Investigation of the shape of goat (capra hircus) astragalus via geometric morphometry method

Yıl 2024, , 277 - 282, 31.08.2024

Tuğba Oktay , Yasin Demiraslan , Özcan Özgel

https://doi.org/10.24880/meditvetj.1541126

Öz

The aim of this study is to determine the effect of sex on shape of goat astragalus via geometric morphometry method. A total of 37 astragalus bone samples collected from 16 female and 21 male goats were used as material. Bone samples were cleaned from skin and soft tissue and then macerated by boiling. Astragalus bone samples were photographed from a distance of 20 cm dorsally by focusing on the center of the bone. The photographs were transferred to the computer in JPEG format. Principal Component Analysis, Regression Analysis, Canonical Variate Analysis, and Discriminant Function Analysis were conducted using the Cartesian coordinate values, which were obtained by homologous landmark marking. The first two principal components accounted for 75.967% of the total shape variation. Shape variation was determined in different regions of the astragalus. According to the scatter plot of male and female individuals, male individuals were completely placed within the confidence interval ellipse of female individuals. It was found that allometric effect on the shape of astragalus bone was not statistically significant. As a result of Canonical Variate Analysis, mahalanobis and procrustes distances were detected as 2.9216 (p<0.0001) and 0.0645 (p=0.0035), respectively. This test indicated two female individuals in the group of males. The proximal of male goat astragalus was wider than that of female goats. The results of the Discriminate Function Analysis revealed that 8 of the female individuals and 7 of the male individuals were incorrectly grouped according to the cross validation scores. Geometric morphometry and the related analyses allowed to examine the differences between the astragalus bone samples of male and female goats. The fact that the astragalus bone of male goats was wider than that of females supported the studies using linear measurements in the literature. Consequently, the result indicating that sex factor had a limited grouping effect on astragalus shape in goats was obtained.

Anahtar Kelimeler

astragalus, capra hircus, gender shape

Etik Beyan

According to Article 8 of the Regulation on Working Procedures and Principles of Animal Experiments Ethics Committees dated 15 February 2014 and numbered 28914, Ethics Committee Approval is not required (E-93773921-020-354464).

