Kung S. D, Wu R (eds), Transgenic Plants, Vol. 1, engineering and Utilization, pp. 15- 48, Academic Press.
  • [33 ] Nitsch J. P, Nitsch C (1969) Haploit plants from pollen grains. Science, 163: 85- 87.
  • [34 ] Lloyd G, McCown B (1980) Commercially feasible micropropagation of mountain laural (Kalmla latlfolia) by use of shoot tip cultures. Ccmb Proc Intl Soc, 30: 421- 427.
  • [35 ] Çalış İ, Kırmızıpekmez H, Rüegger H, Sticher O (1999) Phenylethanoid Glycosides from Globularia trichosantha. J. Nat. Prod., 62: 1165-1168.
  • [36 ] Çalış İ, Kırmızıpekmez H, Sticher O (2000) Iridoid Glycosides from Globularia trichosantha. J. Nat. Prod., 64: 60-64.
  • [37 ] Ueda S, Iwahashi Y. A (1991) Production of Anti- Tumor- Promoting Iridoid Glucosides in Genipa americana and its Cell Culture. Journal of Natural Products, 54(6) 1677-1680.
  • [38 ] Premjet D, Premjet S, Lelono R. A. A, Tachibana S (2010) Callus Induction and Determination of Iridoid Glycosides from Barleria prionitis Linn Leaf Explants. Australian Journal of Basic and Applied Sciences, 4(9): 4461- 4467.
  • [39 ] Chordia M. A, Kumari R. S, Kannan R. R (2010) In vitro regeneration of plantlets from internodal callus culture of Vitex leucoxylon L. – A rare medicinal plant. Journal of Medicinal Plants Research, 4(22): 2399- 2403.
  • [40 ] Yamamoto H, Katano N, Ooi A, Inoue K (1991) Transformation of loganin and 7deoxyloganin into secologanin by Lonicera japonica cell suspension cultures. Phytochemistry, 50: 417- 422.
  • [41 ] Contin A, Heijden R, Verpoorte R (1999) Accumulation of loganin and secologanin in vacuoles from suspension cultured Catharanthus roseus cells. Plant Science, 147: 177- 183.
  • [42 ] Katano N, Yamamoto H, Lio R, Inoue K (2001) 7-Deoxyloganin 7-hydroxylase in Lonicera japonica cell cultures. Phytochemistry, 58: 53- 58.
  • [43 ] Sagare A. P, Kuo C. L, Chueh L. S, Tsay H. S (2001) De Novo Regeneration of Scrophularia yoshimurae YAMAZAKI (Scrophulariaceae) and Quantitative Analysis of Harpagoside, an Iridoid Glucoside, Formed in Aerial and Underground Parts of In Vitro Propagated and Wild Plants by HPLC. Biol. Pharm. Bull., 24(11): 1311- 1315
  • [44 ] Hayta S, Akgun İ. H, Ganzera M, Bedir E, Gurel A (2011) Shoot proliferation and HPLC-determination of iridoid glycosides in clones of Gentiana cruciata L. Plant Cell Tiss Organ Cult., 107: 175- 180.
  • ] Amoo S. O, Aremu A. O, Staden J. V (2012) Shoot proliferation and rooting treatments influence secondary metabolite production and antioxidant activity in tissue culture-derived Aloe arborescens grown ex vitro. Plant Growth Regul, DOI 10.1007/s10725-013-9783-x.
  • [46 ] Shanks J. V, Morgan J (1999) Plant ‘hairy root’ culture. Current Opinion in Biotechnology, 10: 151– 155.
  • [47 ] Hwang S. J (2005) Growth Characteristics and Catalpol Production in Chinese Foxglove (Rehmannia glutinosa Liboschitz) Hairy Roots Transformed with Agrobacterium rhizogenes ATCC15834. Journal of Plant Biology, 48(4): 380- 386.
  • [48 ] Georgiev M, Heinrich M, Kerns G, Bley T, Pavlov A (2006) Production of Iridoids and Phenolics by Transformed Harpagophytum procumbens Root Cultures. Eng. Life Sci. 6 (6): 593- 596.
  • [49 ] Zhou Y. Q, Duan H. Y, Zhou C. E, Li J. J, Gu F. P, Wang F, Zhang Z. Y, Gao Z. M (2009) Hairy Root Induction and Plant Regeneration of Rehmannia glutinosa Libosch. f. Hueichingensis Hsiao via Agrobacterium rhizogenes- Mediated Transformation. Journal of Plant Physiology, 56: 2, 224-231.
  • [50 ] Grabkowska R, Krolicka A, Mielicki W, Wielanek M, Wysokinska H (2010) Genetic transformation of Harpagophytum procumbens by Agrobacterium rhizogenes: iridoid and phenylethanoid glycoside accumulation in hairy root cultures. Acta Physiol Plant, 32: 6656
  • [51 ] Piatczak E, Krolicka A, Wielanek M, Wysokinska H (2012) Hairy root cultures of Rehmannia glutinosa and production of iridoid and phenylethanoid glycosides. Acta Physiol Plant, DOI 10. 1007/ s11738-012-1022-y.
  • Müller J. L, Georgiev M, Bley T (2008) Metabolite and hormonal status of hairy root cultures of Devil’s claw (Harpagophytum procumbens) in flasks and in a bubble column bioreactor. Process Biochemistry 43: 15- 23.
  • Wu H. K, Chuang W. C, Sheu S. J (1998) Separation of nine iridoids by capillary electrophoresis and highperformance liquid chromatography. Journal of Chromatography A, 803: 179- 187.
Toplam 52 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Araştırma Makaleleri
Yazarlar

