Biber hafif benek virüs’üne (PMMoV) karşı L4 dayanıklılık durumunun taranması ve moleküler yöntemlerle karakterizasyonu

Year 2019, Volume: 32 Issue: 3, 297 - 305, 01.12.2019

Hakan Fidan , Murat Barut

https://doi.org/10.29136/mediterranean.594474

Cited By: 3

Abstract

Biber hafif benek virüsü (Pepper mild mottle virus- PMMoV) Tobamovirüsler içerisinde yer alan dünya genelinde biber üretim
alanlarında en sık karşılaşılan virüslerden biridir. Bu virüs çoğu zaman hasat
dönemine kadar gizli kalabilmekte ve en büyük zararlarını hasat döneminde
meyveler üzerinde meydana getirebilmektedir. Bölgemizde son yıllarda biber
üretim alanlarında sıklıkla karşılaşılan bir problem olarak karşımıza
çıkmaktadır. Dünyanın farklı bölgelerinde L4 geni vasıtasıyla sağlanan
dayanıklılığı kıran virüs izolatının rapor edilmesi, ülkemizde de PMMoV’nin epidemi
durumunun araştırılması gerekliliğini ortaya koymuştur. L4 dayanıklılığının etkinliğini
belirleyebilmek için bölgemizden biber üretim alanlarında PMMoV ile şüpheli
örnekler toplanmıştır. RT-PCR tekniği ile doğrulanan bu örnek PMMoV-Kum olarak
kodlanmıştır ve Gen bankasına kaydı yapılarak erişim numarası (MK806437)
alınmıştır. PMMoV-Kum izolatı 1 hassas (B1) ve 5 dayanıklı (L4B1, L4B2, L4B3, L4B4,
L4B5) bitkilerine mekanik inokulasyon yöntemi ile bulaştırılmıştır. Yapılan
fenotipik gözlemler neticesinde PMMoV-Kum izolatının L4 genine bağlı
dayanıklılığı kıramadığı biyolojik testler ile doğrulanmıştır. Dayanıklı olarak
seçilen bitkiler üzerinde herhangi bir PMMoV simptomunun oluşmaması üzerine en
başarılı çalışan L4 markırlarını belirlemeye yönelik primerlerin belirlenmesi
tez çalışmasının seyrini belirlemiş ve en başarılı markırın AP-7/AP-8 ve
P118/119primer çiftleri olduğu tespit edilmiştir.

Keywords

L4 geni, Dayanıklılığın kırılması, Biber hafif benek virüsü, Biber, PMMoV

Supporting Institution

Akdeniz Üniversitesi Bitki Koruma Bölümü

Project Number

FYL-2018-3638

Thanks

Bu Tez Akdeniz Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiştir. Teşekkür Ederiz.

Screening of L4 resistance status to pepper mild mottle virus (PMMoV) and characterization by molecular methods

Abstract

Pepper mild mottle virus (PMMoV) is one of the
most common Tobamoviruses can be found in worldwide. This virus has
potential to cause economic losses and has kept secret infections until harvest
time causing great damages on pepper fruits at this period. During 2016-2019
production periods, it has been frequently seen in pepper grown areas. Several
isolates reported from different regions of the world regarding resistance with
L4 gene fights against this virus disease. In this study, we aimed to
investigate how the PMMoV increased and understood its incidence pepper grown
areas in Turkey. In order to determine the effectiveness of L4 resistance,
PMMoV and other suspected pepper samples were isolated. The collected samples
were studied by RT-PCR technique using PMMoV-Kum isolate and its gene bank’s
access number (MK806437) was registered. A sensitive (B1) and 5 resistant
(L4B1, L4B2, L4B3, L4B4, L4B5) plants were mechanically inoculated with the
PMMoV-Kum isolate and their phenotypic observations were recorded. The
PMMoV-Kum isolate was confirmed as avirulent in biological tests, the PMMoV
could not overcome L4 gene mediated resistance. Furthermore, a
successful primer set has determined as AP-7 / AP-8 in L4 gene mediated
resistance against occurrence of any PMMoV symptoms on the tested plants.
Determining new PMMoV isolates which break the L4 gene mediated resistance, phylogenetic analyzes was performed to
determine its place among the world’s isolates.

Keywords

L4 gene, Resistance Breaking, Pepper, Pepper mild mottle virus, PMMoV

Project Number

FYL-2018-3638

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