Cisplatin ile İndüklenen Testis Hasarında Timokinon’un Anti-Apoptotik Etkileri: Cleaved Caspase-3 Ekspresyonuna Odaklanma

Öz

Amaç: Etkili bir kemoterapötik ajan olan Cisplatin, spermatogenezi bozan ve infertiliteye neden olabilen testis hasarı da dahil olmak üzere birçok yan etkiye sahiptir. Biyolojik özellikleri nedeniyle giderek daha fazla araştırılan Timokinon’un testis dokusu üzerindeki Cisplatin'in olumsuz etkilerini ortadan kaldırmayı veya en aza indirmeyi amaçladık. Materyal ve Yöntem: Bu amaçla 29 Wistar albino rat rastgele Kontrol (n = 4), Sham (n = 4), Cisplatin (n = 7), Cisplatin + Timokinon (n = 7) ve Timokinon (n = 7) gruplarına ayrıldı. Sham grubuna 10 gün boyunca gavaj yoluyla mısır yağı verildi. Cisplatin ve Cisplatin + Timokinon gruplarına 3., 5. ve 7. günlerde Cisplatin (1,5 mg/kg/gün, intraperitoneal) verilirken, Cisplatin + Timokinon ve Timokinon gruplarına 10 gün boyunca günlük olarak Timokinon (50 mg/kg/gün, oral) uygulandı. 10. günde, testis dokuları anestezi altında çıkarıldı ve Cleaved Caspase-3 antikoru kullanılarak histolojik ve immünohistokimyasal analizler için işlendi. Sonuçlar: Cisplatin + Timokinon grubunda, Cisplatin grubuna kıyasla apoptozisi gösteren Cleaved Caspase-3 antikorunun ekspresyonunda önemli bir azalma gözlendi. Timokinon'un Cisplatin'in neden olduğu testis hasarını ve germ hücre apoptozisini azalttığı belirlendi. Sonuç: Bu sonuçlar Timokinon’un, Cisplatin'in testis dokusunda neden olduğu hasarı onarmada ve spermatid apoptozisini azaltmada etkili olduğunu göstermektedir, ancak kemoprotektan olarak kullanılabilmesi için yeni çalışmalarla desteklenmesi gerekmektedir.

Anahtar Kelimeler

• Antioksidan
• Cisplatin
• Cleaved Caspase-3
• Timokinon
• Apoptozis

Anti-Apoptotic Effects of Thymoquinone in Cisplatin-Induced Testicular Injury: A Focus on Cleaved Caspase-3 Expression

Öz

Aim: Cisplatin, an effective chemotherapeutic agent, has many side effects, including testicular damage that impairs spermatogenesis and may cause infertility. We aimed to eliminate or minimize the negative effects of Cisplatin on testicular tissue with Thymoquinone, which is increasingly investigated due to its biological properties. Material and Method: For this purpose, 29 Wistar albino rats were randomly divided into Control (n = 4), Sham (n = 4), Cisplatin (n = 7), Cisplatin + Thymoquinone (n = 7), and Thymoquinone (n = 7) groups. Corn oil was given to the Sham group via gavage for 10 days. The Cisplatin and Cisplatin + Thymoquinone groups received Cisplatin (1.5 mg/kg/day, intraperitoneally) on days 3, 5, and 7, while Thymoquinone (50 mg/kg/day, orally) was administered daily for 10 days to the Cisplatin + Thymoquinone and Thymoquinone groups. On day 10, testicular tissues were removed under anesthesia and processed for histological and immunohistochemical analyses using Cleaved Caspase-3 antibody. Results: A significant decrease was observed in the expression of the Cleaved Caspase-3 antibody, which indicates apoptosis, in the Cisplatin + Thymoquinone group compared to the Cisplatin group. It was determined that Thymoquinone reduced testicular damage and germ cell apoptosis caused by Cisplatin. Conclusion: These results show that Thymoquinone is effective in repairing the damage caused by Cisplatin in testicular tissue and reducing spermatid apoptosis, but it needs to be supported by new studies to be used as a chemoprotectant.

Anahtar Kelimeler

• Antioxidant
• Cisplatin
• Cleaved Caspase-3
• Thymoquinone
• Apoptosis

Kaynakça

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