Havva Atar Bu kişi benim

Hatice Çölgeçen

Yayımlanma Tarihi 23 Aralık 2013
Yayımlandığı Sayı Yıl 2013 Cilt: 25 Sayı: 3

Kaynak Göster

APA Atar, H., & Çölgeçen, H. (2013). Bitki Doku Kültüründe İridoit Glikozitler. Marmara Fen Bilimleri Dergisi, 25(3), 115-133. https://doi.org/10.7240/201332363
AMA Atar H, Çölgeçen H. Bitki Doku Kültüründe İridoit Glikozitler. MFBD. Aralık 2013;25(3):115-133. doi:10.7240/201332363
Chicago Atar, Havva, ve Hatice Çölgeçen. “Bitki Doku Kültüründe İridoit Glikozitler”. Marmara Fen Bilimleri Dergisi 25, sy. 3 (Aralık 2013): 115-33. https://doi.org/10.7240/201332363.
EndNote Atar H, Çölgeçen H (01 Aralık 2013) Bitki Doku Kültüründe İridoit Glikozitler. Marmara Fen Bilimleri Dergisi 25 3 115–133.
IEEE H. Atar ve H. Çölgeçen, “Bitki Doku Kültüründe İridoit Glikozitler”, MFBD, c. 25, sy. 3, ss. 115–133, 2013, doi: 10.7240/201332363.
ISNAD Atar, Havva - Çölgeçen, Hatice. “Bitki Doku Kültüründe İridoit Glikozitler”. Marmara Fen Bilimleri Dergisi 25/3 (Aralık 2013), 115-133. https://doi.org/10.7240/201332363.
JAMA Atar H, Çölgeçen H. Bitki Doku Kültüründe İridoit Glikozitler. MFBD. 2013;25:115–133.
MLA Atar, Havva ve Hatice Çölgeçen. “Bitki Doku Kültüründe İridoit Glikozitler”. Marmara Fen Bilimleri Dergisi, c. 25, sy. 3, 2013, ss. 115-33, doi:10.7240/201332363.
Vancouver Atar H, Çölgeçen H. Bitki Doku Kültüründe İridoit Glikozitler. MFBD. 2013;25(3):115-33.

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