Can Mecsina Hemostopper, which has a Cytotoxic Effect on Mcf-7 Cells, be Considered an Anticarcinogenic Agent due to its Immunological Properties?

Yıl 2024, Cilt: 6 Sayı: 1, 20 - 26, 31.01.2024

Mustafa Çiçek , Mehmet Kemal Tümer , Alpgiray Turgut

https://doi.org/10.37990/medr.1394291

Öz

Aim: Mecsina is used as a hemostatic agent such as Ankaferd and Tranexamic acid. To struggle with breast cancer, which is a serious public health issue, new, effective, and less toxic therapeutic agents are needed. Hence, it is aimed to compare and evaluate the effects of Mecsina and Ankaferd, both of which contain natural biomolecules in their structure, and synthetic Tranexamic acid on MCF-7 cells.
Material and Method: For this study, MCF-7 immortalized cell lines were commercially purchased. The cells, 5000 cells per flask for each different dose group, were distributed to the 9 groups (mecsina 1:1, 1:2, 1:10, 1:50, 1:100, 1:200, 1:500, distilled water administered negative and control without any administration). Cytotoxicity, ELISA cytokine levels were evaluated, and flow cytometric analyzes were performed for each group using the XTT analysis method, after 24 hours of incubation.
Results: A significant difference was observed between different doses of drug administration groups of Mecsina Hemostopper hemostatic agent in MCF-7 cells (p<0.001). Besides, cytokine levels were found to be significantly higher than those of other possible therapeutic agents.
Conclusion: Mecsina Hemostopper has been found to have anti-tumoral activity in MCF-7 cancer cell lines by producing a hemostatic effect.

Anahtar Kelimeler

MCF-7 , cytotoxicity , flow cytometry , CD4+ , CD8+ , mecsina

Teşekkür

The authors would like to thank Kahramanmaraş Sütçü İmam University Biomedical Research Center for their laboratory support.

Kaynakça

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