Investigation of MBL-1 gene polymorphism in some cattle breeds raised in Turkey

Abstract

The concern in using genetic methods in forming resistant herds against diseases in farm animals has recently been increasing. Mannose-binding lectin-1 (MBL-1) gene is one of the genes searched in this kind of research because of this gene's important responsibilities in the immune system. In the presented research, Zavot (n= 81, Z), Anatolian Black (n= 87, AB), Eastern Anatolian Red (n= 72, EAR), Turkish Gray (n= 54, TG), Southern Anatolian Red (n= 44, SAR) which are the cattle breeds raised in Turkey, and Black Switzerland (n= 61, BS) and Simmental (n= 65, S) which are Europe originated cattle breeds were used as the research material. In the research, it was aimed at genotyping the samples of cattle searched in terms of total three single nucleotide polymorphism in the intron 1 (1252 G>A) and exon 2 (2534 G>A, 2569 T>C) regions of MBL-1 gene. In the end of the study, it was found that Hardy Weinberg (HWE) equilibrium broke down in EAR and S breeds (p<0.05) in terms of 1252 G>A coded SNP; in NB and BS races (p<0.001 and p<0.01 respectively) in terms of 2534G>A coded SNP. It was determined that all breeds are in HWE in terms of 2569 T>C coded SNP. This research is the first research where SNPs in intron 1 (1252 G>A) and exon 2 (2534 G>A, 2569 T>C) regions of MBL-1 gene in the cattle breeds raised in Turkey have been searched. At the end of the study, it was thought that the studies where the relationships between SNPs and important raising disease are searched should be planned.

Keywords

• MBL-1,Cattle,PCR-RFLP,SNP

Türkiye’de yetiştirilen bazı sığır ırklarında MBL-1 gen polimorfizminin araştırılması

Abstract

Çiftlik hayvanlarında son yıllarda hastalıklara karşı daha dirençli sürülerin oluşturulması için genetik yöntemlerin kullanılmasına yönelik ilgi giderek artmaktadır. Bu tür çalışmalarda immun sistemde önemli görevlerinden dolayı mannoz bağlayıcı lektin-1 (MBL-1) geni de incelenen genlerden biridir. Sunulan çalışmada araştırma materyali olarak Türkiye’de yetiştirilen yerli sığır ırklarından Zavot (n= 81, Z), Yerli Kara (n= 87, YK), Doğu Anadolu Kırmızısı (n= 72, DAK), Boz ırk (n= 54, BI), Güney Anadolu Kırmızısı (n= 44, GAK) ile Avrupa orijinli sığır ırklarından İsviçre Esmeri (n= 61, BS) ve Simental (n= 65, S) ırkı sığırlar kullanılmıştır. Çalışmada MBL-1 geninin intron 1 (1252 G>A) ve ekzon 2 bölgelerinde (2534 G>A, 2569 T>C) bulunan toplam üç tek nükleotid polimorfizmi (SNP) yönünden incelenen sığır ırklarına ait örneklerin genotiplendirilmesi hedeflenmiştir. Çalışma sonunda 1252 G>A kodlu SNP yönünden DAK ve S ırklarında (p<0.05); 2534G>A kodlu SNP yönünden YK ve BS ırklarında (p<0.001, p<0.01 sırasıyla) Hardy Weinberg (HWE) dengesinin bozulduğu görülmüştür. 2569 T>C kodlu SNP yönünden ise tüm ırkların HW dengesinde oldukları belirlenmiştir. Bu çalışma Türkiye’de yetiştirilen sığır ırklarında MBL-1 geninin intron 1 (1252 G>A) ve ekzon 2 bölgelerinde (2534 G>A, 2569 T>C) bulunan SNP’lerin araştırıldığı ilk çalışmadır. Çalışma sonunda bu SNP’ler ile önemli yetiştiricilik hastalıkları arasındaki ilişkilerin araştırıldığı çalışmaların planlanması gerektiği düşünülmüştür.

Keywords

• MBL-1,PCR-RFLP,SNP,Sığır

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