Domateste Tomato spotted wilt virüs’üne karşı dayanıklılığı kıran izolatının fenotipik karakterizasyonu

Year 2019, , 307 - 314, 01.12.2019

Hakan Fidan , Nuray Sarı

https://doi.org/10.29136/mediterranean.596401

Cited By: 3

Abstract

Domateste lekeli solgunluk virüsü (Tomato
spotted wilt virus-TSWV), dünya genelinde ekonomik anlamda büyük kayıplar
meydana getiren virüslerden biridir. Son zamanlara kadar domateslerde Sw-5
geni vasıtasıyla bu hastalık ile mücadele edilmekteydi. 2016-2019 yılları
arasında Antalya ili ve ilçelerinde domates yetiştiriciliği yapılan seralarda Sw-5
geni barındıran çeşitler üzerinde TSWV’a ait simptomların geliştiği
gözlemlenmiştir. Bu izolatların PCR çalışmaları ile bitkilerin Sw-5 geni
içerdiği ve RT-PCR (Revers-Transkriptaz Polimeraz Zincir Reaksiyonu)
çalışmaları ile de simptomların TSWV’a ait olduğu doğrulandıktan sonra izolatın
ismi TSWVAntRB olarak belirlenmiştir. Yapılan gözlemlemeler sonucunda TSWVAntRB
izolatının farklı simptomolojik karakterlere sahip olduğu tespit edilmiştir. Bu
farklılıklarının belirlenebilmesi için TSWVAntRB izolatının, bitkiler üzerine
mekanik inokulasyonu 5 tekerrürlü olacak şekilde gerçekleştirilmiştir. Mekanik
inokulasyon çalışmalarında; Sw-5 geni içeren 4 adet ticari çeşit, bir
adet dayanıklılık kaynağı olarak bilinen Solanum peruvianum ve bir adet
hassas çeşit kullanılmıştır. Sw-5 dayanımını kırmayan (NRB) izolatında
şimdiye kadar yaprakta sadece nekrotik lekelenmeler meydana gelirken, Sw-5
dayanımını kıran (RB) izolatında yaprak üzerinde de halkalı lekelenmelerin
meydana geldiği belirlenmiştir. Aynı zamanda bazı çeşitler üzerinde ilk
simptomlar meyve dönemine kadar gizli kaldığı ve meyve salkımında bazen bir
veya iki meyve üzerinde simptomlar meydana getirdiği gözlemlenmiştir. Çalışmada
simptom şiddetinin hava sıcaklığı ile ilişkili olduğu saptanmış ve TSWVAntRB
izolatının simptomolojik özellikleri her açıdan incelenmiştir.

Keywords

Dayanıklılığın kırılması, Domates, Domates lekeli lolgunluk virüsü (TSWV), Mekanik inokulasyon, RT-PCR

Supporting Institution

Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

FYL-2017-2619

Thanks

Bu çalışma Akdeniz Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından FYL-2017-2619 nolu proje ile desteklenmiştir.

Phenotypic characterization of Tomato spotted wilt virus resistance breaking isolate in tomatoes

Abstract

Tomato spotted wilt virus (TSWV) is one of the most important viruses that
cause great economic losses in worldwide. Tomatoes including Sw-5 resistance gene control resistance
but they have recently been struggling with the virus disease. It was observed
that typical symptoms developed on the varieties containing Sw-5 gene in tomato cultivation areas in
Antalya province and its districts between 2016-2019 years. These isolates were
identified as TSWVAntRB after PCR studies and their host tomato plants were
contained the Sw-5 gene in RT-PCR
analyses confirming that their symptoms belong to TSWVAntRB isolates. Further,
observations revealed that TSWVAntRB isolate had different symptomological
characteristics which explaining the differences. Mechanical inoculations with
TSWVAntRB isolate on plants were performed with 5 repeats where 4 commercial
varieties containing Sw-5 gene, a Solanum peruvianum plant known as
resistance source and one susceptible variety were used. Their results revealed
that only necrotic spotting occurred in the leaves within the Sw-5 non-resistance breaking (NRB)
isolate while typical cyclic spotting presented within the Sw-5 resistance breaking (RB) isolates. Additionally, first
symptoms remained hidden until the fruit period and sometimes symptoms obtained
on one or two fruits in a bunch. It was found that the severity of the symptoms
increased within parallel to air temperature. We have been evaluating TSWVAntRB
isolates’ symptomological features in every aspect.

Keywords

Resistance breaking, Tomato, Tomato spotted wilt virus (TSWV), Mechanical inoculation, RT-PCR

Project Number

FYL-2017-2619

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