Kaynakça

• Aytek, A. İ. (2017). Geometrik morfometri. Mimarlar Arkeologlar Sanat Tarihçileri Restoratörler Ortak Platformu E-Dergisi, 11(17), 1-7.
• Azra, M. Abhaya Prabhu. N, Balachandra. (2020). An anatomical study on types of calcaneal facets on talus and co relation between squatting facets and angles of neck. Indian Journal of Clinical Anatomy and Physiology 5(4), 434-438. DOI: 10.18231/2394-2126.2018.0101.
• Bass, W. M. (1995). Human Osteology: A laboratory and field manual (4th ed.). Missouri Archeological Society: Columbia.
• Bidmos, M. A. and Dayal, M. R. (2004). Further evidence to show population specificity of discriminant function equations for sex determination using the talus of South African blacks. Journal of Forensic Sciences, 49(6), 1165–1170. PMID: 15568686.
• Bilge, Y. (2008). Adli Tıp. Üçbilek Matbaası: Ankara.
• Boyan, N. Ozsahin, E. Kizilkanat, E. Soames, R. Oguz, O. (2016). Morphometric measurement and types of articular facets on the talus and calcaneus in an Anatolian population. International Journal of Morphology. 34(4), 1378-1385.
• Brzobohata, H. (2015). Sex classification using the three-dimensional tibia form or shape including population specificity approach. Forensic Sciences, 60(1), 29–40. https://doi.org/10.1111/1556-4029.12641 PMID: 25387800.
• Davies, C. M., Hackman, L., Black, S. M. (2014). The foot in forensic human identification - A review. Foot, 24(1), 31–36. https://doi.org/10.1016/j.foot.2013.12.001 PMID: 24382464.
• Demiraslan, Y., Özgel, Ö., Gürbüz, İ., Zümre, Ö. (2021). The mandibles of the Honamli and Hair goat (Capra hircus); a geometric morphometric study. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 68, 321-328. DOI: 10.33988/auvfd.759964.
• Evcim, B. (2020). Koyun, keçi ve tavşanda mandibula’nın geometrik özellikleri. Yüksek Lisans Tezi, Aydın Adnan Menderes Üniversitesi Sağlık Bilimleri Enstitüsü Anatomi (Veteriner) Programı, Aydın.
• Figus, C., Traversari, M., Scalise, L. M., Oxilia, G., Vazzana, A., Buti, L., et al. (2017). The study of commingled nonadult human remains: Insights from the 16th–18th centuries community of Roccapelago (Italy). Journal of Archaeological Science: Reports, 14, 382-391. https://doi.org/10.1016/j.jasrep.2017.06.023.
• Gonzalez, P. N., Bernal. V., Perez, S. I. (2009). Geometric morphometric approach to sex estimation of human pelvis. Forensic Science International, 189 (1-3), 68-74. doi: 10.1016/j.forsciint.2009.04.012.
• Graci, V., Van Dillen, L. R., Salsich, G. B. (2012). Gender differences in trunk, pelvis and lower limb kinematics during a single leg squat. Gait Posture, 36(3), 461–466. https://doi.org/10.1016/j.gaitpost.2012.04.006 PMID: 22591790.
• Haruda, A. F. (2017). Separating sheep (ovis aries l.) and goats (capra hircus l.) using geometric morphometric methods: an investigation of astragalus morphology from late and final bronze age central asian contexts. International Journal of Osteoarchaeology, 27(4), 551–562. https://doi.org/10.1002/oa.2576.
• Jeanjean, M., Haruda, A., Salvagno, L., Schafberg, R., Valenzuela-Lamas, S., Nieto-Espinet, A., Forest, V., Blaise, E., Vuillen, M., Mureau, C., Evin, A. (2022). Sorting the flock: Quantitative identification of sheep and goat from isolated third lower molars and mandibles through geometric morphometrics. Journal of Archaeological Science, 141. https://doi.org/10.1016/j.jas.2022.105580.
• Klingenberg, C. P. (2011). MorphoJ: an integrated software package for geometric Morphometrics. Mol Ecol Resour, 11, 353-357.
• König, H. E., and Liebich, H. G. (2013). Veterinary anatomy of domestic mammals: textbook and colour atlas. (4th ed). Stuttgart, Schattauer Verlag, 197-218.
• Kranioti, E. F., Bastir, M., Sanchez-Meseguer, A., Rosas, A. (2009). A geometric-morphometric study of the cretan humerus for sex identification. Forensic Science International, 189, 2–9. https://doi.org/10.1016/j.forsciint.2009. 04.013 PMID: 19446415.
• Lee, J. Y. Jung, M. H. Lee, J. S. Choi, B. Y. Cho, B. P. (2012b). Types of calcaneal articular facets of the talus in Korean. Korean journal of physical anthropology. 25(4), 185-192.
• Lee, U. Y., Han, S. H., Park, D. K., Kim, Y. S., Kim, D. I., Chung, I. H., et al. (2012a). Sex determination from the talus of Koreans by discriminant function analysis. Forensic Science International, 57, 166–171. https://doi.org/10.1111/j. 1556-4029.2011.01914.x PMID: 21981282.
• Lee, U. Y., Kim, I. B., Kwak, D. S. (2015). Sex determination using discriminant analysis of upper and lower extremity bones: New approach using the volume and surface area of digital model. Forensic Science International, 253-135. e1–135.e4. https://doi.org/10.1016/j.forsciint. 05.017 PMID: 26117502.
• Motagi, M. V., Kottapurath, S. R., Dharwadkar, K. (2015). Morphometric analyses of human dry tali of South Indian origin. International Journal of Medical Science and Public Health, 4(2), 237-240. doi: 10.5455/ijmsph.2015.3009201443.
• Naderi, S., Rezaei, H. R., Pompanon, F., Blum, M. G. B., Negrini, R., Naghash, H. R., Balkiz, O., Mashkour, M., Gaggiotti, O. E., Ajmone-Marsan, P., Kence, A., Vigne, J. D. Taberlet, P. (2008). The goat domestication process inferred from large-scale mitochondrial DNA analysis of wild and domestic individuals. Proceedings of the National Academy of Sciences, USA 105, 17659–17664. doi: 10.1073/pnas.0804782105.
• Nathena, D., Michopoulou, E., Kranioti, E. F. (2017). Sexual dimorphism of the calcaneus in contemporary Cretans. Forensic Science International, 277, 260. e1–260.e8. https://doi.org/10.1016/j. forsciint. 04.005 PMID: 28625510.
• Özkan, E. (2022). Kedilerde Pelvis ve Femur Radyolojik Görüntülerinin Osteometrik ve Geometrik Morfometrik Yöntemle Değerlendirilmesi. Doktora Tezi. İstanbul Üniversitesi-Cerrahpaşa Lisansüstü Eğitim Enstitüsü, Veterinerlik Anatomisi Anabilim Dalı Anatomi. İstanbul.
• Parés-Casanova, P. M. (2015). Geometric morphometrics to the study of skull sexual dimorphism in a local domestic goat breed. Journal of Fisheries & Livestock Production, 3(3), 141. doi:10.4172/2332-2608.1000141.
• Parés-Casanova, P. M., and Domènech-Domènech, X. (2021). A comparative analysis of sphenoid bone between domestic sheep (ovis aries) and goat (capra hircus) using geometric morphometrics. Anatomia, Histologia, Embryologia, 50, 556–561. doi: 10.1111/ahe.12661.
• Payne, W. J. A. and Wilson, R. T. (1999). An introduction to animal husbandry in the tropics (5th ed.). Blackwell Science, s: 824. Oxford.
• Rohlf, F. J. (2018) TpsDig Version 2.31, Ecology & Evolution. SUNY at Stone Brook, USA.
• Rohlf, F. J. (2019). TpsUtil program Version 1.79, Ecology & Evolution. SUNY at Stone Brook. USA.
• Slice, D. E. (2007). Geometric morphometrics, Annual Review Anthropol, 36(1), 261-281. DOI: 10.1146/annurev.anthro.34.081804.120613.
• Sorrentino, R., Belcastro, M. G., Figus, C., Stephens, N. B., Turley, K. (2020). Exploring sexual dimorphism of the modern human talus through geometric morphometric methods. PLoS ONE, 15(2), e0229255. https://doi. org/10.1371/journal.pone.0229255.
• Vermeulen, V. Kozma, E. Delsupehe, A. Pieter, C. Emmelie, S. Alexander, V. T. Lieven, D. W. Vereecke, E. (2022). Scapular morphology of great apes and humans: A three-dimensional computed tomography-based comparative study. Jornal of Anatomy. 242(2), 164-173. DOI: 10.1111/joa.13784.
• Yaprak, A., Demiraslan, Y., Özcan, Ö. (2022). Investigation of the skull basally in honamli, Hair, Kilis and Saanen Goats using geometric morphometric methods. Harran Üniversitesi Veteriner Fakültesi Dergisi, 11(2), 179-184. DOI:10.31196/huvfd.1161196.
• Zeder, M. A. and Hesse, B. (2000). The initial domestication of goats (Capra hircus) in the Zagros mountains 10, 000 years ago. Science, 287(5461), 2254-2257. doi: 10.1126/science.287.5461.2254.