Helichrysum arenarium (L.) Moench Ekstraktlarının Çözücü Bağlı Antioksidan Profilleri: İn Vitro Karşılaştırmalı Değerlendirmesi

Öz

Helichrysum arenarium (L.) Moench, Asteraceae familyasına ait çok yıllık otsu bir bitkidir ve Avrupa ve Asya'da yaygın olarak dağılmıştır. İçerdiği fitokimyasallar nedeniyle tıpta kullanılmaktadır. Bu çalışmada etanol, su ve kloroform çözücüleri kullanılarak ekstraktlar elde edilmiş ve elde edilen ekstraktların antioksidan potansiyelleri çeşitli in vitro testlerle belirlenmiştir. DPPH radikal süpürme aktivitesi testinde etanol ekstresi diğer ekstrakt örneklerine göre daha yüksek inhibisyon göstermiştir. Etanol, kloroform ve su için Ec50 değerleri sırasıyla 16.60 µg/mL, 85.67 µg/mL ve 175.86 µg/mL olarak belirlenmiştir. Bu durumun ekstraksiyon sırasında açığa çıkan fenolik bileşiklerin oranı ile ilgili olduğu düşünülmektedir. Ayrıca elde edilen ekstrakt örnekleri orta düzeyde Nitrik oksit, süperoksit ve hidrojen peroksit temizleme aktivitesine sahiptir. Bu bulgular, H. arenarium'un potansiyel bir doğal antioksidan kaynağı olarak hizmet edebileceğini göstermektedir.

Anahtar Kelimeler

• Helichrysum arenarium
• antioksidan
• özüt
• tıp

Solvent-Dependent Antioxidant Profiles of Helichrysum arenarium (L.) Moench Extracts: A Comparative In Vitro Assessment

Öz

Aim: Helichrysum arenarium (L.) Moench is perennial herbaceous plant belongs to the Asteraceae family and is widely distributed in Europe and Asia. It is used in medicine due to its phytochemicals. Material and Metod: In the current study, extracts were obtained using ethanol, water and chloroform solvents, and the antioxidant potentials of the obtained extracts were determined by various in vitro tests. Results: In the DPPH radical scavenging activity test the ethanol extract showed higher inhibition than the other extract samples. The Ec50 values for ethanol, chloroform, and water was determined as 16.60 µg/mL, 85.67 µg/mL and 175.86 µg/mL respectively. This is thought to be related to the ratio of phenolic compounds released during extraction. Also the obtained extract samples has moderate Nitric oxide, superoxide and hydrogen peroxide scavenging activity. Conclusion: These findings suggest that H. arenarium may serve as a potential natural source of antioxidants.

Anahtar Kelimeler

• Helichrysum arenarium
• antioxidant
• extract
• medicine

Kaynakça

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