High Salt-Induced Hyperosmolality Reduces in Vitro Survival and Proliferation of Pre-B Cells

Yıl 2023, , 150 - 4, 19.10.2023

Mehmet Yabaş

https://doi.org/10.37990/medr.1352143

Öz

Aim: B cells of the adaptive immunity are critical for protection against the vast majority of pathogens through the production of specific antibodies. A number of signaling pathways and transcription factors control B cell development. Environmental factors, including diet, are also important in determining how B cell develop and function. Here, the effects of hyperosmolality induced by elevated salt on the survival, IL-7-induced proliferation and differentiation of pre-B cells were tested in vitro.
Material and Methods: The wk3 pre-B cell line generated from SLP65–/– mice was used. Hyperosmolality in the cell culture medium was created by increasing the salt concentration with the addition of 40 mM NaCl. Wk3 pre-B cells were cultured in standard (normal NaCl) and high salt (+40 mM NaCl) medium, followed by flow cytometric analysis.
Results: It was found that hyperosmolality caused by high salt reduced survival and induced apoptosis in wk3 pre-B cells. In addition, hyperosmolality inhibited IL-7-induced proliferation of pre-B cells. Conversely, pre-B cells treated with high salt were able to differentiate normally into IgM+ immature B cells when IL-7 was removed.
Conclusion: These findings suggest that the hyperosmolar microenvironment induced by high salt may play a key role in B cell development in the bone marrow.

Anahtar Kelimeler

Hyperosmolality, pre-B cells, salt, B cells

Etik Beyan

Not applicable

Teşekkür

The author thanks Dr. Sebastian Herzog, the Medical University of Innsbruck, Austria for providing wk3 cells, the Department of Genetics and Bioengineering at Trakya University, Turkey for the opportunity to conduct the experiments in their research facilities, and the Technology Research Development Application and Research Center of Trakya University, Turkey for the use of the flow cytometry.

Kaynakça

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