Venöz Yetmezliği Olan Hastalarda Oksidatif Stres Düzeyleri ile Total Oksidan ve Antioksidan Durumunun Değerlendirilmesi

Öz

Amaç: Venöz yetmezlik için çeşitli etiyolojik faktörlerin kökenine, ülserin yerine, genişliğine, derinliğine ve enfekte alanına bağlı olarak değişken tedavi seçenekleri bulunmaktadır. Bu çalışmada oksidatif stres yükünün belirlenmesi ve tüm süreçte multidisipliner bir yaklaşımın gerekliliğine, erken tanı ve tedavi olanaklarına dikkat çekilmesi amaçlanmıştır.Gereç ve Yöntem: Bu karşılaştırmalı gözlemsel çalışmada venöz yetmezliği olan ve olmayan bireylerde oksidatif stres parametreleri araştırıldı. Oksidatif stresin etkileri ELISA prensibine dayalı kitler kullanılarak serum 8-OHdG, MDA , TAS ve TOS testleri ile araştırılmıştır. Sayısal değişkenlerin normalliği Shapiro-Wilk testi ile, grup varyanslarının homojenliği ise Levene testi ile değerlendirilmiştir. Tanımlayıcı istatistikler için, sayısal değişkenler için ortalama, standart sapma (SD), medyan ve minimum-maksimum değerler hesaplanırken, kategorik değişkenler için frekans (n) ve yüzde (%) değerleri kullanılmıştır. Tüm analizlerde 0.05 Tip I hata düzeyi (α) istatistiksel olarak anlamlı kabul edilmiştir. İstatistiksel analizler IBM SPSS Statistics for Windows, Sürüm 30.0 (IBM Corp., Armonk, NY, ABD) kullanılarak gerçekleştirilmiştir.Sonuçlar: Çalışmaya %45,6'sı kadın ve %54,4'ü erkek olmak üzere toplam 68 hasta dahil edildi. 8-OHdG, TOS (µmol/L), TAS (µmol Trolox Equiv. /L) ve OSI (sırasıyla p=0.417,p=0.742,p=0.210,p=0.416) açısından istatistiksel olarak anlamlı bir fark bulunmazken, hasta grubunun MDA düzeyi (Sıra Ortalaması: 25.70) kontrol grubundan (Sıra Ortalaması: 38.10) anlamlı derecede düşük bulunmuştur (p=0.007). Sonuç: Oksidatif stres belirteçleri düzeylerine ilişkin literatürde etki mekanizmaları ve sonuçlar açısından farklılıklar bulunmaktadır. Oksidan ve antioksidan mekanizmalarla ilgili literatürdeki farklı sonuçlar, bu konuda çok daha geniş örneklemli ve analizli çalışmaların yapılması gerektiğine işaret etmektedir.

Anahtar Kelimeler

• Antioxidant capacity
• family medicine
• oxidant capacity
• oxidative stress
• venous insufficiency.

Assessment of Oxidative Stress Levels and Total Oxidant and Antioxidant Status in Patients with Venous Insufficiency

Abstract

Aim: There are variable treatment options for venous insufficiency depending on the origin of the various etiologic factors, the location, width, depth and infected area of the ulcer.In this study, we aimed to determine the oxidative stress load and to draw attention to the need for a multidisciplinary approach in the whole process as well as early diagnosis and treatment opportunities. Material and Method: This comparative observational study to investigate oxidative stress parameters in individuals with and without venous insufficiency. Effects of oxidative stress were investigated by serum 8-OHdG, MDA , TAS, and TOS tests using kits based on the ELISA principle. The normality of numerical variables was assessed using the Shapiro–Wilk test, while the homogeneity of group variances was evaluated with Levene’s test. For descriptive statistics, mean, standard deviation(SD), median, and minimum–maximum values were calculated for numerical variables, whereas frequency(n) and percentage(%) values were used for categorical variables. A Type I error level(α) of 0.05 was considered statistically significant in all analyses. Statistical analyses were conducted using IBM SPSS Statistics for Windows, Version 30.0 (IBM Corp., Armonk, NY, USA). Results: A total of 68 patients, 45.6% female and 54.4% male, were included in the study.While there was no statistically significant difference between the groups in terms of 8-OHdG, TOS (µmol/L), TAS (µmol Trolox Equiv./L) and OSI (p=0.417,p=0.742,p=0.210,p=0.416, respectively), the MDA level of the patient group (Rank Mean: 25.70) was significantly lower than the control group (Rank Mean: 38.10) (p=0.007). Conclusion: There are variations in the literature on oxidative stress markers levels in terms of mechanisms of action and outcomes. The varied results in the literature on oxidant and antioxidant mechanisms indicate that studies with much larger samples and analyses should be conducted on this subject.

Keywords

• Antioxidant capacity
• family medicine
• oxidant capacity
• oxidative stress
• venous insufficiency.

Kaynakça

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