Determination of SacII and MboII polymorphisms in the Nerve Growth Factor (NGF) gene in four native Turkish goat populations

Abstract

Nerve Growth Factor Gene (NGF) has important functions in the maintenance of viability and proliferation of ovarian cells. Recent studies on caprine NGF gene revealed two SNPs (determined by SacII/C291A and MboII/A705G polymorphisms) related with body length and litter size in goats. This study aimed to determine SacII and MboII polymorphisms of NGF in native Turkish goat populations including Turkish Hair (HAI), Kabakulak (KBK), Honamlı (HNM) and Norduz (NRD) by using PCR-RFLP. Amplificated PCR fragments of 808 were digested with SacII, while amplificated PCR fragments of 319 bp were digested with MboII restriction enzyme in order to detect different genotypes of NGF gene. The “A” allele frequency ranged from 0.500 (HAI, HNM and KBK) to 0.950 (NRD), while the “C” allele frequency ranged from 0.025 (NRD) to 0.500 (HAI, HNM and KBK) in NGF/SacII polymorphism. The “A” allele frequency ranged from 0.213 (HAI) to 1.000 (NRD), while the “G” allele frequency ranged from 0.000 (NRD) to 0.787 (HAI) in NGF/MboII polymorphism. Deviation from HW equilibrium was significant in HNM goat population (P < 0.05). In this study, polymorphisms of caprine NGF gene in native Turkish goat populations were revealed for the first time. The results obtained from this study showed that NGF/SacII polymorphisms could be used for body length in NRD population while NGF/MboII polymorphisms could be used for litter size in HAI, HNM and KBK populations in MAS studies.

Keywords

• Body length,Litter size,Nerve Growth Factor,NGF,PCR-RFLP

Türkiye’de yetiştirilen dört keçi populasyonunda Sinir Büyüme Faktörü (NGF) geninde SacII ve MboII polimorfizmlerinin belirlenmesi

Abstract

Sinir Büyüme Faktörü (NGF) geni yumurtalık hücrelerinin yaşamını devam ettirmesi ve çoğalmasında önemli fonksiyonlara sahiptir. Keçi NGF geni üzerinde yapılan son çalışmalar, iki SNP’in (SacII/C291A ve MboII/A705G polimorfizmi) vücut uzunluğu ve bir batında doğan yavru sayısı ile ilişkili olduğunu ortaya çıkarmıştır. Bu çalışmada, Türkiye’de yetiştirilen Kıl (HAI), Kabakulak (KBK), Honamlı (HNM) ve Norduz (NRD) keçi populasyonlarının NGF geninde SacII ve MboII polimorfizminin PCR-RFLP yöntemiyle belirlenmesi amaçlanmıştır. NGF geninde farklı genotiplerin belirlenmesi için çoğaltılan 808 bç uzunluğundaki PCR ürünleri SacII, 319 bç uzunluğundaki PCR ürünleri ise MboII restriksiyon enzimi ile kesilmiştir. NGF/SacII polimorfizminde A allel frekansı 0.500 (Kıl, Honamlı ve Kabakulak) ile 0.950 (Norduz) aralığında değişirken, C allel frekansı 0.025 (Norduz) ile 0.500 (Kıl, Honamlı ve Kabakulak) aralığında değişmiştir. NGF/MboII polimorfizminde A allel frekansı 0.213 (Kıl) ile 1.000 (Norduz) aralığında değişirken, G allel frekansı 0.000 (Norduz) ile 0.787 (Kıl) aralığında değişmiştir. Honamlı populasyonunda HW dengesinden sapma önemli bulunmuştur (P < 0.05). Bu çalışmada, Türkiye’nin yerli keçi populasyonlarında keçi NGF gen polimorfizmi ilk defa ortaya çıkarılmıştır. Bu çalışmandan elde edilen sonuçlar, NGF/SacII polimorfizminin Norduz popuslayonunda vücut uzunluğu, için, NGF/MboII polimorfizminin ise Kıl, Honamlı ve Kabakulak populasyonlarında bir batında doğan yavru sayısı için markör destekli seleksiyon (MAS) çalışmalarında kullanılabileceğini göstermiştir.

Keywords

• Vücut uzunluğu,Yavru sayısı,Sinir Büyüme Faktörü,NGF,PCR-RFLP

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