Can Mecsina Hemostopper, which has a Cytotoxic Effect on Mcf-7 Cells, be Considered an Anticarcinogenic Agent due to its Immunological Properties?

Abstract

Aim: Mecsina is used as a hemostatic agent such as Ankaferd and Tranexamic acid. To struggle with breast cancer, which is a serious public health issue, new, effective, and less toxic therapeutic agents are needed. Hence, it is aimed to compare and evaluate the effects of Mecsina and Ankaferd, both of which contain natural biomolecules in their structure, and synthetic Tranexamic acid on MCF-7 cells. Material and Method: For this study, MCF-7 immortalized cell lines were commercially purchased. The cells, 5000 cells per flask for each different dose group, were distributed to the 9 groups (mecsina 1:1, 1:2, 1:10, 1:50, 1:100, 1:200, 1:500, distilled water administered negative and control without any administration). Cytotoxicity, ELISA cytokine levels were evaluated, and flow cytometric analyzes were performed for each group using the XTT analysis method, after 24 hours of incubation. Results: A significant difference was observed between different doses of drug administration groups of Mecsina Hemostopper hemostatic agent in MCF-7 cells (p<0.001). Besides, cytokine levels were found to be significantly higher than those of other possible therapeutic agents. Conclusion: Mecsina Hemostopper has been found to have anti-tumoral activity in MCF-7 cancer cell lines by producing a hemostatic effect.

Keywords

• MCF-7
• cytotoxicity
• flow cytometry
• CD4+
• CD8+
• mecsina

Kaynakça

1. Donepudi MS, Kondapalli K, Amos SJ, Venkanteshan P. Breast cancer statistics and markers. J Cancer Res Ther. 2014;10:506-11.
2. Ferrini K, Ghelfi F, Mannucci R, Titta L. Lifestyle, nutrition and breast cancer: facts an presumptions for consideration. Ecancermedicalscience. 2015;23:557.
3. Lee SJ, Umano K, Shibamoto T, et al. Identification of volatile components in basil (Ocimum basilicum L.) and thyme leaves (Thymus vulgaris L.) and their antioxidant properties. Food Chem. 2007;91:131-7.
4. Goker H, Haznedaroglu IC, Ercetin S, et al. Haemostatic actions of the folkloric medicinal plant extract Ankaferd Blood Stopper. J Int Med Res. 2008;36:163-70.
5. Emes Y, Aybar B, Vural P, et al. Effects of hemostatic agents on fibroblast cells. Implant Dent. 2014;23:641-7.
6. Zhao Y, Xi C, Xu W, Yan J. Role of tranexamic acid in blood loss control and blood transfusion management of patients undergoing multilevel spine surgery: A meta-analysis. Medicine (Baltimore). 2021;100:e24678.
7. Çiçek M, Tumer MK. Investigation of the effect of a new hemostatic agent field messina hemostopper® on proliferation of calvarial osteoblasts and evaluation of cytotoxicity by different tests. Dicle Med J. 2018;45:291-6.
8. Bray F, Ferlay J, Soerjomataram I, et al. Global cancer statistics GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. Ca Cancer J Clin. 2018;68:394-424.

9. Liao S, Hu X, Liu Z, et al. Synergistic action of microwaveinduced mild hyperthermia and paclitaxel in inducing apoptosis in the human breast cancer cell line MCF-7. Oncol Lett. 2019;17:603-15.
10. Shi Y, Zhang L, Liu X, et al. Icotinib versus gefitinib in previously treated advanced non-small-cell lung cancer (ICOGEN): a randomised, double-blind phase 3 non-inferiority trial. Lancet Oncol. 2013;14:953-61.
11. Taylor WF, Jabbarzadeh E. The use of natural products to target cancer stem cells. Am J Cancer Res. 2017;7:1588-605.
12. Naeem A, Hu P, Yang M, et al. Natural products as anticancer agents: current status and future perspectives. Molecules. 2022;27:8367.
13. Safarzadeh E, Sandoghchian S, Baradaran B. Herbal. medicine as inducers of apoptosis in cancer treatment. APB. 2014;4:421-7.
14. AlZamil AF, AlQutub MN. The effect of different cigarette smoking levels on gingival crevicular fluid volume and periodontal clinical parameters in Saudi Arabia. Saudi Dent J. 2023;35:525-33.
15. Bhatia M. Apoptosis versus necrosis in acute pancreatitis. Am J Physiol Gastrointest Liver Physiol. 2004;286:189-96.
16. Franco BJ, Valdivia SJE, Zamudio MH, et al. Actin cytoskeleton participation in the onset of IL-1beta induction of an invasive mesenchymallike phenotype in epithelial MCF-7 cells. Arch Med Res. 2010;41:170-81.
17. Erdemir EO, Duran İ, Haliloglu S. Effects of smoking on clinical parameters and the gingival crevicular fluid levels of IL-6 and TNF-α in patients with chronic periodontitis. J Clin Periodontol. 2004;31:99-104.
18. Krelin Y, Voronov E, Dotan S, et al. Interleukin-1beta-driven inflammation promotes the development and invasivenes of chemical carcinogen-induced tumors. Cancer Res. 2007;67:1062-71.
19. Liu A, Li Y, Lu S, et al. Stanniocalcin 1 promotes lung metastasis of breast cancer by enhancing EGFR–ERK–S100A4 signaling. Cell Death Dis. 2023;14:395.
20. Xiao F, Li C, Lin Y, et al. Increased risk of periodontitis occurrence in patients with rheumatoid arthritis and its association with the levels of IL-1β and TNF-α in gingival crevicular fluid. Ann Palliat Med. 2021;10:9078-87.
21. Işler SC, Demircan S, Cakarer S, et al. Effects of folk medicinal plant extract Ankaferd Blood Stopper on early bone healing. J Appl Oral Sci. 2010;18:409-14.
22. Turtle CJ, Riddell SR, Maloney DG, et al. CD19 CAR – T cells of defined CD4 + : CD8 + composition in adult B cell ALL patients find the latest version : CD19 CAR – T cells of defined CD4 + : CD8 + composition in adult B cell ALL patients. J Clin Invest. 2016;126:2123-38.
23. Datta J, Berk E, Xu S, et al. Anti-HER2 CD4(+) T-helper type 1 response is a novel immune correlate to pathologic response following neoadjuvant therapy in HER2-positive breast cancer. Breast Cancer Res. 2015;17:71.
24. Sommermeyer D, Hudecek M, Kosasih PL, et al. Chimeric antigen receptor-modified T cells derived from defined CD8+ and CD4+ subsets confer superior antitumor reactivity in vivo. Leukemia. 2016;30:492-500.
25. Kowalczewska M, Piotrowski J, Jędrzejewski T, Kozak W. Polysaccharide peptides from Coriolus versicolor exert differential immunomodulatory effects on blood lymphocytes and breast cancer cell line MCF-7 in vitro. Immunol Lett. 2016;174:37-44.