Parasetamolün Sıçanlarda In Vitro Uterus Spontan Kasılmaları Üzerindeki Gevşetici Etkileri

Öz

Amaç: Parasetamol akut ve kronik ağrıda yaygın olarak kullanılan bir ilaçtır. Ağrı kesici ve ateş düşürücü bir ilaç olan parasetamolün gebelikte kullanımının güvenli olduğu bilinmektedir. Bu çalışmanın amacı, diöstrustaki dişi sıçanlarda parasetamolün uterus düz kas kasılma gevşeme mekanizması üzerindeki etkilerini araştırmaktır.
Materyal ve Metot: Çalışmada Wistar-albino intak dişi sıçanlar kullanıldı. Longitudinal miyometriyum şeritleri diöstrustaki hayvanlardan elde edildi. Şeritler, 1 g pasif gerilim altında krebs solüsyonu içeren izole organ banyosuna asıldı. Regülasyon periyodundan sonra, parasetamol 1000μM ve 2000μM konsantrasyonlarda kümülatif olmayan şekilde eklendi. Uygulama öncesi ve sonrası eğri altında kalan alan (AUC) ve zirveden zirveye (p-p) değerleri % değişim olarak normalize edilmiştir.
Bulgular: Parasetamol spontan uterus kontraksiyonlarının p-p ve eğri altında kalan alan parametrelerinde 1000 ve 2000 μM dozlarda istatistiksel olarak anlamlı azalmaya neden oldu (p <0.001).
Sonuçlar: Parasetamol uterus kasılmasını inhibe ederek uterus gevşemesine neden olur. Klinik kullanımında bu etki göz önünde bulundurulmalıdır.

Anahtar Kelimeler

• Parasetamol
• uterus
• izometrik kontraksiyon
• sıçan

Relaxing Effects of Paracetamol on Uterine Spontaneous Contraction in Rats in Vitro

Öz

Aim: Paracetamol is a commonly used drug in acute and chronic pain. It is known that paracetamol, which is a pain reliever and antipyretic drug, is safe to use during pregnancy. The aim of this study was to investigate the effects of paracetamol on the uterine smooth muscle contraction- relaxation mechanism in female rats in diestrus.
Material and Methods: Wistar-albino intact female rats were used in the study. Longitudinal strips of myometrium obtained from animals at the diestroeus stage. Stripes were suspended in an isolated organ bath containing crebs solution under 1 g passive tension. After the regulation period, paracetamol were added non-cumulatively at 1000μM and 2000μM concentrations. Before and after the application, the area under the curve (AUC) and peak to peak (p-p) values were normalized as % change.
Results: Paracetamol caused a statistically significant decrease in p-p and area under the curve parameters of spontaneous uterine contractions at 1000 and 2000 μM doses (p <0.001).
Conclusion: Paracetamol causes uterine relaxation by inhibiting uterine contraction. This effect should be taken into account in clinical use.

Anahtar Kelimeler

• Paracetamol
• Uterus
• Isometric Contraction
• Rat

Kaynakça

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