Biochemical and Histopathological Investigation of the Protective Effect of Lutein in Rat Kidney Exposed to Cisplatin

Abstract

Aim: The aim of this study was to determine the protection of lutein (L) (100 mg/kg, orally) against kidney damage caused by cisplatin (Cis) (10mg/kg, intraperitoneal). Materials and Methods: Twenty-eight rats aged 8 weeks were used and divided into four equal groups (n=7): Control, L, Cis, and Cis + L. Results: As a result of biochemical findings, serum Cr and BUN levels of the Cis group were significantly increased compared to the control group (p<0.05). Serum Cr and BUN levels of the Cis + L group were found to be significantly reduced compared to the Cis group (p<0.05). In addition, a decrease in MDA level and an increase in GSH, SOD and CAT levels were detected in the Cis + L group compared to the Cis group (p<0.05). In the histopathological examinations, in the Cis group kidney tissues; pathological changes such as interstitial mononuclear leukocyte infiltration, tubular degeneration, and tubular necrosis were observed. When the Cis + L group is compared with the Cis group, it can be said that there is a decrease in oxidative stress, an increase in antioxidant activity and a decrease in histopathological changes with the effect of L. Conclusion: These results show that L is effective in preventing Cis-induced kidney injury. It is also emerging that L is a pharmacological agent with the potential to be used in this damage.

Keywords

• Antioxidant
• kidney
• lutein
• cisplatin
• rat

Sisplatin’e Maruz Bırakılan Sıçan Böbreğinde Lutein’in Koruyucu Etkisinin Biyokimyasal ve Histopatolojik Olarak İncelenmesi

Öz

Amaç: Çalışmamızda, sisplatin (Cis) (10mg/kg, intraperitoneal) kaynaklı böbrek hasarına karşı lutein’in (L) (100mg/kg, oral) koruyucu etkisi incelendi. Materyal ve Metot: Bu çalışma, 8 haftalık 28 adet sıçanla gerçekleştirildi. Oluşturulan 4 grubun her birinde 7 tane sıçan yer aldı: Kontrol, L, Cis ve Cis + L. Bulgular: Biyokimyasal bulgulara göre, Cis grubunun kontrol grubu ile kıyaslanmasında serum Cr ve BUN seviyelerinin anlamlı düzeyde arttığı görüldü (p<0.05). Cis + L grubunda serum Cr ve BUN düzeylerinin Cis grubuna kıyasla anlamlı düzeyde azaldığı tespit edildi (p<0.05). Ayrıca Cis + L grubunun Cis grubu ile kıyaslanmasında MDA seviyesinde düşüş, GSH, SOD ve CAT seviyelerinde ise artış kaydedildi (p<0.05). Yapılan histopatolojik incelemelerde Cis grubu sıçanların böbrek dokularında interstisyel mononükleer lökosit infiltrasyonu, tubuler dejenerasyon ve tubuler nekroz gibi patolojik değişiklikler gözlendi. Cis + L grubu Cis grubu ile kıyaslandığında L’nin etkisi ile oksidatif streste bir azalma, antioksidan aktivitede bir artma ve histopatolojik değişikliklerde bir azalma olduğu söylenebilir. Sonuç: Bu sonuçlar L’nin Cis kaynaklı böbrek hasarının önlenmesinde etkili olduğunu göstermektedir. Ayrıca L’nin bu hasarda kullanılma potansiyeli olan bir farmakolojik ajan olduğuda ortaya çıkmaktadır.

Anahtar Kelimeler

• Antioksidan
• böbrek
• lutein
• sisplatin
• sıçan

Kaynakça

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