Adana ve Mersin illerinde (Türkiye) yetiştirilen marullarda (Lactuca sativa) marul iri damar hastalığının tanılanması ve karakterizasyonu

Abstract

Bu çalışma, Marul iri damar hastalığı (Lettuce big-vein disease, LBVD)’nın saptanması, bu hastalığa neden olan virüs veya virüs kompleksinin (MiLBVV ve/veya LBVaV) belirlenmesi  ve bu etmenlerin karakterizasyonu amacıyla, 2015-2017 yılları arasında sonbahar ve kış aylarında, Adana ve Mersin illerinde yaygın olarak marul yetiştiriciliğinin yapıldığı alanlarda, yürütülmüştür. Arazi çıkışlarında, simptomatolojik olarak LBVD ile enfekteli olduğundan şüphelenilen toplam 160 adet marul bitkisinden örnekleme yapılmıştır. ELISA testleri sonucunda, 52 marul örneği MiLBVV ile enfekteli bulunmuştur. LBVD’ye sebep olan etmenlerin belirlenmesi amacıyla, MiLBVV (MiLBVV-F; MiLBVV-R) ve LBVaV (VP-248;VP-249)’ye spesifik primer çiftleri kullanılarak yapılan RT-PCR çalışmaları sonucunda, MiLBVV veya MiLBVV+LBVaV karışık enfeksiyonunun varlığı ortaya konmuştur. LBVD simptomu gösteren marul bitkilerinde tek başına LBVaV enfeksiyonu saptanmamıştır. Sekans analizlerinde, MiLBVV’nin Adana ve Mersin izolatları filogenetik ağaç üzerinde kendi aralarında ayrı birer grup oluşturmuşlar ve Adana ilinden, Yakapınar (2-MilBVV 1 E10), Yumurtalık (6-MiLBVV 1 G10) ve Yüreğir (4-MiLBVV 1 F10) izolatları, Arjantin ve İran izolatları ile birlikte yer alırken, Mersin ilinden Yenice 1 (11-MiLBVV 2 H10) ve Yenice 2 (12-MiLBVV 2 E11) izolatları, Hollanda ve Mısır izolatları ile aynı grupta yer almışlardır. Bunun yanında, LBVaV’nın Yüreğir izolatı (4-VP 248 A12), Suudi Arabistan, ABD, Birleşik Krallık, Avustralya ve Hollanda izolatları ile grup oluşturmuştur.

Keywords

• Marul,LBVD,DAS-ELISA,RT-PCR,Sekans analizi

Project Number

FYL-2016-7227

Identification and characterization of lettuce big vein disease (LBVD) in lettuce (Lactuca sativa) crops in Adana and Mersin provinces in Turkey

Abstract

This study was carried out for the purpose of determination of Lettuce big vein disease (LBVD), identification of the virus or virus complex (MiLBVV and/or LBVaV) causing disease and characterization of causal agent in the fields where the lettuce cultivation was made extensively in Adana and Mersin provinces during autumn and winter months between 2015 and 2017. A total of 160 samples were collected from lettuce (Lactuca sativa L.) plants suspected to be infected with LBVD simptomatologically in surveys. In the result of the ELISA tests, 52 samples were found to be infected with MiLBVV. RT-PCR studies were performed to identify the causative agents of LBVD by using specific primer pairs for MiLBVV (MiLBV-F; MiLBVV-R) and LBVaV (VP-248; VP-249) showed that LBVD is caused by MiLBVV or mixed infection of MiLBVV+LBVaV. LBVaV infection was not detected alone in lettuce plants with LBVD symptoms. Sequence analyses showed that Adana and Mersin isolates of MiLBVV formed in separate groups on phylogenetic tree. While Yakapınar (2-MiLBVV 1 E10), Yumurtalık (6-MiLBVV 1 G10) and Yüreğir (4-MiLBVV 1 F10) isolates from Adana province clustered with Argentina and Iran isolates, Yenice 1 (11-MiLBVV 2 H10) and Yenice 2 (12-MiLBVV 2 E11) isolates from Mersin province were in the same group with Netherlands and Egypt isolates in another group. In addition, the Yüreğir isolate of LBVaV (4-VP 248 A12) clustered with isolates from Saudi Arabia, United States, United Kingdom, Australia and Netherlands.

Keywords

• Lettuce,LBVD,DAS-ELISA,RT-PCR,Sequence analysis

Supporting Institution

BAP Unit of Cukurova University

Project Number

FYL-2016-7227

Thanks

We would like to thank the Çukurova University Research Fund for their financial support throughout the study

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